A study of structural differences between liver cancer cells and normal liver cells using FTIR spectroscopy

Sheng, Daping; Xu, Fang; Yu, Qiang; Fang, Tingting; Xia, Junjun; Li, Se-Ruo; Wang, Xin

doi:10.1016/j.molstruc.2015.05.054

Cited by 37 publications

(16 citation statements)

References 30 publications

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“…FT-IR spectroscopy could be used to look at the secondary structures of proteins, in fact the 20 structures of the whole molecules or proteins may be determined from the analysis of amide I band of proteins that occurs due to the in-plane C=O stretching vibrations which are weakly coupled to the C-N stretching vibrations and in-plane N-H bending vibrations. 31 As shown in the reference figure, the peak location, intensity, and shape change greatly. Hydroxy absorption peak from protein, Amide A band (3290 cm −1 ) is almost absent in the liver of DMH exposed group, no obvious difference in intensity of this peak has been observed in other groups.…”

Section: Discussionmentioning

confidence: 94%

“…The FT‐IR spectra revealed dramatic differences in the band positions and bandwidths, signal intensity values and signal intensity ratios between the CP‐treated group and the group which received both CP and EA. FT‐IR spectroscopy could be used to look at the secondary structures of proteins, in fact the 20 structures of the whole molecules or proteins may be determined from the analysis of amide I band of proteins that occurs due to the in‐plane C=O stretching vibrations which are weakly coupled to the C‐N stretching vibrations and in‐plane N‐H bending vibrations . As shown in the reference figure, the peak location, intensity, and shape change greatly.…”

Section: Discussionmentioning

confidence: 99%

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Ellagic acid ameliorates cisplatin induced hepatotoxicity in colon carcinogenesis

Goyal

Koul

Ranawat

2019

Environmental Toxicology

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The clinical application of cisplatin (CP), one of the most extensively used antineoplastic drug, is restricted by its numerous side effects. CP's antitumor potential resides in the free generation of reactive oxygen species leading to oxidative stress. This stress is a source of the side effects associated with its use. Ellagic acid (EA), a polyphenol is known to possess multiple health benefits owing to its antioxidant properties. EA is largely metabolized by the colon microbiota of different mammals and therefore was a polyphenol of choice in the present study. The present study was thus carried out to explore the protective potential of EA on CP induced hepatotoxicity in colon tumor bearing mice. The administration of EA (10 mg/kg bwt po daily for 6 weeks) significantly ameliorated the toxicity caused by CP (5 mg/kg bwt ip once a week for 4 weeks). Activities of liver marker enzymes and lactate dehydrogenase were brought back to normal. EA cotreatment also led to a marked reduction in the extent of peroxidative damage to liver tissue as was evident from the improvement in the histopathological changes observed and FT‐IR analysis. The present study, therefore, suggests that the administration of EA reduces the CP‐induced hepatotoxicity, thereby emerging out as a potential candidate for chemopreventive action.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Ellagic acid ameliorates cisplatin induced hepatotoxicity in colon carcinogenesis

Goyal

Koul

Ranawat

2019

Environmental Toxicology

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“…One of the main biomedical applications of FTIR micro-spectroscopy concerns the possibility to discriminate cancer cells from their normal counterpart according to specific spectral changes. Shen et al discriminated liver cancer cells from normal liver cells according to the intensity ratios of some specific FTIR peaks related to cellular components [10]. Li et al compared FTIR spectra of normal and several heterogeneous ovarian cancer cell lines and tissues, and they found spectral markers (consisting of relative FTIR band intensities) that were able to discriminate cancer samples from normal samples [11].…”

Section: Introductionmentioning

confidence: 99%

A Comparison between FTIR Spectra from HUKE and SH-SY5Y Cell Lines Grown on Different Substrates

