Trickle-bed air biofilters (TBABs) are suitable for treating volatile organic compounds (VOCs) at a significantly high practical loading because of their controlled environmental conditions. The application of TBAB for treating styrene-contaminated air under periodic backwashing and cyclical nonuse periods at a styrene loading of 0.64 -3.17 kg chemical oxygen demand (COD)/m 3 ⅐day was the main focus of this study. Consistent long-term efficient performance of TBAB strongly depended on biomass control. A periodic in situ upflow with nutrient solution under media fluidization, that is, backwashing, was approached in this study. Two different nonuse periods were employed to simulate a shutdown for equipment repair or during weekends and holidays. The first is a starvation period without styrene loading, and the second is a stagnant period, which reflects no flow passing through the biofilter.For styrene loadings up to 1.9 kg COD/m 3 ⅐day, removal efficiencies consistently above 99% were achieved by conducting a coordinated biomass control strategy, that is, backwashing for 1 hr once per week. Under cyclical nonuse periods for styrene loadings up to 1.27 kg COD/m 3 ⅐day, stable long-term performance of the biofilter was maintained at more than 99% removal without employing backwashing. No substantial impact of nonuse periods on the biofilter performance was revealed. However, a coordinated biomass control by backwashing subsequently was unavoidable for attaining consistently high removal efficiency at a styrene loading of 3.17 kg COD/m 3 ⅐day.As styrene loading was increased, reacclimation of the biofilter to reach the 99% removal efficiency following backwashing or the nonuse periods was delayed. After the nonuse periods, the response of the biofilter was a strong function of the biomass in the bed. No significant difference between the effects of the two different nonuse periods on TBAB performance was observed during the study period.