A sterol biosynthetic geneAtCYP51A2 promoter for constitutive and ectopic expression of a transgene in plants

Lee, Hyoungseok; Oh, Hyun Joo; Ahn, Hyo Min; Oh, Chang Jae; Jeong, Jin Ho; Jeon, Gyeong Lyong; An, Chung Sun; Choi, Sang Bong; Kim, Ho Bang

doi:10.1007/bf03036139

Cited by 2 publications

(2 citation statements)

References 36 publications

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“…Because lanosterol 14α-demethylase (CYP51) is a prime target for the development of antifungal drugs , the study of CYP51 is becoming a research hotspot in agriculture , and the expression of CYP51 genes has been extensively investigated in Magnaporthe grisea, Penicillium digitatum, Antrodia cinnamomea, and Penicillium italicum − . Until now, a few expression systems, for example, Escherichia coli, Saccharomyces cerevisiae, Schizoasccharomyces pombe, and Pichia pastoris, and insect cell and mammalian cell systems were used for expressing CYPs.…”

Section: Introductionmentioning

confidence: 99%

“…There are only a few reports on the crystal structure of P450-14DM (CYP51), including the crystal structure of Mycobacterium tuberculosis CYP51 and the human and Trypanosoma brucei CYP51 − . Although some homology models such as CYP51 from M. grisea and P. digitatum were attained on the basis of the crystal structure of M. tuberculosis CYP51 , , this may not be a suitable template for the analysis of eukaryotic orthologues. Compared with the other two, the human CYP51 is the second structure of a eukaryotic microsomal P450-14DM that acts on sterol biosynthesis, and it differs profoundly from that of the water-soluble CYP51 family member from M. tuberculosis , both in organization of the active site cavity and in the location of substrate access channel.…”

Section: Introductionmentioning

confidence: 99%

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Homology Modeling and Screening of New 14α-Demethylase Inhibitor (DMI) Fungicides Based on Optimized Expression of CYP51 from Ustilago maydis in Escherichia coli

Han

Zhang

et al. 2010

J. Agric. Food Chem.

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Ustilago maydis infection is a serious disease affecting corn crops worldwide. Sterol 14α-demethylase (CYP51) is one of the key enzymes of sterol biosynthesis and an effective target of antifungal drugs. To further study the interaction between CYP51 and drugs and exploit more specific 14α-demethylase inhibitor (DMI) fungicides for U. maydis, in this study homology modeling of CYP51 from U. maydis (UmCYP51) templated as the eukaryotic orthologues (the human CYP51) and screening of new DMI fungicides based on optimized expression were carried out for the first time. In addition, XF-113 and ZST-4 were screened by analyzing the spectral characteristics between the purified UmCYP51-35 and fungicides. These results provide a theoretical basis and new ideas for efficient design and development of new antifungal drugs.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Homology Modeling and Screening of New 14α-Demethylase Inhibitor (DMI) Fungicides Based on Optimized Expression of CYP51 from Ustilago maydis in Escherichia coli

Han

Zhang

et al. 2010

J. Agric. Food Chem.

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Structure and Expression of OsUBP6, an Ubiquitin-Specific Protease 6 Homolog in Rice (Oryza sativa L.)

Moon

Hong

Cho

et al. 2009

Molecules and Cells

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Although the possible cellular roles of several ubiquitin-specific proteases (UBPs) were identified in Arabidopsis, almost nothing is known about UBP homologs in rice, a monocot model plant. In this report, we searched the rice genome database ( http://signal.salk.edu/cgi-bin/RiceGE ) and identified 21 putative UBP family members (OsUBPs) in the rice genome. These OsUBP genes each contain a ubiquitin carboxyl-terminal hydrolase (UCH) domain with highly conserved Cys and His boxes and were subdivided into 9 groups based on their sequence identities and domain structures. RT-PCR analysis indicated that rice OsUBP genes are expressed at varying degrees in different rice tissues. We isolated a full-length cDNA clone for OsUBP6, which possesses not only a UCH domain, but also an N-terminal ubiquitin motif. Bacterially expressed OsUBP6 was capable of dismantling K48-linked tetraubiquitin chains in vitro. Quantitative real-time RT-PCR indicated that OsUBP6 is constitutively expressed in different tissues of rice plants. An in vivo targeting experiment showed that OsUBP6 is predominantly localized to the nucleus in onion epidermal cells. We also examined how knock-out of OsUBP6 affects developmental growth of rice plants. Although homozygous T3 osubp6 T-DNA insertion mutant seedlings displayed slower growth relative to wild type seedlings, mature mutant plants appeared to be normal. These results raise the possibility that loss of OsUBP6 is functionally compensated for by an as-yet unknown OsUBP homolog during later stages of development in rice plants.

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A sterol biosynthetic geneAtCYP51A2 promoter for constitutive and ectopic expression of a transgene in plants

Cited by 2 publications

References 36 publications

Homology Modeling and Screening of New 14α-Demethylase Inhibitor (DMI) Fungicides Based on Optimized Expression of CYP51 from Ustilago maydis in Escherichia coli

Homology Modeling and Screening of New 14α-Demethylase Inhibitor (DMI) Fungicides Based on Optimized Expression of CYP51 from Ustilago maydis in Escherichia coli

Structure and Expression of OsUBP6, an Ubiquitin-Specific Protease 6 Homolog in Rice (Oryza sativa L.)

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