“…The present novel insights indicate that a countermeasure exercise programme performed onboard the ISS generally preserves passive stiffness in muscle structures at most sites measured (shoulder, neck, back, thigh), as changes inflight showed only marginal differences from pre- and postflight measurements. Importantly, the Tibialis Anterior muscle (prime dorsiflexor, ankle joint stabilizer 44 , vital in human gait on Earth, showed reduced stiffness inflight in all 12 mission crew members studied in spite of routine inflight countermeasures currently available on the ISS 45 (T2, treadmill-2; ARED, advanced resistive exercise device; CEVIS, bicycle ergometer). This selective lack of preservation of stiffness, suggesting muscle loss/weakness, in an important muscle for gait is a new and unexpected finding, which requires consideration for ISS astronauts and longer-duration missions to Deep Space.…”