A study of the mobility of main and side chains of macromolecules by polarized luminescence

SynopsisPolarized luminescence was used to study the mobility of tryptophan residues in polypeptide chains of different chemical composition and structural organization. It has been shown that the luminescence depolarization of tryptophan residues in coillike, helical, and p-structural polypeptide chains is mainly caused by "fast" torsional vibrations and "slow" rotational isomerization of indole groups of tryptophan side chains. The characteristics of these types of motions are practically the same for tryptophan residues included in coillike chains of different chemical structure. Helix-coil transitions in copolymers of glutamic acid and lysine with tryptophan (Glu, Trp) and (Lys, Trp) (where side groups of tryptophan residues weakly interact with the surrounding side groups) do not appreciably change the amplitude of torsional vibrations or rotational isomerization. At the same time, in the helical state of glutamic acid-leucine-tryptophan copolymers (Glu, Leu, Trp) and in the p-structural state of (Lys, Trp) copolymers (where direct interactions of Trp side groups with other side groups are possible), the amplitudes of the torsional vibrations are smaller and the rotational isomerization times larger than in the coil. The transition of (Glu, Leu, Trp) polypeptide chains into a compact state is accompanied by a marked decrease of both "fast" and "slow" intramolecular mobility and by an increase of the contribution made by the rotation of the macromolecule as a whole, as shown by the decrease of the luminescence polarization.

show abstract

“…4 Then Eq. where f is the total contribution of "fast" motions to the decrease in luminescence polarization.…”

Section: Theorymentioning

confidence: 99%

“…Using Eqs. (3) and (4) it is possible to calculate from these dependences the average time of "slow" relaxation processes r and the relative contribution of "fast" relaxation processes to the decrease in luminescence polarization f. [The correction distinguishing Eq. (4') from Eq.…”

Section: Separation Of "Fast" and "Slow" Relaxation Processes For Coimentioning

confidence: 99%

Polarized luminescence and mobility of tryptophan residues in polypeptide chains

et al. 1981

View full text Add to dashboard Cite

show abstract

The kinetics of the photochemical reduction of anthracene groups attached to macromolecules in the presence of p-toluidine

Kozel¹,

Lashkov²,

Gotlib³

et al. 1978

Polymer Science U.S.S.R.

View full text Add to dashboard Cite

Hydrodynamic interactions and their effect on local high frequency processes in the polymer chain (Polarized luminescence, NMR)

Gotlib¹,

Svetlov²,

Torchinskii³

1979

Polymer Science U.S.S.R.

View full text Add to dashboard Cite

A study of the mobility of main and side chains of macromolecules by polarized luminescence

Cited by 6 publications

References 4 publications

Polarized luminescence and mobility of tryptophan residues in polypeptide chains

Polarized luminescence and mobility of tryptophan residues in polypeptide chains

The kinetics of the photochemical reduction of anthracene groups attached to macromolecules in the presence of p-toluidine

Hydrodynamic interactions and their effect on local high frequency processes in the polymer chain (Polarized luminescence, NMR)

Contact Info

Product

Resources

About