A Study of Artificial Thrombi Produced by a Modification of Chandler's Method

Massive Thrombosis Produced by Fatty Acid Infusion*

“…The plasma FFA concentration was 517 ± 218 uM before the infusions of long-chain, saturated fatty acids and 490 4 170 ,uM immediately afterward (p > 0.5). With (5,6). The toxic effect on the myocardium, likewise limited to the long-chain, saturated fatty acids, was distinct from the induced intravascular clotting.…”

“…1 The thrombi produced in this system form in a moving stream of blood and are similar in structure to thrombi occurring in human thromboembolic disease (5). ports has thrombosis been alluded to as a pathological consequence of fatty acid infusion, nor has any differentiation been made between effects from different fatty acids.…”

The Acceleration of Thrombus Formation by Certain Fatty Acids*

“…When this in vitro thrombus-producing system is suitably modified, the time required for formation of a thrombus is highly reproducible (12,13). The use of this system then provides another tool * Presented in part at the Annual Meeting of the Federation of American Societies for Experimental Biology, Atlantic City, N. J., April 12, 1961 (1).…”

An Artificial Circulation for the Study of Thrombolysis

“…The fre quently used Chandler's tube permits the preparation of an artificial thrombus with the histological characteristics of an in vi vo thrombus [2,9], and rotation may be continued to allow study of thrombolysis after addition of fibrinolytic agents. Experiments with a perfusion circuit to study streptokinase-induced thrombolysis have been described by Kakkar [4], The ar tificial circulation described here enables the effect of variations in flow rate and pressure to be studied, allows frequent sampling of the perfusion fluid, and per mits lysis of different types of fibrin de posit to be examined.…”