A study of high frequency ultrasound scattering from non-nucleated biological specimens

Falou, Omar; Baddour, Ralph E.; Nathanael, George; Czarnota, Gregory J.; Kumaradas, J. Carl; Kolios, Michael C.; Church, Charles C.

doi:10.1121/1.2987462

Cited by 17 publications

(13 citation statements)

References 10 publications

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“…These values are required as input to theoretical models of scattering developed in our laboratory [15]. Finally, the acoustic microscope can be used in the study of molecular imaging and the dynamics of how ultrasound contrast agents interact with sound-fields when attached to a cell.…”

Section: Discussionmentioning

confidence: 99%

Measuring the mechanical properties of cells using acoustic microscopy

Strohm

Kolios

2009

2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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Time resolved acoustic microscopy was used to investigate the mechanical properties of MCF7 breast cancer cells at 375 MHz. The thickness, speed of sound, acoustic impedance, density, bulk modulus and attenuation were calculated using the amplitude and time of the ultrasound backscatter from the cellular membrane and substrate. The technique was first validated by measuring the mechanical properties of a material with well characterized properties, polyvinylidene fluoride (PVDF). The measured values agreed very well (within 4.5%) with those provided from the manufacturer. The same properties of MCF7 cells were then measured using the same technique. The speed of sound, acoustic impedance, density and bulk modulus were found to have values slightly higher than the medium they were testing in. In this paper, a technique to non-invasively measure the mechanical properties of cells using scanning acoustic microscopy is presented.

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Section: Discussionmentioning

confidence: 99%

Measuring the mechanical properties of cells using acoustic microscopy

Strohm

Kolios

2009

2009 Annual International Conference of the IEEE Engineering in Medicine and Biology Society

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“…This has been previously done by scanning a sample with objects in suspension and analyzing the brightest signals received, assuming these to be caused by scattering events from the single objects of interest ͑Baddour et Baddour and Kolios, 2007;Falou et al, 2008͒. This technique is error-prone since the bright signals may be from unwanted particles in the suspension, aggregates of the objects of interest ͑e.g., a cluster of cells͒, or from air bubbles, which are hard to eliminate from fluid suspensions.…”

Section: Introductionmentioning

confidence: 98%

The measurement of ultrasound scattering from individual micron-sized objects and its application in single cell scattering

Falou

Min

Kaffas

et al. 2010

The Journal of the Acoustical Society of America

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The measurement of the ultrasound backscatter from individual micron-sized objects such as cells is required for various applications such as tissue characterization. However, performing such a measurement remains a challenge. For example, the presence of air bubbles in a suspension of cells during the measurements may lead to the incorrect interpretation of the acoustic signals. This work introduces a technique for measuring the ultrasound backscatter from individual micron-sized objects by combining a microinjection system with a co-registered optical microscope and an ultrasound imaging device. This allowed the measurement of the ultrasound backscatter response from a single object under optical microscope guidance. The optical and ultrasonic data were used to determine the size of the object and to deduce its backscatter responses, respectively. In order to calibrate the system, the backscatter frequency responses from polystyrene microspheres were measured and compared to theoretical predictions. A very good agreement was found between the measured backscatter responses of individual microspheres and theoretical predictions of an elastic sphere. The backscatter responses from single OCI-AML-5 cells were also investigated. It was found that the backscatter responses from AML cells are best modeled using the fluid sphere model. The advantages, limitations, and future applications of the developed technique are discussed.

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“…Because of its complex structures, not all cells can be exactly approximated by simple fluid sphere models including the T-matrix and Anderson’s model [26]. Good agreement between theoretical and experimental backscattered data, however, has been demonstrated by Anderson’s model, for cells like OCI-AML-5, PC-3 prostate carcinoma cells [27], and sea urchin oocytes [28], whose nuclei are relatively small compared with their cell bodies.…”

Section: Resultsmentioning

confidence: 99%

Backscattering measurement from a single microdroplet

Lee

Chang

Jeong

et al. 2011

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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Backscattering measurements for acoustically trapped lipid droplets were undertaken by employing a P[VDF-TrFE] broadband transducer of f-number = 1, with a bandwidth of 112%. The wide bandwidth allowed the transmission of the 45 MHz trapping signal and the 15 MHz sensing signal using the same transducer. Tone bursts at 45 MHz were first transmitted by the transducer to hold a single droplet at the focus (or the center of the trap) and separate it from its neighboring droplets by translating the transducer perpendicularly to the beam axis. Subsequently, 15 MHz probing pulses were sent to the trapped droplet and the backscattered RF echo signal received by the same transducer. The measured beam width at 15 MHz was measured to be 120 μm. The integrated backscatter (IB) coefficient of an individual droplet was determined within the 6-dB bandwidth of the transmit pulse by normalizing the power spectrum of the RF signal to the reference spectrum obtained from a flat reflector. The mean IB coefficient for droplets with a 64 μm average diameter (denoted as cluster A) was −107 dB, whereas it was −93 dB for 90-μm droplets (cluster B). The standard deviation was 0.9 dB for each cluster. The experimental values were then compared with those computed with the T-matrix method and a good agreement was found: the difference was as small as 1 dB for both clusters. These results suggest that this approach might be useful as a means for measuring ultrasonic backscattering from a single microparticle, and illustrate the potential of acoustic sensing for cell sorting.

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A study of high frequency ultrasound scattering from non-nucleated biological specimens

Cited by 17 publications

References 10 publications

Measuring the mechanical properties of cells using acoustic microscopy

Measuring the mechanical properties of cells using acoustic microscopy

The measurement of ultrasound scattering from individual micron-sized objects and its application in single cell scattering

Backscattering measurement from a single microdroplet

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