A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species

Pavlović, Mariana Oalđe; Kolarević, Stoimir; Đorđević, Jelena; Marić, Jovana Jovanović; Lunić, Tanja; Mandić, Marija; Kračun-Kolarević, Margareta; Živković, Jelena; Aradski, Ana Alimpić; Marin, Petar D.; Šavikin, Katarina; Vuković-Gačić, Branka; Nedeljković, Biljana Božić; Duletić-Laušević, Sonja

doi:10.3390/plants10112306

Cited by 17 publications

(13 citation statements)

References 68 publications

(95 reference statements)

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“…Taken together, the results from TPC, DPPH and ORAC assays suggest that leaf ethanol extracts have more potent antioxidant bioactivity potential than stem extracts. This difference in biological properties between the subparts of the plant and as a function of the extraction procedure have already been observed by other research groups in both basil (between leaves and flowers) [ 49 ] and other vegetables [ 50 ]. The phytochemical detected in both parts of the plant are known for their antioxidant potential.…”

Section: Discussionsupporting

confidence: 61%

“…This anti-oncogenesis property and associated genoprotective effects would be mainly correlated with basil impact on oxidative stress and NO modulation rather than TNF. Therefore it might be suggested that the absence of variation of the latter cytokine in our assay, for both leaves and stems, might be considered as in accordance rather than as a discrepancy regarding the biological potential of Ocimum basilicum [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

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Differential Nutrition-Health Properties of Ocimum basilicum Leaf and Stem Extracts

Bensaid¹,

Boudard²,

Servent³

et al. 2022

Foods

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(1) Background: Ocimum basilicum L. is an aromatic medicinal plant of the Lamiaceae family known as sweet basil. It is used in traditional medicine for its beneficial effects on gastrointestinal disorders, inflammation, immune system, pyrexia or cancer among others. Ocimum basilicum (OB) leaf extracts contain many phytochemicals bearing the plant health effects but no reports is available on the potential bioactivity of stem extracts. Our investigation aimed at assessing the differential biological activity between basil leaf and stem to promote this co-product valorization. (2) Method: For this purpose we explored phytochemical composition of both parts of the plant. Antioxidant activity was evaluated through total polyphenol content measure, DPPH and ORAC tests. Anti-inflammatory markers on stimulated macrophages, including NO (nitric oxide), TNFa (tumor necrosis factor alpha), IL-6 (interleukin 6), MCP1 (monocyte attractant protein 1) and PGE-2 (prostaglandin E2), were evaluated. In addition, we investigated OB effects on jejunum smooth muscle contractility. (3) Results: OB extracts from leaves and stems demonstrated a different biological activity profile at the level of both antioxidant, anti-inflammatory and smooth muscle relaxation effects. (4) Conclusion: Taken together our results suggest that Ocimum basilicum extracts from co-product stems, in addition to leaves, may be of interest at the nutrition-health level with specific therapeutic potential.

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Section: Discussionsupporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

Differential Nutrition-Health Properties of Ocimum basilicum Leaf and Stem Extracts

Bensaid¹,

Boudard²,

Servent³

et al. 2022

Foods

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“…At high concentrations, polyphenolic compounds exhibit pro-oxidant and cytotoxic properties. At the same concentrations in tumor and normal cells, polyphenols can induce oppositely directed effects ( Oalđe Pavlović et al, 2021 ).…”

Section: Chemosensitization Of Tumors With Polyphenolsmentioning

confidence: 99%

“…Resveratrol Human herpesvirus 1 and Human herpesvirus 2 ND Significantly reduced skin lesions, and the effectiveness of the drug depended on its concentration, the time of initiation of treatment and the number of applications per day (Docherty et al, 2004), (Docherty et al, 2005) Epigallocatechin Pavlović et al, 2021). EGCG inhibits ROS generation in normal epithelial cells but induces ROS generation in tumor cells.…”

Section: Mechanism Of Action Referencesmentioning

confidence: 99%

Modern aspects of the use of natural polyphenols in tumor prevention and therapy

Суфианова

Gareev

Beylerli

et al. 2022

Front. Cell Dev. Biol.

