A Study of the Lesions Induced in Seriola dumerili by Intradermal or Intraperitoneal Injection of Streptococcus dysgalactiae

Hagiwara, Hiroko; Takano, Ryoko; Noguchi, M.; Narita, Minoru

doi:10.1016/j.jcpa.2008.09.005

Cited by 18 publications

(20 citation statements)

References 17 publications

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“…Hagiwara et al . (, ) evaluated S. dysgalactiae infections in amberjack, based on experimental models. Those results indicated immersion or intradermal injection of amberjack, which would appear to be a more effective natural route than those involving oral or intraperitoneal exposure.…”

Section: Discussionmentioning

confidence: 99%

Genetic characteristics of Streptococcus dysgalactiae isolated from cage cultured cobia, Rachycentron canadum (L.)

Wang

Yoshida

et al. 2014

Journal of Fish Diseases

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Disease outbreaks occurred during 2007-2013 in Taiwan with 2.5-10% mortality among the cage cultured cobia, Rachycentron canadum (L.), characterized by the presence of polyserositis, pericarditis and peritonitis. The micro-organisms isolated from internal organs were Gram-positive cocci. The isolates were confirmed as Streptococcus dysgalactiae by a polymerase chain reaction assay that yielded the expected specific 259 bp amplicon. Additionally, partial sequence of the 16S-23S rDNA intergenic spacer region of the GCS strain isolates from fish was also compared and produced 100% sequence identity with S. dysgalactiae (GenBank accession number AB252398). The genetic characterization was then determined by pulsed-field gel electrophoresis (PFGE) analysis. Based on PFGE, the Apa I or Sma I digestion patterns of chromosomal DNA of these isolates were grouped into three main clusters. Taiwanese strains were divided into two clusters, and the tet(M) gene was detected in cluster 1 (pulsotypes: A1-A2 and S1-S3), but not in cluster 2 strains (pulsotypes: A3-A4 and S4-S5). Three Japanese strains from amberjack, Seriola dumerili (Risso), were grouped into cluster 3 (pulsotypes: A5-A7 and S6-S8) and displayed no mortality to cobia in the challenge experiment. Conversely, Taiwanese strains from cobia and snubnose pompano, Trachinotus blochii (L.), displayed a mortality rate of 50-87.5% in cobia.

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Section: Discussionmentioning

confidence: 99%

Genetic characteristics of Streptococcus dysgalactiae isolated from cage cultured cobia, Rachycentron canadum (L.)

Wang

Yoshida

et al. 2014

Journal of Fish Diseases

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“…dysgalactiae was reported to be associated with toxic shock-like syndrome in cattle (9), suppurative polyarthritis in lambs (28), bacteremia in dogs (66), and systemic granulomatous inflammatory disease and severe septicemia in fish (16) and in ascending upper limb cellulitis in humans in contact with raw fish (27). The presence of the streptococcal pyrogenic exotoxin G gene (spegg) and streptolysin S structural gene (sagA), which have been associated with invasive disease in the exclusively human pathogen Streptococcus pyogenes (group A Streptococcus [GAS]), has been documented for fish isolates of S. dysgalactiae subsp.…”

