A Study on Antitumor Effect of 1,3,4-Thiadiazole Derivatives in Prostate and Breast Cancer Cell Lines (<em>In Vitro</em>)

Abstract

Abstract5-(4-aminophenyl )-2-amino -1,3,4-thiadiazole was prepared by reaction of Thiosemicarbazide with 4-amino benzoic acid under reflux condition for 7 hours. The compound which has been synthesized successfully was subjected to addition reaction with 4-(Dimethylamino) benzaldehyde under reflux condition for 6 hours to synthesize Schiff bases. These compounds was characterized by using FTIR) and evaluated for their anticancer activity. The effect of (1, 3, 4-thiadiazole derivative) on the activity of malignant cells was studied by using different types of cell lines [Breast cancer, and human prostate cancer]. And was used the Electron microscope to show that the effect of the derivative on the cancer cells before and after 3 days of the injection time. It was found that the Schiff base of thiadiazole-1,3,4-(Dimethylamino)benzylidineamino]-[4-2-phenyl]amino was effective in reducing the size and density of malignant cells. That of 46.7 while in breast ) 145(DUprostate for growth inhibition produce of equal 85.9 µg/ml.

Cytotoxicity evaluation of CELNORM, a nutritional health supplement, on MCF7 breast cancer cells

“…Heterocyclic compounds such as 1,3,4-thiadiazole derivatives have been shown to cause cytotoxicity in breast and prostate cancer cells in vitro. Eupatorine, a methoxy flavone flavonoid was reported to induce apoptosis and concurrently inhibit the invasion, migration and angiogenesis of MDA-MB-231 and MCF-7 cells through inhibition of Phospho-Akt pathway and cell cycle blockade (7)(8)(9)(10) . It is a well-known fact that the treatment of cancers involves the use of chemotherapeutic drugs and /or radiation depending on a variety of factors.…”

Novel Para-Aminobenzoic Acid Analogs and Their Potential Therapeutic Applications

“…Azoles and azines were formed by the reaction of PABA with isothiocyanate and heteroallene [96]. A thiadiazole-based compound was synthesized by the reaction of PABA with thiosemicarbazide upon refluxing for up to 7 h. Schiff bases were obtained through the reaction with dimethylaminobenzaldehyde at a reflux temperature for 6 h [97]. A series of quinazolinone derivatives were obtained: 3-(4-oxo-2-phenylquinazolin-3(4H)-yl)benzoic acid and 4-(4-oxo-2-phenyl quinazolin-3(4H)-yl)benzoic acid.…”

“…A study of the activity of the malignant cell activity of a synthetic 1,3,4-thiadiazole derivative observed the effectiveness of the Schiff base of thiadiazole-1,3,4-(dimethylamino)benzylidineamino]-[4-2-phenyl]amino in decreasing the density and size and of malignant cells [97]. One of the synthetic compounds, hexadecyl 4-(4-oxo-2-phenylquinazolin-3(4H)-yl)benzoate, displayed notable cytotoxic activity with IC 50 values of 23.31 ± 0.09 µM, 72.22 ± 0.14 µM, and 53.29 ± 0.25 µM against Caco-2, MCF-7, and HepG2 cancer cells, respectively [98].…”

A Review on Synthesis, Therapeutic, and Computational Studies of Substituted 1, 3, 4 Thiadiazole Derivatives

“…Among them, N-benzyl- 145) DUprostate for growth inhibition produce of equal 85.9 μg/ml. 54 A novel series of bis(1,3,4-thiadiazole) derivatives 76 (Scheme 45) were synthesized in one step methodology with good yields by condensation reaction between bis-hydrazonoyl chloride and various reagents. The structures of the prepared compounds were confirmed by spectral data (IR, NMR, and MS), and elemental analysis.…”