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Polyphenols are secondary plant metabolites or organic compounds synthesized by them. In other words, these are molecules that are found in plants. Due to the wide variety of polyphenols and the plants in which they are found, these compounds are divided according to the source of origin, the function of the polyphenols, and their chemical structure; where the main ones are flavonoids. All the beneficial properties of polyphenols have not yet been studied, since this group of substances is very extensive and diverse. However, most polyphenols are known to be powerful antioxidants and have anti-inflammatory effects. Polyphenols help fight cell damage caused by free radicals and immune system components. In particular, polyphenols are credited with a preventive effect that helps protect the body from certain forms of cancer. The onset and progression of tumors may be related directly to oxidative stress, or inflammation. These processes can increase the amount of DNA damage and lead to loss of control over cell division. A number of studies have shown that oxidative stress uncontrolled by antioxidants or an uncontrolled and prolonged inflammatory process increases the risk of developing sarcoma, melanoma, and breast, lung, liver, and prostate cancer. Therefore, a more in-depth study of the effect of polyphenolic compounds on certain signaling pathways that determine the complex cascade of oncogenesis is a promising direction in the search for new methods for the prevention and treatment of tumors.

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“…Ultrasound-assisted extraction, microwave-assisted extraction, and supercritical fluid extraction belong to a group of novel extraction methods (Jovanović et al, 2017a). The ultrasoundassisted extraction (UAE) has previously been employed by Oal de Pavlović et al (2021), in order to obtain S. montana extracts for further determination of their biological activities, such as antioxidant capacity, antitumor potential and genoprotective activity in acellular system. Ultrasound waves are high-frequency sound waves that are above the human hearing threshold or 20 kHz.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical properties of winter savory extracts prepared using ultrasound-assisted extraction

Čutović¹,

Batinić²,

Marković³

et al. 2022

Lekovite sirovine

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This study aimed to assess the optimal extraction parameters, solvent-to-solid ratio, and extraction time, for ultrasound-assisted extraction of Satureja montana L. (winter savory) harvested from a 6-year-old experimental plantation. The optimization was performed in order to acquire extracts that are rich in the content of polyphenols (TPC), flavonoids (TFC), and proteins, and also exhibit strong antioxidant activity, while maintaining satisfactory physical characteristics. Besides this, the influence of UV irradiation on the physicochemical properties of the selected extracts was also analyzed. The TPC values increased with longer extraction time and higher ratio (ranging from 41.4 to 56.9 mg gallic acid equivalent (GAE)/g for the ratio 30:1, and 52.4-58.4 mg GAE/g for 40:1), and the TFC followed the same trend. Total protein content values increased with the increase in the solvent-to-solid ratio, while the extraction time had no significant influence. All used antioxidant assays showed better activities when a higher solvent-to-solid ratio has been performed. pH values ranged from 6.00 to 6.46. The extraction time and solvent-to-solid ratio did not significantly influence the conductivity, whereas the zeta potential was significantly affected. The highest density was detected in the extract acquired at a 40:1 ratio after 30 min (0.994 g/mL), and higher viscosity of the extracts attained at a 30:1 ratio compared to the parallels at a 40:1 ratio. According to the results of UV stability, UV light significantly affected the TPC, TFC, total proteins, ABTS radical scavenging, cupric, and ferric ion-reducing activities, with a positive effect on ferric ion-reducing potential, and the denisty. In conclusion, the extraction conditions that yielded the best phenolic-rich extract with good antioxidant potential and satisfactory physical properties were a 40:1 solvent-to-solid ratio and a 30 minute extraction time.

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A Study of Phytochemistry, Genoprotective Activity, and Antitumor Effects of Extracts of the Selected Lamiaceae Species

Cited by 17 publications

References 68 publications

Differential Nutrition-Health Properties of Ocimum basilicum Leaf and Stem Extracts

Differential Nutrition-Health Properties of Ocimum basilicum Leaf and Stem Extracts

Modern aspects of the use of natural polyphenols in tumor prevention and therapy

Physicochemical properties of winter savory extracts prepared using ultrasound-assisted extraction

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