A study on the occurrence of aflatoxin M1 in milk consumed in Kosovo during 2009–2010

Rama, Adem; Montesissa, Clara; Lucatello, Lorena; Galina, Guglielmo; Benetti, C.; Bajraktari, D.

doi:10.1016/j.foodcont.2015.10.019

Cited by 16 publications

(2 citation statements)

References 17 publications

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“…In UHT milk, 7.2-9.9 ng/l AFM1 was detected. In this case, the environmental factors on AFM1 have been clearly demonstrated (Rama et al 2016).…”

Section: Discussionmentioning

confidence: 73%

“…In aflatoxin studies carried out in the world; the amount of aflatoxin may vary due to food type, climatic and regional factors. AFM1 level, the form of aflatoxin in the milk, is determined above the legal upper limits in our country and in the world regarding the presence of aflatoxin in milk (Rama et al 2015(Rama et al , 2016. In parallel with these studies, contamination sources causing contamination of aflatoxin in the milk, transmission route, abnormal state of contamination, etc.…”

Section: Introductionmentioning

confidence: 98%

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Investigation of the presence of Aspergillus Flavus in mastitis milk with PCR

Kırkan

Parın

Aycen

et al. 2019

Environ. Earth Ecol.

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In this study, milk samples were taken in sterile 20 ml containers from 90 cows with clinical mastitis in various enterprises around Aydın province between May-September 2017. The presence of Aspergillus flavus M1 toxin in milk samples was investigated by ELISA. Aflatoxin M1 ELISA results were compared with positive controls with 0 ppt, 5 ppt, 15 ppt, 30 ppt, 60 ppt and 100 ppt. For the 88 samples examined; 5 ppt in 7 (8,0 %), 15 ppt in 12 (13,6 %), 100 ppt in 32 (36,4 %) and 0 ppt in 37 (42,0 %) aflatoxin M1 was detected. According to these results, 32 (36,4 %) AFM1 positivity was detected in the European Union countries over the limit (50 ppt) accepted for raw milk. In our study, DNA obtained from 88 mastitis milk samples examined with Aflatoxin M1 ELISA kit was subjected to Aspergillus flavus species specific PCR process. As a result of PCR; Aflatoxin M1 at a level of 0 ppt in 7 (36,8 %), 5 ppt in 1 (% 5,2), 15 ppt in 2 (10,5 %) and 100 ppt in 9 (47,5 %) ELISA kit toxin presence was determined. As a result, 32 samples of 100 ppt AFM1 positivity were detected by ELISA and 9 samples 100 ppt positivity was detected by PCR in our study. The results of the ELISA test revealed that more positive results were formed due to cross-reactions, and the PCR method with FLA gene primers was more reliable for diagnosis.

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“…In UHT milk, 7.2-9.9 ng/l AFM1 was detected. In this case, the environmental factors on AFM1 have been clearly demonstrated (Rama et al 2016).…”

Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 98%

Investigation of the presence of Aspergillus Flavus in mastitis milk with PCR

Kırkan

Parın

Aycen

et al. 2019

Environ. Earth Ecol.

View full text Add to dashboard Cite

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Aflatoxin M1 contamination of raw cow’s milk in five regions of Kosovo during 2016

et al. 2018

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During February and August 2016 (winter and summer season), 192 samples of raw bulk tank milk have been collected from small dairy farms in five different regions of Kosova (Prishtina, Prizren, Peja, Mitrovica, and Gjilan). These samples were analyzed for aflatoxin M (AFM) contamination level by ultra-high performance liquid chromatography coupled with electrospray ionization triple quadrupole tandem mass spectrometry (UHPLC-ESI-MS/MS). The limit of quantification for AFM1 was 0.02 μg/L and recovery at 0.1-0.5 μg/L was 90-95% (coefficient of variation 15-25%). A total of 74 milk samples (38%) were found to be contaminated with AFM and 11 samples (5.7%) slightly exceeded the European Union maximum level of 0.05 μg/L. The maximum AFM level was at 0.082 μg/L. There was no relevant difference between summer and winter with regard to AFM frequency and levels. Although the frequency of AFM-positive samples varied between regions, from 7% (Prishtina) to 53% (Prizren), only minor regional differences were observed with regard to average and maximum toxin levels. The high percentage of milk samples which were non-compliant with AFM maximum levels indicates that efforts to reduce the contamination levels of aflatoxin B in cows feed in Kosovo are necessary.

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