2020

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In recent years, Fourier Transform Infrared (FTIR) micro-spectroscopy has shown promising potential in medical diagnostics at the cellular level. In fact, FTIR spectra can provide information related to DNA, protein, and lipid content and how such a content changes when a pathological state arises. Most of these information is included in the so-called fingerprint region (1000–1800 cm−1), consisting of several spectral peaks related to vibrational modes occurring inside cellular components. Unfortunately, the slides commonly used in cytology (as the glass microscopy slides and coverslips) are opaque to IR radiation in the fingerprint region, whereas they are transparent for wavenumber values larger than 2000 cm−1, where few and broad spectral absorption bands, mainly due to lipids and proteins, are present. Nonetheless, here we show that FTIR spectra performed in the high wavenumber range 2750–3000 cm−1 can be used to discriminate two different types of cells, one from a normal cell line (Human Keratinocyte, HUKE) and the other from a cancer one (SH-SY5Y). The spectra are discriminated by means of their Principal Component Analysis, according to the PC1 component, and by means of ratiometric analysis, according to the ratio of the intensity of the peak at 2956 cm−1 and that of the peak at 2924 cm−1. The PC1 score values of the HUKE are statistically different from the PC1 score values of SH-SY5Y, whereas the intensity ratio results larger for SH-SY5Y than for HUKE cells. Such results occur for different substrates over which the cells have been grown, including the thick glass slides used for cytological analysis. This result is a further step toward the application of FTIR microspectroscopy in the cytological routine diagnosis.

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“…In recent years, Fourier transform infrared (FTIR) spectroscopy has been widely used in the biochemical analysis field (Baker et al, 2014 ). FTIR spectroscopy is a simple, convenient, non-destructive, rapid and low-cost detection method to sample biological materials such as blood and tissue for diagnostic purposes (Deleris and Petibois, 2003 ; Ellis and Goodacre, 2006 ; Krafft et al, 2007 , 2009 ; Gasper et al, 2009 ; Gajjar et al, 2013 ; Baker et al, 2014 ; Mitchell et al, 2014 ; Ollesch et al, 2014 ; Sheng et al, 2015 ; Staniszewska-Slezak et al, 2015 ; Depciuch et al, 2017 ; Elmi et al, 2017 ; Ghimire et al, 2017 ; Guo et al, 2017 ; Le Corvec et al, 2017 ; Li et al, 2017 ; Liu et al, 2017 ; Paraskevaidi et al, 2017 ; Roy et al, 2017 ; Sarkar et al, 2017 ; Titus et al, 2017 ; De Bruyne et al, 2018 ; Rai et al, 2018 ). When combined with chemometric analysis, FTIR spectroscopy can be further empowered in disease diagnostics.…”

Section: Introductionmentioning

confidence: 99%

A Plasma Biochemical Analysis of Acute Lead Poisoning in a Rat Model by Chemometrics-Based Fourier Transform Infrared Spectroscopy: An Exploratory Study

et al. 2018

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In this work, we explored to use chemometrics-based Fourier transform infrared (FTIR) spectroscopy to investigate the plasma biochemical changes due to acute lead poisoning (ALP) in a rat model. We first collected the FTIR spectra of the plasma samples from the rats with and without suffering from ALP. We then performed the chemometric analysis of these FTIR spectra using principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA). We found that the chemometrics-based FTIR spectroscopy can discriminate the rats with and without ALP. Further analysis on the PLS-DA regression coefficient revealed that the spectral changes, in particular, corresponding to the biochemical changes of proteins in the plasma may be used as potential spectral biomarkers for the diagnostics of lead poisoning. Our work demonstrates the potential of chemometrics-based FTIR spectroscopy as a promising tool for the biochemical analysis of plasma that could consequently enable an objective, convenient and non-destructive diagnostics of lead poisoning. To the best of our knowledge, this work is the first application of chemometrics-based FTIR spectroscopy in the diagnostics of lead poisoning.

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A study of structural differences between liver cancer cells and normal liver cells using FTIR spectroscopy

Cited by 37 publications

References 30 publications

Ellagic acid ameliorates cisplatin induced hepatotoxicity in colon carcinogenesis

Ellagic acid ameliorates cisplatin induced hepatotoxicity in colon carcinogenesis

A Comparison between FTIR Spectra from HUKE and SH-SY5Y Cell Lines Grown on Different Substrates

A Plasma Biochemical Analysis of Acute Lead Poisoning in a Rat Model by Chemometrics-Based Fourier Transform Infrared Spectroscopy: An Exploratory Study

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