A study of the digestive enzyme activities in scaleless carp (Gymnocypris przewalskii) on the Qinghai-Tibetan Plateau

Tian, Haining; Meng, Yuqiong; Li, Changzhong; Zhang, Lunxiang; Guo-qian, XU; Shi, Yixiang; Shi, Jianquan; Qi, Hongfang; Ma, Rui

doi:10.1016/j.aqrep.2018.10.002

Cited by 5 publications

(10 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protease activity along the intestine did not differ between the anterior and posterior intestines, which indicated the presence of protein hydrolysis activity along the digestive tract of the Javaen barb. The results of this study were not different from previous studies on Glyptosternum maculatum, Lota lota, and Gymnocypris przewalskii, which showed similarities in protease activity between the anterior and posterior intestine (Xiong et al, 2011;Izvekova et al, 2013;Tian et al, 2019). In contrast, Sparindentex hasta, Labeo rohita, and Hoplias malabaricus showed decreased protease activity in the posterior intestine (Jahantigh, 2015; Umalatha et al, 2016;Gioda et al, 2017).…”

Section: Resultscontrasting

confidence: 70%

“…However, the protease activity shown in this study may differ from the activity of specific proteases such as trypsin and chymotrypsin. Previous studies have demonstrated in various species, such as Gymnocypris przewalskii, Mycrophis brachyurus, and Mystus nemurus which found the activity of trypsin and chymotrypsin in the intestine with optimal pH ranging from 9 to 10 ( Tian et al, 2019;Martinez-Cardenas et al, 2020;Rahmah et al, 2020). It appears that trypsin and chymotrypsin have optimal activity with a narrower pH range.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)

2022

View full text Add to dashboard Cite

Studies on morphology, growth, and reproduction have been carried out on wild Javaen barb, but there was no information on its digestive capacity; therefore, the research was conducted to determine protease and amylase activities in the digestive tract. This study used a total of 50 barbs with body weights between 13.56 -128.93g / fish. The measurement of enzyme activity was carried out using the spectrophotometer method. The results showed that differences in fish size resulted in differences in protease activity, but not for amylase. Fish with a small size have a higher protease activity than fish with a larger size. The protease activity did not differ between pH 6.9 to 10.0 but was higher than pH 12.5. Protease activity also did not vary between the anterior and posterior intestine and between 30-50°C. Amylase activity also found no difference between the anterior and posterior intestine, but there was a difference in activity between temperatures of 30-50°C. In conclusion, protease activity occurs in a neutral to alkaline environment, and there were differences in protease activity between different body sizes but not between intestinal segments. Amylase activity occurs throughout the intestine and decreases at temperatures of 50°C.

show abstract

Section: Resultscontrasting

confidence: 70%

Section: Resultsmentioning

confidence: 99%

Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)

2022

View full text Add to dashboard Cite

show abstract

“…The contribution of trypsin and chymotrypsin activities in fish is rather variable. For example, in scaleless carp (Gymnocypris przewalskii) the activity of trypsin is much higher than that of chymotrypsin (Tian et al, 2019), while in the Pacific bluefin tuna (Thunnus orientalis) chymotrypsin activity contributes more to digestion than trypsin activity (de la Parra et al, 2007). Even so, our observations in general agree with the notion that fish with carnivorous preferences show high proteolytic enzyme activities to digest high-protein animals, while herbivorous species exhibit higher carbohydrase activities to digest the storage carbohydrates of macroalgae (German et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili

2022

View full text Add to dashboard Cite

The study of fish digestive biochemistry is essential to understand factors that affect the net efficiency of food transformation and growth, and therefore aquaculture profitability. The aim of the present study was to assess the activity and functional characteristics of key digestive enzymes in juveniles of greater amberjack (Seriola dumerili), as well as the possible modulation of their relative importance by water temperature. For that, a combination of biochemical assays and substrate-SDS-PAGE were used. Under physiological conditions pepsin activity was negligible. Chymotrypsin was the most active enzyme in the digestive tract of the greater amberjack, while lipase was the enzyme with lower activity, though both enzymes in addition to trypsin were responsive to water temperature as revealed by discriminant analysis. Seriola dumerili showed to have pH-sensitive and, except for chymotrypsin, thermally robust proteases. Inhibition assays showed the major importance of serine proteases and revealed inverse trypsin and chymotrypsin responses to environmental temperature, with higher trypsin contribution in 26°C-fish while higher chymotrypsin contribution in 18°C-fish. Zymograms revealed three isotrypsin and three isochymotrypsin enzymes, with no variation in the presence of particular isoforms among rearing temperatures. However, they confirmed the role of chymotrypsin activity in providing digestive plasticity, with one of the isoforms being more active at lower temperatures. Thus, results indicate that variation in the relative contribution of chymotrypsin isoenzymes to a particular environmental temperature occurs due to different physic-chemical features of isoforms as a source of functional flexibility. This study assessed for the first time the effects of rearing temperature on greater amberjack digestive enzymes, increasing the knowledge on its digestive biochemistry, and aiding in the improvement of management practices for this species industrialization.