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confidence: 99%

Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

et al. 2011

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A custom-designed microarray containing 220 virulence genes of Streptococcus pyogenes (group A Streptococcus [GAS]) was used to test group C Streptococcus dysgalactiae subsp. dysgalactiae (GCS) field strains causing bovine mastitis and group C or group G Streptococcus dysgalactiae subsp. equisimilis (GCS/GGS) isolates from human infections, with the latter being used for comparative purposes, for the presence of virulence genes. All bovine and all human isolates carried a fraction of the 220 genes (23% and 39%, respectively). The virulence genes encoding streptolysin S, glyceraldehyde-3-phosphate dehydrogenase, the plasminogen-binding M-like protein PAM, and the collagen-like protein SclB were detected in the majority of both bovine and human isolates (94 to 100%). Virulence factors, usually carried by human beta-hemolytic streptococcal pathogens, such as streptokinase, laminin-binding protein, and the C5a peptidase precursor, were detected in all human isolates but not in bovine isolates. Additionally, GAS bacteriophage-associated virulence genes encoding superantigens, DNase, and/or streptodornase were detected in bovine isolates (72%) but not in the human isolates. Determinants located in non-bacteriophage-related mobile elements, such as the gene encoding R28, were detected in all bovine and human isolates. Several virulence genes, including genes of bacteriophage origin, were shown to be expressed by reverse transcriptase PCR (RT-PCR). Phylogenetic analysis of superantigen gene sequences revealed a high level (>98%) of identity among genes of bovine GCS, of the horse pathogen Streptococcus equi subsp. equi, and of the human pathogen GAS. Our findings indicate that alphahemolytic bovine GCS, an important mastitis pathogen and considered to be a nonhuman pathogen, carries important virulence factors responsible for virulence and pathogenesis in humans.

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“…Epicarditis and severe necrotic legions of the caudal peduncle are typical symptoms of S. dysgalactiae infection in the farmed genus Seriola . However, its virulence mechanisms are poorly understood and there is little information on the virulence factors present in S. dysgalactiae fish isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Since 2002, Lancefield group C S. dysgalactiae has been isolated from farmed fish, such as great amberjack ( Seriola dumerili ), yellowtail ( S. quinqueradiata ), and kingfish ( S. lalandi ), in Japan . S. dysgalactiae infection in fish is characterized by pericarditis and severe necrotic lesions in the caudal peduncle . S. dysgalactiae isolated from fish are regarded as S. dysgalactiae subsp.…”

Section: Introductionmentioning

confidence: 99%

Complete genome sequence and characterization of virulence genes in Lancefield group C Streptococcus dysgalactiae isolated from farmed amberjack (Seriola dumerili)

Nishiki

Yoshida

Fujiwara

2019

Microbiology and Immunology

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Lancefield group C Streptococcus dysgalactiae causes infections in farmed fish. Here, the genome of S. dysgalactiae strain kdys0611, isolated from farmed amberjack (Seriola dumerili) was sequenced. The complete genome sequence of kdys0611 consists of a single chromosome and five plasmids. The chromosome is 2,142,780 bp long and has a GC content of 40%. It possesses 2061 coding sequences and 67 tRNA and 6 rRNA operons. One clustered regularly interspaced short palindromic repeat, 125 insertion sequences, and four predicted prophage elements were identified. Phylogenetic analysis based on 126 core genes suggested that the kdys0611 strain is more closely related to S. dysgalactiae subsp. dysgalactiae than to S. dysgalactiae subsp. equisimilis. The genome of kdys0611 harbors 87 genes with sequence similarity to putative virulence‐associated genes identified in other bacteria, of which 57 exhibit amino acid identity (>52%) to genes of the S. dysgalactiae subsp. equisimilis GGS124 human clinical isolate. Four putative virulence genes, emm5 (FGCSD_0256), spg_2 (FGCSD_1961), skc (FGCSD_1012), and cna (FGCSD_0159), in kdys0611 did not show significant homology with any deposited S. dysgalactiae genes. The chromosomal sequence of kdys0611 has been deposited in GenBank under Accession No. AP018726. This is the first report of the complete genome sequence of S. dysgalactiae isolated from fish.

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A Study of the Lesions Induced in Seriola dumerili by Intradermal or Intraperitoneal Injection of Streptococcus dysgalactiae

Cited by 18 publications

References 17 publications

Genetic characteristics of Streptococcus dysgalactiae isolated from cage cultured cobia, Rachycentron canadum (L.)

Genetic characteristics of Streptococcus dysgalactiae isolated from cage cultured cobia, Rachycentron canadum (L.)

Virulence Gene Pool Detected in Bovine Group C Streptococcus dysgalactiae subsp. dysgalactiae Isolates by Use of a Group A S. pyogenes Virulence Microarray

Complete genome sequence and characterization of virulence genes in Lancefield group C Streptococcus dysgalactiae isolated from farmed amberjack (Seriola dumerili)

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