A Study on the Mechanism of Intercellular Adhesion

Deman, Joris; Bruyneel, Erik; Mareel, Marc

doi:10.1083/jcb.60.3.641

Cited by 70 publications

(15 citation statements)

References 52 publications

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“…for dissociating brain and other tissues [16]. Conversely, studies on HeLa cells have shown that calcium ions promote aggregation of the cells [6]. Moreover, it has been pro posed that DPH may interact competitively with calcium at the neuronal membrane [19,25], Thus additional studies to investigate the possibility that an alteration in calcium dynamics plays a role in the morphological effects observed with DPH treatment of brain cell cultures seem to be warranted.…”

Section: Discussionmentioning

confidence: 99%

Effects of Anticonvulsant Drugs on Chick Embryonic Neurons and Glia in Cell Culture

Culver¹,

Vernadakis²

1979

Dev Neurosci

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The responses of neurons and glial cells to diphenylhydantoin (DPH), 5-(p-hydroxyphenyl)-5-phenylhydantoin (HPPH) and phenobarbital were studied in cell cultures of dissociated chick embryo brain. Drugs were added to cultures daily for 3 days in concentrations ranging from 1 x 10^–4 to 1 x 10^–3M. Histological evaluations revealed that cultures treated with DPH exhibited fewer cell aggregates, fewer neurons and less prominent neuronal processes. Preliminary studies suggested that the degree of neurotoxicity might vary in different brain regions and at different stages of neural development. Cultures treated with HPPH exhibited toxicity similar to those treated with DPH but phenobarbital-treated cultures were not significantly different than control cultures. Incorporation of [¹⁴C]-leucine into protein was also decreased in cultures treated with DPH but not in those treated with phenobarbital.

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Section: Discussionmentioning

confidence: 99%

Effects of Anticonvulsant Drugs on Chick Embryonic Neurons and Glia in Cell Culture

Culver¹,

Vernadakis²

1979

Dev Neurosci

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“…The possibility that Ca2+ acts as an intercellular bridge (24) or that this ion is a necessary component for the function of intercellular macromolecules (8,25,27) to bind apposed cell surfaces should be taken into consideration in speculations on the nature of the "Ca-mechanism." 28.…”

Section: Discussionmentioning

confidence: 99%

Two Mechanisms in Cell Adhesion Revealed by Effects of Divalent Cations

Ueda

Takeichi

1976

Cell Struct. Funct.

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ABSTRACT. The effect of divalent cations on cell-to-cell and cell-to-substrate adhesions was studied using two different types of chicken embryonic cells (fibroblastic and neural retina cells). Mn2+, Co2+, Mg2+ and Ca2+ were effective in promoting the aggregation of fibroblastic cells, while Sr2+ and Ba2+ were not effective. Among these ions, Ca2+ showed some unique effects on cell aggregation. Aggregation was initiated immediately after the addition of Mn2+, Co2+ or Mg2+ whereas with Ca2+ a lag period was always observed. When the medium contained both Ca2+ and Mg2+, the aggregate size formed was bigger than that formed with either ion at an equimolar concentration. The divalent cation dependence of fibroblastic cell adhesion to non-cellular substrate was essentially the same as that of cell-to-cell adhesion. The aggregation of neural retina cells specifically depended on the presence of Ca2+. For adhesion of neural retina cells to non-cellular substrate, however, Ca2+ was not effective, and only Mn2+ or Co2+ was effective. These results suggest two different mechanisms in cell adhesion.

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“…In rat cultures, the structural architecture of the spheroids and expression of CYP 450 enzymes and HGF are also determined. Monolayer monocultures and aggregate monocultures formed by agitated culture or trypsin pre-treatment [Deman et al, 1974] are used as controls.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Three-Dimensional Co-Culture of Hepatocytes and Hepatic Stellate Cells on Key Hepatocyte Functions in vitro

Thomas

Bhandari

Barrett

et al. 2005

Cells Tissues Organs

115

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In this study, we demonstrate the ability of a three-dimensional co-culture model to preserve some key aspects of differentiated hepatocyte function in vitro. Freshly isolated rat hepatocytes in co-culture with activated stellate cells rapidly aggregate to form well-defined viable spheroids. After 5 days in culture, the spheroids have a complex extracellular matrix support and hepatic ultrastructure including bile canaliculi, tight junctions, desmosomes and lipid storage. Co-culture spheroids have superior cytochrome P₄₅₀ (CYP₄₅₀) 3A and 2B function, and increased inducibility of 2B function, relative to a range of hepatocyte monoculture techniques (high-performance liquid chromatography of testosterone metabolites). Increased function in co-culture is supported by greater expression of CYP₄₅₀ 3A23, 1A2, and 2E1 mRNA relative to monoculture (reverse transcriptase quantitative polymerase chain reaction). Also, high hepatocyte growth factor mRNA expression in co-culture suggests a post-traumatic, or possibly regenerative, environment. A preliminary study of human hepatocytes co-cultured with rat stellate cells demonstrated prolonged function of CYP₄₅₀ 3A4, 2C19 and 2C9. This study shows that stellate cells facilitate spheroid formation, influence spheroid architecture, and are an effective method of preserving some aspects of hepatocyte function in the early stage of culture.

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A Study on the Mechanism of Intercellular Adhesion

Cited by 70 publications

References 52 publications

Effects of Anticonvulsant Drugs on Chick Embryonic Neurons and Glia in Cell Culture

Effects of Anticonvulsant Drugs on Chick Embryonic Neurons and Glia in Cell Culture

Two Mechanisms in Cell Adhesion Revealed by Effects of Divalent Cations

The Effect of Three-Dimensional Co-Culture of Hepatocytes and Hepatic Stellate Cells on Key Hepatocyte Functions in vitro

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