A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy

Morris, James H.; Hudson, Alan R.; Weddell, G.

doi:10.1007/bf00335678

Cited by 165 publications

(98 citation statements)

References 87 publications

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“…The elevated number within the graft can be attributed to increased collateral sprouting within the graft. 23–25 Interestingly, the mid-graft fiber counts are similar in magnitude to the fiber counts of the cut and repair group. This observation provides further support for the premise that a portion of regenerating nerve fibers fail to successfully navigate across sutured repairs.…”

Section: Discussionmentioning

confidence: 65%

“…Transected peripheral nerves typically regenerate with multiple axon sprouts arising from each nerve fiber. 23–25 It is possible that this initial burst of regeneration (and the subsequent decline) was not captured during the time points studied. Another possibility is that the process of sprouting and pruning is more dynamic than previously recognized, with some nerves sprouting as others are pruned.…”

Section: Discussionmentioning

confidence: 99%

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Axonal regeneration and motor neuron survival after microsurgical nerve reconstruction

et al. 2012

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Rodent models are used extensively for studying nerve regeneration, but little is known about how sprouting and pruning influence peripheral nerve fiber counts and motor neuron pools. The purpose of this study was to identify fluctuations in nerve regeneration and neuronal survival over time. One hundred and forty-four Lewis rats were randomized to end-to-end repair or nerve grafting (1.5 cm graft) after sciatic nerve transection. Quantitative histomorphometry and retrograde labeling of motor neurons were performed at 1, 3, 6, 9, 12, and 24 months and supplemented by electron microscopy. Fiber counts and motor neuron counts increased between 1 and 3 months, followed by plateau. End-to-end repair resulted in persistently higher fiber counts compared to the grafting for all time points (P < 0.05). Percent neural tissue and myelin width increased with time while fibrin debris dissipated. In conclusion, these data detail the natural history of regeneration and demonstrate that overall fiber counts may remain stable despite pruning.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

Axonal regeneration and motor neuron survival after microsurgical nerve reconstruction

et al. 2012

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“…Since it is not generally agreed which type of evaluation tool is the most useful for evaluation of functional recovery; our research group like others has been using different methods for an overall assessment of nerve function, which has been widely recommended [53] . The sciatic nerve regeneration after neurotmesis studies described by the authors were followed during 20 wk after the surgical procedure based on the previous experimental work [17,29,54,55] .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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“…Early during the regeneration process axons arborize and send multiple axons into different endoneurial tubes and pathways. Over time, axonal sprouts that have grown into inappropriate pathways are pruned (Pruning Hypothesis); whereas, those traversing more appropriate pathways are conserved (Morris, et al, 1972, Witzel, et al, 2005). For the second mechanism, proteases produced by macrophages, Schwann cells, and fibroblasts are thought to make the basal lamina more porous allowing growth cones to change direction or grow less restricted between distal endoneurial tubes (Fu and Gordon, 1997, Ide, 1996).…”

Section: Discussionmentioning

confidence: 99%

Sensory axon targeting is increased by NGF gene therapy within the lesioned adult femoral nerve

Cai

Yang

et al. 2010

Experimental Neurology

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Even though peripheral nerves regenerate well, axons are often misrouted and reinnervate inappropriate distal pathways post-injury. Misrouting most likely occurs at branch points where regenerating axons make choices. Here, we show that the accuracy of sensory axon reinnervation is enhanced by overexpression of the guidance molecule nerve growth factor (NGF) distal to the bifurcation. We used the femoral nerve as model, which contains both sensory and motor axons that intermingle in the parent trunk and distally segregate into the saphenous (SB) and motor branch (MB). Transection of the parent trunk resulted in misrouting of axon reinnervation to SB and MB. To enhance sensory axon targeting, recombinant adenovirus encoding NGF was injected along the SB close to the bifurcation one week post-injury. The accuracy of axon reinnervation was assessed by retrograde tracing at 3 or 8 weeks after nerve injury. NGF overexpression significantly increased the accuracy of SB axon reinnervation to the appropriate nerve branch, in a manner independent of enhancing axon regeneration. This novel finding provides in vivo evidence that gradient expression of neurotrophin can be used to enhance targeting of distal peripheral pathways to increase axon regeneration into the appropriate nerve branch.

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A study of degeneration and regeneration in the divided rat sciatic nerve based on electron microscopy

Cited by 165 publications

References 87 publications

Axonal regeneration and motor neuron survival after microsurgical nerve reconstruction

Axonal regeneration and motor neuron survival after microsurgical nerve reconstruction

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Sensory axon targeting is increased by NGF gene therapy within the lesioned adult femoral nerve

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