Objectives:The purpose of the study was to examine the level and relationship of incivility through clinical experience and major satisfaction in dental hygiene students. Methods: A self-reported questionnaire was completed by 357 dental hygiene students in six universities from November 1 to December 1, 2015. The questionnaire by five point Likert scale consisted of general characteristics of the subjects (6 items), incivility (20 items), and major satisfaction (6 items). Incivility was divided into three factors of staff, doctors, and patients & visitors. Data were analyzed with descriptive statistics of variables, multiple response cross analysis, independent t-test, one way ANOVA, and Pearson's correlation coefficient analysis. Results: The mean of incivility and major satisfaction was 2.23 and 3.29, respectively. The incivility of staff, doctors, and patients & visitors showed negative correlations with major satisfaction. Conclusions: The incivility through clinical experience is associated with major satisfaction negative. These results suggest that we need to develop remedy such as positive attitude dealing with incivility and implementing similar environment education during clinical training at school and dealing with stress.