A streptomycin-resistant line of Nicotiana sylvestris unable to flower

Abstract

A streptomycin-resistant cell line, SR155, was selected in a callus culture initiated from diploid Nicotiana sylvestris. Regenerates of the SR155 line have a grossly altered morphology, an altered peroxidase isoenzyme pattern, and do not flower. The SR155 plants are diploid, but in the karyotype a chromosome translocation was detected.

Asulam Resistance in Embryoids of Celery Tissue Cultures

“…Since this was the case in both selected and unselected plants, all of which were dwarfed, it suggested that the tissue culture process was the cause of the non-viability of the seed rather than the Asulam selection. The poor reproductive capacity of tissue culture derived plants has been observed before (Aviv and Galun, 1977;Maliga et al, 1979) and the factors responsible require further investigation. Unfortunately, in this project lack of seed formation prevented any testing of the progeny for inheritance of Asulam resistance.…”