A strategy to engineer vascularized tissue constructs by optimizing and maintaining the geometry

Hsu, Yi-Jung; Wei, Shih-Yen; Lin, Teng-Yen; Fang, Ling; Hsieh, Yu-Chung; Chen, Ying-Chieh

doi:10.1016/j.actbio.2021.11.003

Cited by 8 publications

(2 citation statements)

References 73 publications

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“…HUVECs and MSCs commonly used in engineering three-dimensional (3D) vascularized constructs were used as model cells to investigate cellular responses inside collagen-based hydrogel scaffolds. Crosslinkers were always considered harmful to cells, so the conditions used for the photo-crosslinking process (0.125% (w/v) photoinitiator (PI) and 0–60 s of UV irradiation) were first confirmed nontoxic for both HUVECs and MSCs 29 (viability >90%; Figure S3(a)–(d) ). To evaluate the biocompatibility to of our collagen-based hydrogels to cells, NIH3T3, HUVECs and MSCs cells were seeded on the top of preformed Collagen and CMA-15 gels and cultured in a two-dimensional environment.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, MSCs and HUVECs were directly co-cultured at a 1:1 cell ratio in the cell density of 1 × 107 cells/mL, a suitable combination to generate vascular networks. 26 , 28 – 30 For the cell-laden Collagen group, the collagen prepolymer solutions (100 μL) were mixed with MSCs and HUVECs (1:1 ratio; 1 × 107 cells/mL) before gelation. The cell-laden CMA mixture (100 μL) was pipetted onto a polydimethylsiloxane-coated glass slide, with aliquots separated using a 1 mm spacer following 15 and 20 s of UV light exposure to form the cell-laden CMA-15 and CMA-20 groups, respectively.…”

Section: Methodsmentioning

confidence: 99%

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Reconstructing vascular networks promotes the repair of skeletal muscle following volumetric muscle loss by pre-vascularized tissue constructs

Chen,

Wei,

Chen

et al. 2023

J Tissue Eng

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Current treatment for complex and large-scale volumetric muscle loss (VML) injuries remains a limited success and have substantial disadvantages, due to the irreversible loss of muscle mass, slow muscle regeneration, and rapid formation of non-functional fibrosis scars. These VML injuries are accompanied by denervation and the destruction of native vasculature which increases difficulties in the functional restoration of muscle. Here, reconstruction of the vascular network at the injury site was offered as a possible solution for improving the repair of muscle defects through the timely supply of nutrients and oxygen to surrounding cells. A hydrogel-based tissue construct containing various densities of the vascular network was successfully created in the subcutaneous space of mice by manipulating hydrogel properties, and then implanted into the VML injury site. One month after implantation, the mouse treated with the highly vascularized tissue had extensive muscle repair at the injury site and only spent a shorter time completing the inclined plane tests. These findings suggest that the reconstruction of the functional vascular network at the VML injury site accelerated muscle fiber repair through a timely supply of sufficient blood and avoided invasion by host fibroblasts.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Reconstructing vascular networks promotes the repair of skeletal muscle following volumetric muscle loss by pre-vascularized tissue constructs

Chen,

Wei,

Chen

et al. 2023

J Tissue Eng

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Enhancing the Repair of Substantial Volumetric Muscle Loss by Creating Different Levels of Blood Vessel Networks Using Pre‐Vascularized Nerve Hydrogel Implants

Wei,

Chen,

Tsai

et al. 2024

Adv Healthcare Materials

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Volumetric muscle loss (VML) is a significant loss of muscle tissue due to trauma or surgery, resulting in scarring, minimal regeneration, and notable fibrosis. This leads to permanent declines in muscle mass and function. One potential approach to enhance recovery in substantial VML is to restore vascular and neural networks at the injury site. However, the processes of neovascularization and tissue integration at the muscle injury site have not been widely studied. Collagen hydrogels have been explored as scaffolds for cultivating cell‐mediated blood vessels due to their biocompatibility. However, effectively reconstructing blood vessels and guiding host neurons for innervation at the injury site remains challenging. In this study, collagen hydrogels with varying blood vessel‐forming cell densities were subcutaneously implanted in mice, creating pre‐vascularized hydrogels with a wide range of vessel densities (0∼145 numbers/mm2) within a week. Host motor neurons concurrently followed these pre‐formed vessels through coordinated interactions, forming pre‐vascularized nerve hydrogels. These hydrogels were then transplanted into muscle injury sites and evaluated for their muscle repair capabilities through histological and behavioral analyses. The results demonstrated that achieving sufficiently high microvessel densities within the hydrogel construct, which fully occupied the wound area, effectively reconnected with the host vasculature and interacted with neural networks to promote neovascularization, myogenesis, and integration processes, ultimately addressing approximately 63% of the volumetric muscle loss.This article is protected by copyright. All rights reserved

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Engineering large and geometrically controlled vascularized nerve tissue in collagen hydrogels to restore large-sized volumetric muscle loss

Wei,

Chen,

Hsieh

et al. 2023

Biomaterials

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A strategy to engineer vascularized tissue constructs by optimizing and maintaining the geometry

Cited by 8 publications

References 73 publications

Reconstructing vascular networks promotes the repair of skeletal muscle following volumetric muscle loss by pre-vascularized tissue constructs

Reconstructing vascular networks promotes the repair of skeletal muscle following volumetric muscle loss by pre-vascularized tissue constructs

Enhancing the Repair of Substantial Volumetric Muscle Loss by Creating Different Levels of Blood Vessel Networks Using Pre‐Vascularized Nerve Hydrogel Implants

Engineering large and geometrically controlled vascularized nerve tissue in collagen hydrogels to restore large-sized volumetric muscle loss

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