A Study on the Effective Ratio of Fat to Stromal Vascular Fraction for Cell-Assisted Lipotransfer

Bae, Yong Chan; Kim, Kyoung Hoon; Yun, Hye Jin; Oh, Chang Hyun; Chang, Jae Hoon; Yi, Chang; Lee, Jae Woo; Bae, Seong Hwan

doi:10.1007/s00266-019-01548-6

Cited by 13 publications

(5 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While supplemented ASC numbers are very well known as the major regulatory factor in determining the high survival rate of fat grafts [ 25 ], the optimal concentration of ASCs in fat grafts has not been established [ 36 , 37 , 38 ], mainly due to the wide range of ASC cell quality in cell-assisted transfer. Moreover, in clinical applications, ASC numbers from donor fat tissue are limited; thus, additional modifications, such as excess fat tissue harvesting and in vitro ASC culture, are needed [ 39 , 40 ]. The development of a method to increase ASC potential could solve the problem of the current CAL procedure.…”

Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

View full text Add to dashboard Cite

Cell-assisted lipotransfer (CAL), defined as co-transplantation of aspirated fat with enrichment of adipose-derived stem cells (ASCs), is a novel technique for cosmetic and reconstructive surgery to overcome the low survival rate of traditional fat grafting. However, clinically approved techniques for increasing the potency of ASCs in CAL have not been developed yet. As a more clinically applicable method, we used mechanical stress to reinforce the potency of ASCs. Mechanical stress was applied to the inguinal fat pad by needling . Morphological and cellular changes in adipose tissues were examined by flow cytometric analysis 1, 3, 5, and 7 days after the procedure. The proliferation and adipogenesis potencies of ASCs were evaluated. CAL with ASCs treated with mechanical stress or sham control were performed, and engraftment was determined at 4 weeks post-operation. Flow cytometry analysis revealed that mechanical stress significantly increased the number as well as the frequency of ASC proliferation in fat. Proliferation assays and adipocyte-specific marker gene analysis revealed that mechanical stress promoted proliferation potential but did not affect the differentiation capacity of ASCs. Moreover, CAL with cells derived from mechanical stress-treated fat increased the engraftment. Our results indicate that mechanical stress may be a simple method for improving the efficacy of CAL by enhancing the proliferation potency of ASCs.

show abstract

Section: Resultsmentioning

confidence: 99%

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Chun

Chang

Soedono

et al. 2022

IJMS

View full text Add to dashboard Cite

show abstract

“…44 Other studies confirmed the aforementioned results and showed that a cSVF/lipofilling ratio higher than 1:1 did not increase the survival rate in animal models. 45,46 A higher survival rate of transplanted SVF-enriched fat grafts might also result in a greater regenerative effect on the overlying skin. Compared with our study, these animal models used a significantly higher ratio of cSVF/lipofilling and therefore patients might be undertreated in this study.…”

Section: Discussionmentioning

confidence: 99%

The Addition of Tissue Stromal Vascular Fraction to Platelet-Rich Plasma Supplemented Lipofilling Does Not Improve Facial Skin Quality: A Prospective Randomized Clinical Trial

Dongen

Boxtel

Willemsen

et al. 2021

Aesthetic Surgery Journal

View full text Add to dashboard Cite

Background Lipofilling has become popular as a treatment to improve aging related skin characteristics (eg, wrinkles, pigmentation spots, pores, or rosacea). Different additives such as platelet-rich plasma (PRP) or stromal vascular fraction (SVF) have been added to lipofilling to increase the therapeutic effect of adipose derived stromal cells (ASCs). Objectives In this study, we hypothesized that mechanical isolated SVF augments the therapeutic effect of PRP supplemented lipofilling to improve facial skin quality. Methods This prospective, double-blinded, placebo-controlled, randomized trial was conducted between 2016 and 2019. In total, 28 female subjects were enrolled with 25 completing the follow-up. All patients received PRP supplemented lipofilling with either mechanical isolated SVF or saline. SVF was isolated by means of the fractionation of adipose tissue (FAT) procedure (tissue-SVF). Results were evaluated by changes in skin elasticity and transepidermal water loss, changes in skin aging related features, ie, superficial spots, wrinkles, skin texture, pores, vascularity, and pigmentation as well as patient satisfaction (FACE-Q), recovery, and number of complications up to 1 year postoperative. Results The addition of tSVF to PRP supplemented lipofilling did not improve skin elasticity, transepidermal water loss nor skin aging related features. No improvement in patient satisfaction with overall facial appearance nor facial skin quality was seen when tSVF was added to PRP supplemented lipofilling. Conclusions PRP supplemented lipofilling with tSVF compared to PRP supplemented lipofilling alone does not improve facial skin quality nor patient satisfaction in a healthy population. PRP supplemented lipofilling with tSVF can be considered a safe procedure.

show abstract

“…Mild and moderate defects are treated mainly with biomaterials and autologous adipose tissue. Adipose tissue grafting is a common procedure for soft tissue reconstruction, but with a 20-80% probability of reabsorption (to prevent its effects to some extent, the so-called volume overcorrection at the augmentation site is used) [19,36,[45][46][47]. Its pioneers were Illouz, Fournier and Otteni.…”

Section: Treatmentmentioning

confidence: 99%

Disturbances of the stomatognathic system and possibilities of its correction in patients with craniofacial morphea

Wróblewska,

Polańska,

Mojs

et al. 2023

pdia

View full text Add to dashboard Cite

Morphea en coup de sabre and progressive hemifacial atrophy are extremely rare connective tissue disorders causing facial deformity. In extreme cases, morphological disorders are accompanied by symptoms of a clear impairment of the stomatognathic system. The aetiology of the above-mentioned diseases is still unknown. Properly planned therapy in the field of maxillofacial orthopaedics makes it possible to correct the asymmetric pattern of hard tissue growth and thus enable rehabilitation. The task of augmentation techniques is the volumetric supplementation of tissue defects resulting from atrophic processes. The degree of destruction and the extent of changes determine the method of correction. Mild and moderate defects are treated mainly with biomaterials and autologous adipose tissue. The severe course of hemifacial atrophy and morphea en coup de sabre and the associated significant tissue atrophy necessitate the search for more complex methods of treatment. In this paper, we summarize the disturbances of the stomatognathic system in patients with craniofacial morphea, together with an analysis of current treatment options.

show abstract

A Study on the Effective Ratio of Fat to Stromal Vascular Fraction for Cell-Assisted Lipotransfer

Cited by 13 publications

References 12 publications

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

Mechanical Stress Improves Fat Graft Survival by Promoting Adipose-Derived Stem Cells Proliferation

The Addition of Tissue Stromal Vascular Fraction to Platelet-Rich Plasma Supplemented Lipofilling Does Not Improve Facial Skin Quality: A Prospective Randomized Clinical Trial

Disturbances of the stomatognathic system and possibilities of its correction in patients with craniofacial morphea

Contact Info

Product

Resources

About