A study of adenosine 3′:5′-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase

Griffin, Michael K.; Price, G.H.; Bazzell, Kirstan L.; Cox, Rody P.; Ghosh, Nimai K.

doi:10.1016/0003-9861(74)90073-3

Cited by 110 publications

(15 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Butyrate also inhibits growth and induces morphologic changes in certain mammalian epithelial cells. Further, HeLa cells respond to butyrate exposure by increases in membranebound alkaline phosphatase activity (5) and by production of human chorionic gonadotrophin (6). Although the mechanism by which butyrate inhibits cell growth and induces changes in specific gene expression remains unclear, the structural requirements for these effects appear to be specific with regard to the chain length of the fatty acid (1).…”

Section: Introductionmentioning

confidence: 95%

Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Abe

Küfe

1984

Breast Cancer Res Tr

View full text Add to dashboard Cite

Sodium butyrate is a potent inducer of leukemic cell differentiation. This agent also affects epithelial cell proliferation and gene expression. The present study demonstrates that sodium butyrate slows proliferation and induces morphologic changes in human MCF-7 breast carcinoma cells. The results also demonstrate that butyrate-treated MCF-7 cells have enhanced expression of carcinoembryonic antigen. These effects are related to butyrate concentration and duration of exposure. The present findings may provide a model for studying maturation of human breast cancer cells.

show abstract

Section: Introductionmentioning

confidence: 95%

Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Abe

Küfe

1984

Breast Cancer Res Tr

View full text Add to dashboard Cite

show abstract

“…In addition to its effects on erythroid induction in Friend cells [ 11 J , sodium butyrate also affects granulopoiesis of mastocytoma cells [33], and causes induction of alkaline phosphatase [34] , morphologic transformations, and altered ganglioside sialyltransferase activity [35] in HeLa cells. Sodium butyrate inhibited cytochalasin B-induced multinucleation in NRK (normal rat kidney) cells [36].…”

Section: Levermentioning

confidence: 99%

Regulation of dome formation in differentiated epithelial cell cultures

Lever

1979

J Supramol Struct

View full text Add to dashboard Cite

Rat mammary (Rama 25) and dog kidney (MDCK) epithelial cell cultures formed 'domes' of cells due to fluid accumulation in focal regions between the culture dish and the cell monolayer. Addition of ouabain caused collapse of domes, suggesting that transport functions were required for maintenance of domes. Dome formation in both epithelial cell lines was stimulated by a broad spectrum of known inducers of erythroid differentiation in Fried erythroleukemia cells. Among these inducers were: 1) polar solvents such as dimethyl-sulfoxide, dimethylformamide, and hexamethylene bisacetamide; 2) purines such as hypoxanthine, inosine, and adenosine; 3) low-molecular-weight fatty acids such as n-butyrate; and 4) conditions expected to elevate levels of cyclic AMP. In the latter group were activators of adenylate cyclase such as cholera toxin and prostaglandin E 1; cyclic AMP phosphodiesterase inhibitors such as theophylline and 1-methyl-3-isobutylxanthine; and analogs of cyclic AMP. Induction of domes occurred 15--30 h after addition of inducer to the culture medium. Induction by chemicals was serum-dependent and required protein synthesis but not DNA synthesis. Induced dome formation was reversible after removal of inducer, requiring the continuous presence of inducer. Reversal was also observed after either either removal of serum or addition of inhibitors of protein synthesis. These results suggest that hypothesis that domes arise in these epithelial cultures by a process that is similar to cell differentiation and is influenced by cyclic AMP.

show abstract

“…In recent years, sodium butyrate has been found to induce various changes in mammalian cells in culture (13)(14)(15)(16)(17)(18)(19)(20).…”

mentioning

confidence: 99%

Induction of functional beta-adrenergic receptors in HeLa cells.

Tallman¹,

Smith²,

Henneberry³

1977

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

HeLa cells contain fl-adrenergic receptors that are characterized by specific binding of 1{3H]dihydroalprenolol, increased 3':5'-cyclic AMP production in intact cells after incubation with 1-isoproterenol, and increased adenylate cyclase [ATP pyrophosphate-lyase (cyclizing) (6,7) and supersensitivity after denervation and other manipulations (8, 9) and the role of modulators in the production of intracellular "second messengers."Because of the widespread distribution of fl-adrenergic receptors, which are important in catecholamine-mediated physiological events, and the apparent tight coupling between these receptors and adenylate cyclase [ATP pyrophosphatelyase (cyclizing), EC 4.6.1.11, this system is a valuable model for the study of biochemical factors regulating receptors. Through the use of radioactive fl-adrenergic antagonists it has been possible to characterize unambiguously a binding site of the fl-adrenergic receptor in various cell types and to correlate its binding characteristics with studies of fl-receptor-dependent activation of adenylate cyclase (7,8,(10)(11)(12). Because it is possible to study the binding site of the fl-adrenergic receptor separately from the ability of fl-adrenergic agonists to activate adenylate cyclase, independent evaluation of the number of receptors and their ability to function can be made (12).To further elucidate the regulatory mechanisms controlling Abbreviation: cAMP, adenosine 3':5'-cyclic monophosphate.

show abstract

A study of adenosine 3′:5′-cyclic monophosphate, sodium butyrate and cortisol as inducers of HeLa alkaline phosphatase

Cited by 110 publications

References 15 publications

Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Effect of sodium butyrate on human breast carcinoma (MCF-7) cellular proliferation, morphology, and CEA production

Regulation of dome formation in differentiated epithelial cell cultures

Induction of functional beta-adrenergic receptors in HeLa cells.

Contact Info

Product

Resources

About