show abstract

“…This is an important step for optimal nutritional values and cost-effectiveness (Barlaya et al 2016). The utilization of nutrients in fish depends on the activities of digestive enzymes with different properties in the digestive organs (Tian et al 2019). The activity of digestive enzymes in fish may be changed depending on the fish age, feeding type, season, temperature (Munilla-Moran and Saborido-Rey, 1996), fish size (Murtaza et al 2016), feeding habits (Almeida et al 2018), time of day, infestation with parasites (Solovyev et al 2015) and intestinal morphology (Barlaya et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Monitoring of Enzymatic Activity in the Gastrointestinal Tract of Black Sea Salmon (Salmo labrax Pallas, 1814)

Özel¹,

Gürkan

2022

Acta Aquatica Turcica

View full text Add to dashboard Cite

Bu çalışmada, Karadeniz alabalığının (Salmo labrax) sindirim sistemi boyunca beslenme sonrası farklı zaman aralıklarında sindirim enzimlerinin aktivitesinin belirlenmesi amaçlanmıştır. Çalışma tatlısu ile çalışan kapalı devre araştırma ünitesinde gerçekleştirilmiştir. Balıklar deneme boyunca %46,25 protein ve %14,90 lipit içeren yemlerle 60 gün süresince doyana kadar elle beslenmiştir. Balıkların mide, ön (pilorik kese ile birlikte), orta ve arka bağırsakları içeren sindirim kanalı, yemlemeden sonra 45. dakika, 3., 6., 12., 24., 48., 72. ve 96. saatlerde alınmıştır. Dokular analiz edilene kadar -80°C'de saklanmıştır. Sindirim enzim aktivitesi beslenme sonrası 3. saatte ön ve orta bağırsak bölümlerinde en yüksek seviyeye ulaşmıştır. Benzer şekilde midedeki pepsin, tripsin ve lipaz enzimleri de beslenmeden sonraki 3. saatte en yüksek aktiviteye ulaşmıştır. Bununla birlikte, midede amilaz, beslenme sonrası 45. dakika ve 3. saatte en yüksek düzeyde bulunmuştur. Beslenme sonrası 3. saatte midede pepsin, ön bağırsakta tripsin ve lipaz ve orta kısımda amilaz en yüksek seviyelere ulaşmıştır. Bununla birlikte, bu enzimler arasındaki korelasyonlar pozitif yönde güçlü bulunmuştur. Ayrıca beslenme sonrası farklı zaman aralıklarında alınan örneklerde pepsin, tripsin, amilaz ve lipaz düzeylerindeki fark istatistiksel olarak anlamlı bulunmuştur (P<0.05). Sonuçlar, Karadeniz alabalığının sindirim kanalındaki mide, ön, orta ve arka bölümlerinin farklı seviyelerde enzim aktivitesine sahip olduğunu ve bu bölümlerin enzim aktivitesinin besleme sonrası zamana bağlı olarak değiştiğini göstermiştir.

show abstract

A study of the digestive enzyme activities in scaleless carp (Gymnocypris przewalskii) on the Qinghai-Tibetan Plateau

Cited by 5 publications

References 21 publications

Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)

Protease and Amylase Activities of Javaen barb (Systomus rubripinnis Val.)

Biochemical features and modulation of digestive enzymes by environmental temperature in the greater amberjack, Seriola dumerili

Monitoring of Enzymatic Activity in the Gastrointestinal Tract of Black Sea Salmon (Salmo labrax Pallas, 1814)

Contact Info

Product

Resources

About