A Storage Technique for Cucurbit Powdery Mildew (Sphaerotheca Fuliginea).

Long‐term Preservation of Podosphaera fusca Using Silica Gel

“…In all cases, this maximum corresponded to desiccation periods of 6–12 h, which seemed to protect conidia from freezing damage to levels high enough to maintain viability of isolates of P. fusca at least for up to 1 year. This result improved twofold the viability previously reported for cryopreservation in liquid nitrogen of air‐dried conidia of P. fusca (O'Brien and Weinert, 1994). Hermansen (1972) described that conidia of B. graminis produced at high relative humidity lost their infectiveness after freezing, whereas conidia produced at low relative humidity retained their viability during storage at below‐freezing temperature.…”

“…Cryopreservation in liquid nitrogen has been considered the best and the most widely applicable preservation technique available for filamentous fungi (Smith, 1998), and it has been also reported for long‐term storage of P. fusca (O'Brien and Weinert, 1994; Bardin and Nicot, 1999). Briefly, conidia are dried in air or using a desiccating agent such as CaCl 2 , and stored at −196°C in liquid nitrogen.…”

The powdery mildew fungus Podosphaera fusca (synonym Podosphaera xanthii), a constant threat to cucurbits

“…As a conservation system, this method is not very practical for maintaining large numbers of isolates and does not prevent genetic or physiological changes during long-term and frequent subculturing (Nicot et al ., 2002). Cryopreservation in liquid nitrogen is considered to be the best and most widely applicable preservation technique available for filamentous fungi, and has also been reported for the long-term storage of P. fusca (Bardin et al ., 2007;O'Brien and Weinert, 1994). Briefly, conidia are dried in air or using a desiccating agent such as CaCl 2 , and stored at − 196 ° C in liquid nitrogen.…”

Labor-saving Preservation of Powdery Mildew of Strawberry by Sterilized Seedling Culture

“…The conidia transferred to WA from liquid nitrogen did not germinate. The result suggests that P. aphanis cannot be preserved in liquid nitrogen, although the fungus causing powdery mildew of cucurbits can be preserved in liquid nitrogen 10 . One of the most popular methods for the long-term preservation of the fungi causing powdery mildew is to use cleistothecia, which are small saclike bodies containing ascospores.…”