A Study in Vivo of the Effects of a Static Compressive Load on the Proximal Tibial Physis in Rabbits

Bries, Andrew D.; Weiner, Dennis S.; Jacquet, Robin; Adamczyk, Mark J.; Morscher, Melanie A.; Lowder, Elizabeth; Askew, Michael J.; Steiner, Richard; Horne, Walter I.; Landis, William J.

doi:10.2106/jbjs.k.00340

Cited by 19 publications

(22 citation statements)

References 35 publications

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“…The powder was transferred to a sterile tube containing 3 to 5 mL of TRI Reagent (Molecular Research Center, Cincinnati, Ohio), and total RNA was isolated according to the manufacturer's protocol. The total RNA was purified with a PerfectPure RNA Tissue Kit (5 PRIME, Gaithersburg, Maryland) 12 . Purified RNA was shipped on dry ice to the Biomedical Genomics…”

Section: Methodsmentioning

confidence: 99%

Gene Expression Differences Between Ruptured Anterior Cruciate Ligaments in Young Male and Female Subjects

Johnson

Morscher

Jones

et al. 2015

The Journal of Bone and Joint Surgery

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Section: Methodsmentioning

confidence: 99%

Gene Expression Differences Between Ruptured Anterior Cruciate Ligaments in Young Male and Female Subjects

Johnson

Morscher

Jones

et al. 2015

The Journal of Bone and Joint Surgery

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“…Individual harvested specimens were divided in half with one portion placed in 10% neutralbuffered formalin for histological analysis and the other in RNAlater for reverse transcription-quantitative PCR to determine human-and murine-specific gene expression related to sample remodeling. Specimens placed in RNAlater were ground to powders under liquid nitrogen and purified total RNA was obtained by previous methods (11). Total RNA was reverse-transcribed and qPCR was completed after 40 cycles of the standard protocol on an Applied Biosystems 7500 instrument (Life Technologies, Carlsbad, CA).…”

Section: Methodsmentioning

confidence: 99%

“…Sample quantities were normalized to ribosomal protein (P0) and plots of fold change compared 20-to 10-week gene expression data. Gene data were statistically analyzed by a student t-test with errors given as standard errors of mean values and p50.05 was considered significant (11).…”

Section: Methodsmentioning

confidence: 99%

The character of gene expression of human periosteum used to form new tissue in allograft bone

Kemppainen

Alexander³

et al. 2014

Connective Tissue Research

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Of more than 2 million segmental bone defects repaired annually with bone autografts and allografts, 15-40% fail. Improving healing rates may be approached with tissue engineering and use of periosteum overlying an allograft. The present study documents gene expression in human periosteum-allograft constructs compared to allografts alone. Strips of human cadaveric periosteum (26 years, f, distal femur) were sutured about sterilized human femoral cortical strut bone allograft (54 years, m) segments. After construct incubation (M199 supplemented medium) for 8 d, constructs and allografts alone were implanted in nude mice. At 10 and 20 weeks, constructs (N = 4, each group) and allografts (N = 2, each group) were retrieved and placed in RNAlater for quantitative PCR to determine expression of human- and murine-specific genes relevant to remodeling. Specimens were frozen-ground to powders and RNA was extracted, purified, reverse-transcribed, and amplified. Ribosomal protein (P0) was used to normalize sample quantities. Fold change plots were generated following statistical analyses comparing 20- to 10-week gene expression data. Allografts alone yielded no human-specific gene expression. Notable fold changes of human-specific alkaline phosphatase, bone sialoprotein, type I collagen, decorin, RANKL, RANK, cathepsin K, and osteocalcin in 20-week compared to 10-week specimens were found. Murine-specific expression of genes indicative of host mouse vascularization (RANK, type I collagen) was detected in both allograft alone and periosteum-allograft samples. Gene data confirm viable periosteum in constructs after 20 weeks. Relatively higher fold-change values of RANK, RANKL and cathepsin K indicate activities of osteoclast precursors, osteoclasts and osteoblasts involved in allograft remodeling during implantation. All additional genes of interest indicate osteoblast activity in new bone matrix formation. Gene data are directly correlated with previous and present histology work. The results of this study suggest that further investigations could help to establish whether autologous periosteum-allograft constructs could be used for the repair of bone defects.

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“…Our study found that as stimulation increased in compression days, chondrocytes tended to hypertrophy that showed gene expression of Col-10 and MMP-13 increased obviously, Col-2 was degradation and the structural integrity of chondrocytes was damaged. Although these four genes (Agg, Col-2, Col-10 and MMP-13) demonstrated changes critical for physeal structural integrity and function, it is likely that the broader effects of static compression on rabbit physes would have been forthcoming if additional genes and the counterpart secreted molecules had been assessed 15) . Studies of cell mechanics found that the mechanical properties of chondrocytes could significantly affect the physiological function of the articular cartilage.…”

Section: Differences In Viscoelastic Properties Of Chondrocytes On DImentioning

confidence: 99%

Investigation for Effects of Cyclical Dynamic Compression on Matrix Metabolite and Mechanical Properties of Chondrocytes Cultured in Alginate

Zhou

Liu

Yang

et al. 2016

J. Hard Tissue Biology.

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Articular cartilage is suffered from high mechanical loads under normal physiological conditions and articular chondrocytes regulate the composition of peri-cellular matrix (PCM), in response to mechanical signals. This study aims to investigate influence of cyclical dynamic compression on matrix metabolite and mechanical properties of chondrocytes cultured in alginate. Two months old New Zealand white rabbits were sacrificed by air embolism. Chondrocytes were taken from knee joints under aseptic conditions and cultured in alginate. The suspension was gelled in a shape of disc in CaCl 2 solution and processed for intermittent compression by using Flexcell-5000 compression system kept under an uncompressed condition as a control. The gene regulation of aggrecan (Agg), collagen (Col-2), matrix metallo-proteinases 13 (MMP-13) and collagen X (Col-10) were determined by reverse transcription polymerase chain reaction (RT-PCR) at 7th, 14th and 21st day post loading. The alteration in mechanical properties of chondrocytes induced by compression was evaluated using micropipette aspiration technique combined with a half-space model. A significant up-regulation was observed in gene expression of Agg and Col-2 on 7th day or MMP-13 and Col-10 on 14th day respectively after loading of compression. In response to compression, chondrocytes exhibited viscoelastic solid creep behavior. Young's modulus almost approximated to control group (0.65±0.42kPa). However, viscoelastic properties decreased gradually along with intermittent compression. Viscoelastic properties in experimental 21st day, including equilibrium modulus (E ), the instantaneous modulus (E 0 ) and apparent viscosity (μ) were significantly lower than experimental 7th day and 14th day (P<0.001), no significant difference was found between experimental 7th day and 14th day (P >0.05). In conclusion, chondrocytes cultured in alginate are stable of mechanical properties and suitable to construct seed cell of tissue engineering, and can keep phenotype and mechanical properties.

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A Study in Vivo of the Effects of a Static Compressive Load on the Proximal Tibial Physis in Rabbits

Cited by 19 publications

References 35 publications

Gene Expression Differences Between Ruptured Anterior Cruciate Ligaments in Young Male and Female Subjects

Gene Expression Differences Between Ruptured Anterior Cruciate Ligaments in Young Male and Female Subjects

The character of gene expression of human periosteum used to form new tissue in allograft bone

Investigation for Effects of Cyclical Dynamic Compression on Matrix Metabolite and Mechanical Properties of Chondrocytes Cultured in Alginate

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