A Steady State of CD4+ T Cell Memory Maturation and Activation Is Established during Primary Subtype C HIV-1 Infection

Maenetje, Pholo; Riou, Catherine; Casazza, Joseph P.; Ambrozak, David R.; Hill, Brenna J.; Gray, Glenda; Koup, Richard A.; Bruyn, Guy de; Gray, Clive M.

doi:10.4049/jimmunol.0903771

Cited by 23 publications

(25 citation statements)

References 52 publications

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“…The copy numbers of the synthetic HIV-1 standard were calculated from its quantity and molecular weight. Triplicate reactions were run, and the number of template copies was calculated based on the standard curve (20).…”

Section: Cd11bmentioning

confidence: 99%

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

Qin

Cai

Pan

et al. 2013

J Virol

144

205

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Section: Cd11bmentioning

confidence: 99%

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

Qin

Cai

Pan

et al. 2013

J Virol

144

205

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“…51,52,58 In those latter studies, activated CD4 + T cells were compared to nonactivated CD4 + T cells, which presumably contained substantial proportions of naive CD4 + T cells, which contain approximately 10-fold less HIV-1 DNA than memory CD4 + T cells. 45,59 One study defined HLA-DR -CD25 -CD69 -as resting cells, 51 but we have found that this subset contains CD38…”

Section: Hiv-1 Dna In Non-gut-homing Cd4mentioning

confidence: 99%

The Majority of HIV Type 1 DNA in Circulating CD4⁺T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4⁺T Cells

McBride

Xu²,

Bailey³

et al. 2013

AIDS Research and Human Retroviruses

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Memory CD4+ T lymphocytes in peripheral blood that express integrins a4ß7 preferentially recirculate through gut-associated lymphoid tissue (GALT), a proposed site of significant HIV-1 replication. Tregs and activated CD4 + T cells in GALT could also be particularly susceptible to infection. We therefore hypothesized that infection of these subsets of memory CD4+ T cells may contribute disproportionately to the HIV-1 reservoir. A cross-sectional study of CD4 + T cell subsets of memory CD45RO + cells in peripheral blood mononuclear cells (PBMCs) was conducted using leukapheresis from eight subjects with untreated chronic HIV-1 infection. Realtime polymerase chain reaction (PCR) was used to quantify total and integrated HIV-1 DNA levels from memory CD4 + T cells sorted into integrin b7 + vs. b7 -, CD25 + CD127 low Treg vs. CD127 high , and activated CD38 + vs. CD38 -. More than 80% of total HIV-1 DNA was found to reside in the integrin b7-negative nongut-homing subset of CD45RO + memory CD4 + T cells. Less than 10% was found in highly purified Tregs or CD38+ activated memory cells. Similarly, integrated HIV-1 DNA copies were found to be more abundant in resting non-gut-homing memory CD4 + T cells (76%) than in their activated counterparts (23%). Our investigations showed that the majority of both total and integrated HIV-1 DNA was found within non-gut-homing resting CD4 + T cells.

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“…One study reported that HIV-1 gag DNA is elevated in activated peripheral blood memory CD4 ϩ T cells (defined as 38 ϩ , DR ϩ , or Ki67 ϩ cells that also expressed CD45RO) than in other memory CD4 ϩ T cells (31), suggesting that activated cells may be preferentially infected in vivo. Nevertheless, neither the proportion of gag DNA nor the amount of HIV-1 RNA produced by activated memory CD4 ϩ T cells was determined in this study.…”

mentioning

confidence: 99%

HLA-DR⁺CD38⁺CD4⁺T Lymphocytes Have Elevated CCR5 Expression and Produce the Majority of R5-Tropic HIV-1 RNAInVivo

Meditz

Haas

Folkvord

et al. 2011

J Virol

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A Steady State of CD4+ T Cell Memory Maturation and Activation Is Established during Primary Subtype C HIV-1 Infection

Abstract: Material

Cited by 23 publications

References 52 publications

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

The Majority of HIV Type 1 DNA in Circulating CD4⁺T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4⁺T Cells

HLA-DR⁺CD38⁺CD4⁺T Lymphocytes Have Elevated CCR5 Expression and Produce the Majority of R5-Tropic HIV-1 RNAInVivo

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A Steady State of CD4+ T Cell Memory Maturation and Activation Is Established during Primary Subtype C HIV-1 Infection

Abstract: Material

Cited by 23 publications

References 52 publications

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

Expansion of Monocytic Myeloid-Derived Suppressor Cells Dampens T Cell Function in HIV-1-Seropositive Individuals

The Majority of HIV Type 1 DNA in Circulating CD4+T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4+T Cells

HLA-DR+CD38+CD4+T Lymphocytes Have Elevated CCR5 Expression and Produce the Majority of R5-Tropic HIV-1 RNAInVivo

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Product

Resources

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The Majority of HIV Type 1 DNA in Circulating CD4⁺T Lymphocytes Is Present in Non-Gut-Homing Resting Memory CD4⁺T Cells

HLA-DR⁺CD38⁺CD4⁺T Lymphocytes Have Elevated CCR5 Expression and Produce the Majority of R5-Tropic HIV-1 RNAInVivo