A study of branched‐chain amino acid aminotransferase and isolation of mutations affecting the catabolism of branched‐chain amino acids in <i>Saccharomyces cerevisiae</i>

Dickinson, J. Richard; Norte, Valia A.

doi:10.1016/0014-5793(93)81754-n

Cited by 58 publications

(38 citation statements)

References 22 publications

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“…Growth in the presence of fusel alcohols such as isoamyl alcohol (derived from leucine) induces pseudohyphal growth (9,33). These pronounced morphological modifications are accompanied by an increase in the specific activity of succinate dehydrogenase and an increase of the chitin content (33).…”

Section: Fusel Alcohols and Acids As Signal Molecules: Role Of The Ehmentioning

confidence: 99%

The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism

Hazelwood

Daran

Maris

et al. 2008

Appl Environ Microbiol

1,210

613

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Section: Fusel Alcohols and Acids As Signal Molecules: Role Of The Ehmentioning

confidence: 99%

The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism

Hazelwood

Daran

Maris

et al. 2008

Appl Environ Microbiol

1,210

613

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“…In S. cerevisiae, the first two steps of branchedchain amino acid catabolism are similar to those in other eukaryotes in that they involve first an aminotransferase and then 2-oxoacid dehydrogenase (Dickinson & Norte, 1993;Dickinson & Dawes, 1992). However, in yeast, major products of branched-chain amino acid catabolism are the so-called 'fusel' alcohols (Webb & 1963).…”

Section: Resultsmentioning

confidence: 99%

Fuse1 alcohols induce hyphal-like extensions and pseudohyphal formationin yeasts

1996

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At a concentration of 0*5% (vlv), isoamyl alcohol induced the formation of hyphal-like extensions in haploid and diploid strains of Saccharomyces cerewisiae in liquid complex medium. These extensions, which develop via bud initiation and elongation, undergo DNA replication and nuclear division and appear similar in many respects to an aberrant form of the cell division cycle. However, in 0 2 5 O/ O (vh) isoamyl alcohol, 5. cerewisiae formed pseudohyphae. Other 'fusel' alcohols (which are the products of amino acid catabolism) also induced hyphal-like extensions in this yeast, with n-amyl alcohol being as equally effective as isoamyl alcohol. lsoamyl alcohol induced the formation of pseudohyphae in two species of Candida and both hyphal-like extensions and pseudohyphae in Brettanomyces anomalus, suggesting a close relationship or a common basis to the development of the two morphologies.

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“…Some metabolic schemes even envisage a blending of catabolic and biosynthetic pathways (8). These and other considerations have led us to undertake a thorough re-examination of the catabolism of the branched-chain amino acids in S. cerevisiae which has confirmed that the first step is transamination (9). Two distinct aminotransferases have been shown to function both in amino acid biosynthesis and catabolism.…”

mentioning

confidence: 99%

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae

Dickinson¹,

Harrison²,

Hewlins³

1998

Journal of Biological Chemistry

166

136

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The metabolism of valine to isobutyl alcohol in yeast was examined by 13 C nuclear magnetic resonance spectroscopy and combined gas chromatography-mass spectrometry. The product of valine transamination, ␣-ketoisovalerate, had four potential routes to isobutyl alcohol. The first, via branched-chain ␣-ketoacid dehydrogenase to isobutyryl-CoA is not required for the synthesis of isobutyl alcohol because abolition of branchedchain ␣-ketoacid dehydrogenase activity in an lpd1 disruption mutant did not prevent the formation of isobutyl alcohol. The second route, via pyruvate decarboxylase, is the one that is used because elimination of pyruvate decarboxylase activity in a pdc1 pdc5 pdc6 triple mutant virtually abolished isobutyl alcohol production. A third potential route involved ␣-ketoisovalerate reductase, but this had no role in the formation of isobutyl alcohol from ␣-hydroxyisovalerate because cell homogenates could not convert ␣-hydroxyisovalerate to isobutyl alcohol. The final possibility, use of the pyruvate decarboxylase-like enzyme encoded by YDL080c, seemed to be irrelevant, because a strain with a disruption in this gene produced wild-type levels of isobutyl alcohol. Thus there are major differences in the catabolism of leucine and valine to their respective "fusel" alcohols. Whereas in the catabolism of leucine to isoamyl alcohol the major route is via the decarboxylase encoded by YDL080c, any single isozyme of pyruvate decarboxylase is sufficient for the formation of isobutyl alcohol from valine. Finally, analysis of the 13 C-labeled products revealed that the pathways of valine catabolism and leucine biosynthesis share a common pool of ␣-ketoisovalerate.

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A study of branched‐chain amino acid aminotransferase and isolation of mutations affecting the catabolism of branched‐chain amino acids in Saccharomyces cerevisiae

Cited by 58 publications

References 22 publications

The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism

The Ehrlich Pathway for Fusel Alcohol Production: a Century of Research on Saccharomyces cerevisiae Metabolism

Fuse1 alcohols induce hyphal-like extensions and pseudohyphal formationin yeasts

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae

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