A Study of Degradation Mechanism of Alginate by Gamma-irradiation

Luân, Lê Quang; Nagasawa, Naotsugu; Ha, Vo Thi Thu; Nakanishi, Teruyuki

doi:10.3769/radioisotopes.58.1

Cited by 18 publications

(5 citation statements)

References 28 publications

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“…Figure 2 depicts the UV spectra of the irradiated and unirradiated forms of sodium alginate; it reveals the formation of a new absorption band at approximately 265 nm in ISA, which was absent in UISA. The formation of the new peak could be attributed to the formation of carbonyl (C=O) groups after the main chain scission of alginate and hydrogen abstraction, followed by the ring opening in the radiation-induced degradation process (Luan et al, 2009;El-Mohdy, 2012). 3.2.…”

Section: Resultsmentioning

confidence: 99%

Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Shabbir¹,

Khan²,

Sadiq³

et al. 2017

Turk J Biol

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Upon irradiation, marine polysaccharides undergo depolymerization, leading to formation of oligosaccharides that elicit various biological activities in plants. Taking a step further on the previously established growth-promoting activity of irradiated sodium alginate (ISA), structural rearrangements in ISA were analyzed using complementary techniques to develop an understanding of the structure-property relationship. The essential oil (EO) of vetiver (Vetiveria zizanioides L. Nash) immensely benefits the perfumery industry, proving itself as an economically important crop. A pot experiment was designed to test the effect of water-soluble ISA fractions on the growth, physiology, and EO production of vetiver. The structural characterization of radiation-induced sodium alginate was carried out using SEM, FT-IR, and UV-Vis spectroscopy. Of the various treatments employed, ISA-120 (ISA applied at 120 mg L -1 ) proved the best for most of the parameters studied, including fresh and dry weight of plants and photosynthetic attributes. As compared to the control (water-spray treatment), foliar feeding of ISA-120 resulted in an increase of 21.2% in chlorophyll content, while this treatment enhanced the chlorophyll fluorescence by 21.8% at 300 days after transplanting. Application of ISA-120 also increased the EO content by 21.1% and EO yield by 47.6%. Gas chromatography revealed an increase of 30.1% and 92.6% in the values of content and yield of khusimol, respectively, over the control.

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Section: Resultsmentioning

confidence: 99%

Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Shabbir¹,

Khan²,

Sadiq³

et al. 2017

Turk J Biol

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“…Consistent with our hypothesis, Falkeborg et al ( 2014 ) and Huang et al ( 2021 ) depolymerized the complex polysaccharide alginate into oligosaccharides using alginate lyase, which displayed better antioxidant activity due to the presence of the conjugated alkene acid structure formed during enzymatic depolymerization. Luan et al ( 2009 ) and Chang et al ( 2022 ) also used radiation to depolymerize alginate and proposed that the general process of radiation-chemical transformation causes glycosidic bond disintegration. Carbonyl groups and double bonds between the C-4 and C-5 residues are formed during this process.…”

Section: Discussionmentioning

confidence: 99%

Seaweed Sargassum aquifolium extract ameliorates cardiotoxicity induced by doxorubicin in rats

Samir

Hassan

Saber

et al. 2023

Environ Sci Pollut Res

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Doxorubicin (DOX) is a potent anticancer drug with adverse cardiotoxic effects. Alginates are multifunctional biopolymers and polyelectrolytes derived from the cell walls of brown seaweeds. They are nontoxic, biocompatible, and biodegradable, and hence, utilized in several biomedical and pharmaceutical applications. Here, we investigated the potential cardioprotective effect of thermally treated sodium alginate (TTSA), which was extracted and purified from the seaweed Sargassum aquifolium, in treating acute DOX cardiotoxicity and apoptotic pathways in rats. UV–visible spectroscopy, Fourier-transform infrared, and nuclear magnetic resonance (1H–NMR) spectroscopy techniques were used to characterize TTSA. CK-MB and AST levels in sera samples were determined. The expression levels of Erk-2 (MAPK-1) and iNOS genes were investigated by quantitative real-time polymerase chain reaction (qRT-PCR). The protein expression levels of Erk-2, anti-apoptotic p53, and caspase-3 were analyzed using western blotting and ELISA. For the in vivo studies, sixty rats were randomly divided equally into six groups and treated with DOX, followed by TTSA. We revealed that treatment with TTSA, which has low molecular weight and enhanced antioxidant properties, improved DOX-mediated cardiac dysfunction and alleviated DOX-induced myocardial apoptosis. Furthermore, TTSA exhibited a cardioprotective effect against DOX-induced cardiac toxicity, indicated by the increased expression of MAPK-1 (Erk2) and iNOS genes, which are implicated in the adaptive responses regulating DOX-induced myocardial damage. Moreover, TTSA significantly (p < 0.05) suppressed caspase-3 and upregulated anti-apoptotic protein p53 expression. TTSA also rebalanced the cardiomyocyte redox potential by significantly (p < 0.05) increasing the levels of endogenous antioxidant enzymes, including catalase and superoxide dismutase. Our findings suggest that TTSA, particularly at a dose of 400 mg/kg b.w., is a potential prophylactic supplement for treating acute DOX-linked cardiotoxicity.

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“…This absorption peak is ascribed to the n / p* transition of carbonyl groups (C]O) and corresponds to alginate degraded by gamma irradiation. 12 This indicates that articial solar irradiation of TiO 2 /C anodes enhances alginate oxidation, and results in the generation of C]O bonds. In previous reports concerning alginate degradation, two oxidation pathways have been demonstrated.…”

Section: Analysis Of the Fuel Cell Reaction Productsmentioning

confidence: 97%

“…The potential applications of alginate, a polysaccharide comprising a large fraction of the structural makeup of macroalgae, have prompted researchers to attempt its degradation, for example, by enzymatic treatment with engineered microbes, acid treatment, and gamma irradiation. [8][9][10][11][12] However, these methods are expensive and energy intensive, which renders biofuel production from alginate impractical. We have previously attempted to oxidize alginate using a fuel cell setup with anodes modied with carbon nanotubes and gold nanoparticles, the latter of which exhibits catalytic activity towards glucose oxidation under alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

Photocatalytic alginate fuel cells for energy production and refining of macroalgae

et al. 2017

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A Study of Degradation Mechanism of Alginate by Gamma-irradiation

Cited by 18 publications

References 28 publications

Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Regulation of functional activities and essential oil production in Vetiveria zizanioides L. Nash after γ-irradiated sodium alginate elicitation

Seaweed Sargassum aquifolium extract ameliorates cardiotoxicity induced by doxorubicin in rats

Photocatalytic alginate fuel cells for energy production and refining of macroalgae

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