A study of the relationships of changes in pain and gait after tourniquet-induced ischemia-reperfusion in rats

Abstract

[Purpose] The purpose of this study was to determine the relationships of changes in pain and gait after ischemia reperfusion was induced by tourniquet in rats. [Subjects and Methods] The subjects were six ten-week-old male Wistar rats. Ischemia was induced in the left lower limbs of the experimental rats at a pressure of 300 mmHg for 90 minutes. Pain behavior evaluations were measured using the von Frey test in all the rats’ hind limbs. A consistently increasing plantar stimulus was applied until the rats exhibited an escape behavior. For the evaluation of gait, a two-dimensional motion analysis system was used to measure the distance from the calcaneus to the floor (DCF) and toe extension angle (TEA) during gait. The evaluations were performed in the normal state, 3 hours after ischemia-reperfusion, and daily until 7 days after ischemia-reperfusion. [Results] Compared with the normal state, the means of the pain threshold showed a significant decrease until 4 days after ischemia. In addition, both TEA and DCF continued to show a significant decrease at 7 days after ischemia as compared with the normal state. [Conclusion] This study revealed that hyperalgesia occurs after ischemia-reperfusion, and recovery of hyperalgesia occurred earlier than gait dysfunction recovery.

Mitochondrial transplantation ameliorates acute limb ischemia

“…Percentage shared stance time, defined as the percentage of the stance time that both hind paws are in contact with the treadmill belt at the same time, was significantly increased, mimicking increased time in double supports seen in patients with limb ischemia. [41][42][43][44][45] We also show that stance factor, related to decreased power in the hindlimb, was significantly decreased after ischemia, as evidenced by dragging of the hindlimb. This evaluation has been primarily used in crushed nerve injuries to show decreased peripheral limb function due to nerve damage.…”

Extended Tourniquet Times and the Impact on Wound Healing in Foot Surgery

“…Both cohorts did not demonstrate any incidence of compartment syndrome, major blood loss or DVT, comparable to current literature. 3,11,17 One weakness of this trial is the lack of a formalized wound assessment tool used in clinic and thus we have some operator dependent variability. Infection was regarded as stated in clinic or a prescription of antibiotics by the clinician or general practitioner after wound review in this timeframe, which potentially excludes some patients with milder infections or those who received antibiotics in a different health board.…”

Morphological illustration of alterations in the arterial endothelium in ischemic and reperfusion injuries

“…Эти патологические процес-сы являются следствием не только пред-шествовавшей ишемии конечности, но и последующей реперфузии [6]. Многогран-ность патофизиологических процессов оп-ределяет важность проведения экспери-ментальных работ в изучении данного фе-номена [7] с возможностью сопоставления и сравнения глубины морфологических изменений эндотелия на различных этапах хирургической коррекции.…”