A Study of Conditions for Kjeldahl Determination of Nitrogen in Proteins

Hiller, Alma; Plazin, John; Slyke, Donald D. Van

doi:10.1016/s0021-9258(18)57154-4

Cited by 343 publications

(24 citation statements)

References 29 publications

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“…An aliquot of each solution was then diluted in 0.1 N NaOH and an absorption spectrum obtained in the Beckman spectrophotometer, model DU (11). On other aliquots, in duplicate, nitrogen was determined by micro-Kjeldahl analysis (12). Samples of bovine gamma globulin were treated in an identical manner, save for omission of D compound, thereby prodding control material.…”

mentioning

confidence: 99%

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

Eisen

Orris

Belman

1952

Journal of Experimental Medicine

176

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Allergic skin reactions of the delayed type may be conveniently considered to involve two distinct processes: first, induction of the sensitized state, and second, elicitation of the allergic reaction in the sensitized individual. With regard to the induction of sensitization, it is generally believed that low molecular weight substances (haptens) can function effectively in this respect if they combine in vivo with tissue constituents, presumably proteins. This belief, which had long been reasonably maintained on the basis of analogy with the conditions required for the production of antisera by haptens, achieved a high degree of plausibility as a result of the systematic studies of Landsteiner and Jacobs (1, 2).The question as to whether hapten combination with protein is involved in the second process, namely, elicitation of the reaction, has received little attention. In many studies of the elicitation process, haptens which are homologous in structure to the hapten used for inducing sensitization often have produced widely divergent reactions: some homologues have elicited skin lesions, while other have not (3)(4)(5). This diversity of responses has been interpreted quite consistently in terms of specificity arising from the configurational conformity of the elicifing compound to the sensitizing hapten, little attention being paid to other factors that might modify the responses. However, from a consideration of these earlier studies, it appeared possible that, in addition to configurational features, at least one other factor--namely, combination of the eliciting hapten with protein--might be involved in accounting for the diverse responses sometimes obtained in eliciting reactions with haptens which are structurally similar to the substance used for inducing sensitization. Accordingly, we have sought to determine with a group of 2,4-dinitrophenyl compounds whether combination with protein is important in determining the effectiveness of haptens in producing delayed allergic skin lesions in sensitized guinea pigs and men.

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confidence: 99%

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

Eisen

Orris

Belman

1952

Journal of Experimental Medicine

176

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“…Non-protein nitrogen (NPN) was determined on 10-ml. samples of the extract, using the micro-Kjeldahl digestion method of Hiller et al (1948) and distilling the digested mixture into 0.1 N HC1 in an all-glass still. Ammonia nitrogen (NH 3 N) was separated by distilling the undigested extract in the same still.…”

Section: Methodsmentioning

confidence: 99%

The Hormonal Control of Metabolism in Crustaceans. Ix. Carbohydrate Metabolism in the Transition From Intermoult to Premoult in Carcinides Maenas

Scheer

1959

The Biological Bulletin

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“…Protein content was measured by estimating the N content and then multiplying the N value by 6.25 [24]. Nutrient (N, P, K, S, Zn and B) uptake by the test crop was calculated from the results of crop yield and nutrient content in seed and straw [25].…”

Section: Protein Content and Nutrient Uptake Determinationmentioning

confidence: 99%

Assessment of Potassium Element on Lentil (<i>Lens culinaris</i> Medic) Agronomy and Nutrient Use Efficiency in Calcareous Soils

Quddus¹,

Hossain²,

Naser³

et al. 2019

JENR

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Potassium (K) is considered as key element for plant growth and physiology and improvement of productivity and quality of crops. The potassium (K) reduction from agricultural soils of Indo-Gangetic plain had been observed due to rapidly increasing intensive cropping systems, in combination with imbalanced fertilization. Now, burning issue is needed to better understand the role and dynamic of K in plants and K-use efficiency of crops. Hence, this study was undertaken during two consecutive years of 2015-16 and 2016-17 to know the effects of different levels of potassium on lentil productivity, nodulation, nutrient uptake and K use efficiency. The experiment was laid out in randomized complete block design considering five treatments with thrice replicates. The treatments were T 1 (K Control), T 2 (15 kg K ha -1 ), T 3 (30 kg K ha -1 ), T 4 (45 kg K ha -1 ) and T 5 (60 kg K ha -1 ) along with the blanket dose of N 18 P 20 S 10 Zn 2 B 1.5 kg ha -1 and cowdung 3 t ha -1 . Yield and yield attributes of lentil responded significantly to K fertilizer. The highest mean seed yield (1684 kg ha -1 ) and maximum seed yield increase over control (31.9%) got from T 5 treatment followed by T 4 treatment. The highest total nutrient (N, P, K, S, Zn and B) uptake, maximum nodulation and the highest protein content (29.4%) in seed was, however, recorded from the plot receiving of 60 kg K ha -1 . Apparent K recovery efficiency was recorded higher (17.8%) from T 4 treatment. Apparent K recovery efficiency followed the order: T 4 >T 5 >T 3 >T 2 . From the economic point of view, T 5 followed by T 4 treatment is viable and sound. The results of the experiment suggest that 60 kg K ha -1 might be applied along with N 18 P 20 S 10 Zn 2 B 1.5 kg ha -1 for lentil cultivation in calcareous soils of Bangladesh. The K rates for fertilizer recommendation in lentil need to be revised to take account for highest yield by higher increasing rate of K in soil.

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A Study of Conditions for Kjeldahl Determination of Nitrogen in Proteins

Cited by 343 publications

References 29 publications

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

The Hormonal Control of Metabolism in Crustaceans. Ix. Carbohydrate Metabolism in the Transition From Intermoult to Premoult in Carcinides Maenas

Assessment of Potassium Element on Lentil (<i>Lens culinaris</i> Medic) Agronomy and Nutrient Use Efficiency in Calcareous Soils

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A Study of Conditions for Kjeldahl Determination of Nitrogen in Proteins

Cited by 343 publications

References 29 publications

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

Elicitation of Delayed Allergic Skin Reactions With Haptens: The Dependence of Elicitation on Hapten Combination With Protein

The Hormonal Control of Metabolism in Crustaceans. Ix. Carbohydrate Metabolism in the Transition From Intermoult to Premoult in Carcinides Maenas

Assessment of Potassium Element on Lentil (&lt;i&gt;Lens culinaris&lt;/i&gt; Medic) Agronomy and Nutrient Use Efficiency in Calcareous Soils

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Assessment of Potassium Element on Lentil (<i>Lens culinaris</i> Medic) Agronomy and Nutrient Use Efficiency in Calcareous Soils