A Study of the Properties of the Rhesus
Antibody (Ri) Diagnostic for the Rheumatoid Factor
and its Application to Gm Grouping

Waller, Marion; Lawler, Sylvia D.

doi:10.1159/000464824

Cited by 13 publications

(16 citation statements)

References 13 publications

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“…Two human sera with strong anti-D activity (indirect Coombs titers 1: 256 and 1: 1024) were obtained from the blood bank (Blood Services of New Mexico). The hyperimmune anti-CD serum Ripley (12) Papain digestion was performed according to the method of Porter (18). Digestion was carried out for 24 hr using a 1: 100 enzyme-to-protein ratio.…”

Section: Methodsmentioning

confidence: 99%

Receptors for human γG Globulin on human neutrophils

Messner¹,

Jelinek²

1970

J. Clin. Invest.

219

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Section: Methodsmentioning

confidence: 99%

Receptors for human γG Globulin on human neutrophils

Messner¹,

Jelinek²

1970

J. Clin. Invest.

219

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“…A number of sera were screened simultaneously with two incomplete anti-D coats of comparable titer, one (2368) agglutinated infrequently by rheumatoid sera and the other (Rip.) recognized for its broad reactivity with rheumatoid factor (28). Both are of use as Gm(a) and (x) typing reagents when used with appropriate agglutinators.…”

Section: Methodsmentioning

confidence: 99%

Antibodies against gamma-globulin after repeated blood transfusions in man.

Allen¹,

Kunkel²

1966

J. Clin. Invest.

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“…These human sera were largely derived from five major sources: (a) grand multiparous women previously sensitized by multiple pregnancies (19 sera); (b) 8 sera obtained from patients who had received multiple transfusions; (c) 5 sera derived from patients with disseminated lupus erythematosus or diffuse vaculitis; (d) 15 sera from patients with cold agglutinins associated with viral pneumonia or chronic cold agglutinin disease; and (e) 4 sera from patients with subacute bacterial endocarditis.…”

Section: Methodsmentioning

confidence: 99%

“…The possibility still remained that the high molecular weight aG cytotoxic complexes consisted of complexes of 7S anti-HL-A specific antibody plus molecules of 7S yG rheumatoid factor. Accordingly, all cytotoxic sera or isolated fractions were tested for anti-human y-globulin activity using Rhpositive CD human cells coated with incomplete anti-CD antibody Ripley (15). Although many of the sera from the multiply transfused patients were positive for antihuman Y-globulin antibody by this technique, many strongly cytotoxic sera showed no detectable anti-yglobulin antibody.…”

Section: Methodsmentioning

confidence: 99%

Studies of human sera with cytotoxic activity

Williams¹,

Emmons²,

Yunis³

1971

J. Clin. Invest.

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A B S T R A C T 42 human sera showing in vitro cytotoxic activity of restricted or broad HL-A specificities with test human lymphocytes were studied for the molecular and immunoglobulin class of cytotoxic antibody using sucrose gradient separations, DEAE-cellulose chromatography, and Sephadex G-200 gel filtration. Sera originated from patients with previous multiple pregnancies (19), multiply transfused patients (8), subacute bacterial endocarditis (4), systemic lupus (2), and human umbilical cord sera (9). In 32 of 42 instances, predominant cytotoxic activity was found in high molecular weight gradient fractions; however, DEAE chromatographic separations revealed cytotoxic activity in initial buffer fractions containing primarily -vG globulin. Gradient separations of cytotoxic activity within initial 'vG DEAE fractions showed localization of cytotoxicity only in high molecular weight materials.Confirmation of high molecular weight YG cytotoxic activity was obtained by resistance to mercaptoethanol treatment, abolition of activity after absorption only with specific anti-yG antisera, and by the finding that high molecular weight cytotoxic activity in gradients or gel filtration run at neutral pH 7.4 became 7S when separations were rerun at an acidic pH of 4.0. Such 7S activity again became rapidly sedimenting when the same fractions were again rerun in gradients at neutral pH.19S iM cytotoxic activity was documented in a panel of 15 human sera containing anti-"I" cold agglutinins. In this instance the cytotoxic activity appeared to be related to the cold agglutinin antibody since it was mercaptoethanol sensitive and could be demonstrated in monoclonal antibody eluates containing primarily -yM.

show abstract

A Study of the Properties of the Rhesus Antibody (Ri) Diagnostic for the Rheumatoid Factor and its Application to Gm Grouping

Cited by 13 publications

References 13 publications

Receptors for human γG Globulin on human neutrophils

Receptors for human γG Globulin on human neutrophils

Antibodies against gamma-globulin after repeated blood transfusions in man.

Studies of human sera with cytotoxic activity

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