A Study on Relationship of Plasmid with Antibiotic Resistance in Thermophilic Campylobacter spp. Isolates from Environmental Samples

Baserisalehi, Majid; Bahador, Nima

doi:10.3923/biotech.2008.813.817

Cited by 10 publications

(15 citation statements)

References 18 publications

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“…Plasmids were detected in 4.5% of C. jejuni strains isolated from sheep and 27% of C. coli strains isolated from rhesus monkey, swine and poultry [ 19 ]. Environmental strains of C. jejuni and C. coli were found to contain plasmids at percentages of 60% and 50% respectively [ 20 ]. Thirty-two percent of Campylobacter isolates from the Seattle County Department of Public health were found to harbor plasmids [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats

Marasini

Fakhr

2014

Pathogens

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Campylobacter spp. is one of the most prevalent bacterial pathogens in retail meat, particularly poultry, and is a leading cause of diarrhea in humans. Studies related to Campylobacter large plasmids are limited in the literature possibly due to difficulty in isolating them using available alkaline lysis methods. The objectives of this study were to determine the prevalence of plasmids, particularly large ones, in Campylobacter spp. isolated from various Oklahoma retail meats, and to explore PFGE (Pulsed Field Gel Electrophoresis) as a tool in facilitating the detection of these plasmids. One hundred and eighty nine strains (94 Campylobacter jejuni and 95 Campylobacter coli) were screened for the presence of plasmids using both alkaline lysis and PFGE. Plasmids were detected in 119/189 (63%) using both methods. Most of the plasmids detected by alkaline lysis were smaller than 90 kb and only three were larger than 90 kb. Plasmids over 70 kb in size were detected in 33 more strains by PFGE of which 11 strains contained larger than 90 kb plasmids. Plasmids were more prevalent in Campylobacter coli (73.5%) than in Campylobacter jejuni (52%). BglII restriction analysis of plasmids isolated from 102 isolates revealed 42 different restriction patterns. In conclusion, PFGE was able to detect large plasmids up to 180 Kb in Campylobacter spp. which might have been missed if the alkaline lysis method was solely used. Campylobacter spp. isolated from retail meats harbor a diverse population of plasmids with variable sizes. To our knowledge, this is the first study to use PFGE to detect large plasmids in Campylobacter.

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Section: Introductionmentioning

confidence: 99%

Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats

Marasini

Fakhr

2014

Pathogens

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“…In order to test the transformational efficiency of the transposon mutant of cj1051c, several attempts were made to cure it of pWM1007 with marker-free subculturing, heat and oxidative stress, and rifampin treatment (4). As this method failed, a Tet r knockout strain of cj1051c (JH1) as well as a complementation strain (JH2) were produced.…”

Section: Mutant Screen For Transformationmentioning

confidence: 99%

Identification of Cj1051c as a Major Determinant for the Restriction Barrier of Campylobacter jejuni Strain NCTC11168

Holt

Grant

Coward

et al. 2012

Appl Environ Microbiol

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cCampylobacter jejuni is a leading cause of human diarrheal illness in the world, and research on it has benefitted greatly by the completion of several genome sequences and the development of molecular biology tools. However, many hurdles remain for a full understanding of this unique bacterial pathogen. One of the most commonly used strains for genetic work with C. jejuni is NCTC11168. While this strain is readily transformable with DNA for genomic recombination, transformation with plasmids is problematic. In this study, we have identified a determinant of this to be cj1051c, predicted to encode a restriction-modification type IIG enzyme. Knockout mutagenesis of this gene resulted in a strain with a 1,000-fold-enhanced transformation efficiency with a plasmid purified from a C. jejuni host. Additionally, this mutation conferred the ability to be transformed by plasmids isolated from an Escherichia coli host. Sequence analysis suggested a high level of variability of the specificity domain between strains and that this gene may be subject to phase variation. We provide evidence that cj1051c is active in NCTC11168 and behaves as expected for a type IIG enzyme. The identification of this determinant provides a greater understanding of the molecular biology of C. jejuni as well as a tool for plasmid work with strain NCTC11168.

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“…In 2003, 95% of antibiotic resistant P. aeruginosa isolated from burn patients in Tehran hospitals harbored plasmid ( 24 ). In addition, plasmid curing confirmed the relationship between antibiotics resistance character and the existence of plasmid ( 25 ). In the present study cured A. baumanii and P. aeruginosa lost their resistance markers after losing their plasmids.…”

Section: Discussionmentioning

confidence: 73%

Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients

Beige

Salehi

Bahador

et al. 2014

Jundishapur J Microbiol

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Background:Nowadays, the treatment of burned patients is difficult because of the high frequency of infection with antibiotic resistance bacteria.Objectives:This study was conducted to evaluate the level of antibiotic resistance in Gram-negative bacteria and its relation with the existence of plasmid.Materials and Methods:The samples were collected from two hundred twenty hospitalized burned patients in Isfahan burn hospital during a three-month period (March 2012 to June 2012). The samples were isolated and the Gram-negative bacteria were identified using phenotypic method and API 20E System. Antibiotic susceptibility and plasmid profile were determined by standard Agar disc diffusion and plasmid spin column extraction methods.Results:Totally 117 Gram-negative bacteria were isolated, the most common were Pseudomonas aerugionsa (37.6%), P. fluorescens (25.6%), Acinetobacter baumanii (20/5%) and Klebsiella pneumoniae (7.6%), respectively. The isolates showed high frequency of antibiotic resistance against ceftazidime and co-amoxiclave (100%) and low frequency of antibiotic resistance against amikacin with (70%).The results indicated that 60% of the isolates harboured plasmid. On the other hand, the patients infected with A. baumanii and P. aeruginosa were cured (with 60% frequency) whereas, those infected with P. fluorescens were not cured. Hence, probably antibiotic resistance markers of A. baumanii and P. aeruginosa are plasmid mediated; however, P. fluorescens is chromosomally mediated.Conclusions:Based on our findings, P. aerugionsa is a major causative agent of wound infections and amikacin could be considered as a more effective antibiotic for treatment of the burned patients.

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A Study on Relationship of Plasmid with Antibiotic Resistance in Thermophilic Campylobacter spp. Isolates from Environmental Samples

Cited by 10 publications

References 18 publications

Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats

Exploring PFGE for Detecting Large Plasmids in Campylobacter jejuni and Campylobacter coli Isolated from Various Retail Meats

Identification of Cj1051c as a Major Determinant for the Restriction Barrier of Campylobacter jejuni Strain NCTC11168

Plasmid Mediated Antibiotic Resistance in Isolated Bacteria From Burned Patients

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