A study on β-glucan binding protein (β-GBP) and its involvement in phenoloxidase cascade in Indian white shrimp Fenneropenaeus indicus

Anjugam, Mahalingam; Vaseeharan, Baskaralingam; Iswarya, Arokiadhas; Amala, Muthu; Govindarajan, Marimuthu; Alharbi, Naiyf S.; Kadaikunnan, Shine; Khaled, Jamal M.; Benelli, Giovanni

doi:10.1016/j.molimm.2017.09.013

Cited by 15 publications

(5 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several previous studies demonstrate that βGRPs play a vital role in cellular immune response against fungal pathogens. The β-1,3-glucan binding proteins could agglutinate fungal cells and lead to prophenoloxidase activation (Fabrick et al, 2003;Sivakamavalli & Vaseeharan, 2014;Anjugam et al, 2017). Referring to these studies, binding interactions of TpβGRPs with microbial cells and probable molecular changes of signal pathways will be investigated further.…”

Section: Discussionmentioning

confidence: 99%

Characterization of novel β‐1,3‐glucan recognition proteins from a Tibetan Plateau ghost moth Thitarodes pui (Lepidoptera, Hepialidae)

Sun

Zhang

2018

Physiol. Entomol.

View full text Add to dashboard Cite

β‐1,3‐glucan recognition proteins (βGRPs) function as pattern recognition receptors in the innate immune response against invading pathogens. In the present study, we obtain full‐length cDNA clones for two novel putative βGRPs: TpβGRPc and TpβGRPd from the ghost moth Thitarodes pui (Lepidoptera: Hepialidae). Phylogenetic analysis shows a small distinct lineage, βGRP clade 4, consisting of T. pui βGRPs including TpβGRPa and TpβGRPb that have been identified previously. TpβGRPc and TpβGRPd, comprising 488 and 229 amino acids, have calculated molecular masses of 52 596 and 24 589 Da, respectively. TpβGRPc is 85.52% identical in sequence to TpβGRPa. TpβGRPb and TpβGRPd share the same deletion start site located at the conserved residue Pro 43, although TpβGRPd exhibits a much larger deletion of up to approximately 270 residues covering both the N‐ and C‐terminal regions. Affinity purification, associated with subsequent peptide sequencing, confirms the constitutive occurrence of TpβGRPa and TpβGRPc of similar size (approximately 65 kDa) in sixth‐instar larval haemolymph. These two βGRPs show clear binding affinities to curdlan, an insoluble β‐1,3‐glucan. A quantitative real‐time polymerase chain reaction analysis reveals the high‐level constitutive expression of TpβGRPc and TpβGRPd in the fat body of mid‐instar larvae, which are found to be susceptible to fungal pathogens in field investigations. Remarkable induction of both TpβGRPs occurs in response to haemocoelic challenge with entomopathogenic fungus Beauveria bassiana. The results of the present study suggest that TpβGRPs may contribute to the detection and control of fungal infections.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterization of novel β‐1,3‐glucan recognition proteins from a Tibetan Plateau ghost moth Thitarodes pui (Lepidoptera, Hepialidae)

Sun

Zhang

2018

Physiol. Entomol.

View full text Add to dashboard Cite

show abstract

“…PRRs have been identified in many insect species, including Drosophila melanogaster and Manduca sexta [14,15]. The most important PRRs purified from invertebrates include β-1,3 glucan binding protein (β-GBP), lipopolysaccharide and β-1,3 glucan binding protein (LGBP), Gram negative bacteria binding protein (G-NBP), peptidoglycan recognition protein (PGRP) and C-type lectin [16]. PGRPs and βGRPs are pattern recognition molecules that can recognize peptidoglycans in the bacterial cell wall and β-1,3-glucan from fungi, respectively, which play an important role in innate immunity activation and regulation [17,18].…”

Section: Introductionmentioning

confidence: 99%

Bombyx mori β-1,3-Glucan Recognition Protein 4 (BmβGRP4) Could Inhibit the Proliferation of B. mori Nucleopolyhedrovirus through Promoting Apoptosis

Wang

Zhu

et al. 2021

Insects

View full text Add to dashboard Cite

β-1,3-glucan recognition proteins (βGRPs) as pattern recognition receptors (PRRs) play an important role in recognizing various pathogens and trigger complicated signaling pathways in insects. In this study, we identified a Bombyx mori β-1,3-glucan recognition protein gene named BmβGRP4, which showed differential expression, from a previous transcriptome database. The full-length cDNA sequence was 1244 bp, containing an open reading frame (ORF) of 1128 bp encoding 375 amino acids. BmβGRP4 was strongly expressed in the larval stages and highly expressed in the midgut of B. mori larvae in particular. After BmNPV infection, the expression of BmβGRP4 was reduced significantly in the midgut. Furthermore, a significant increase in the copy number of BmNPV was observed after the knockdown of BmβGRP4 in 5th instar larvae, while the overexpression of BmβGRP4 suppressed the proliferation of BmNPV in BmN cells. Subsequently, the expression analysis of several apoptosis-related genes and observation of the apoptosis morphology demonstrated that overexpression of BmβGRP4 facilitated apoptosis induced by BmNPV in BmN cells. Moreover, BmβGRP4 positively regulated the phosphatase and tensin homolog gene (BmPTEN), while expression of the inhibitor of apoptosis gene (BmIAP) was negatively regulated by BmβGRP4. Hence, we hypothesize that BmNPV infection might suppress BmPTEN and facilitate BmIAP to inhibit cell apoptosis by downregulating the expression of BmβGRP4 to escape host antiviral defense. Taken together, these results show that BmβGRP4 may play a role in B. mori response to BmNPV infection and lay a foundation for studying its functions.

show abstract

“…Sequence similarities of 60%–71% have been noticed for M. rosenbergii and E. carinicauda LGBP with known crustacean LGBPs and ΒGBPs highlighting close family relationships under ΒGRPs 189,192 . Commonly, ΒGBPs elicit the activation of proPO after the degranulation of hemocytes 177,193 . However, the ΒGBP purified from crabs Portunus pelagicus, Paratelphusa hydrodromus and Scylla serrata also showed potent antibacterial activity against Gram‐positive bacteria, Gram‐negative bacteria and fungus, which was evaluated using the minimum inhibitory concentration (MIC) and anti‐biofilm activity assays 194–196 .…”

Section: Prrs In Crustaceansmentioning

confidence: 93%

“…189,192 Commonly, ΒGBPs elicit the activation of proPO after the degranulation of hemocytes. 177,193 However, the ΒGBP purified from crabs Portunus pelagicus, Paratelphusa hydrodromus and Scylla serrata also showed potent antibacterial activity against Gram-positive bacteria, Gram-negative bacteria and fungus, which was evaluated using the minimum inhibitory concentration (MIC) and anti-biofilm activity assays. [194][195][196] An antiviral function of ΒGBP is also suggested due to the abundance of transcript presence in the hepatopancreas of WSSV-resistant shrimp P. japonicus.…”

Section: βGrps Corresponds Propo Cascade Activation As Evidenced By Thementioning

confidence: 99%

The role of pattern recognition receptors in crustacean innate immunity

Patnaik

Baliarsingh

Sarkar³

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Crustacean species are among the most valued seafood with socioeconomic and ecological implications in the global fisheries sector. Microbial infections to the farming stock due to continued expansion and aquaculture puts global crustacean production at risk. Understanding and reviewing the complex innate immune system of crustaceans would be pivotal in formulating informed decisions for the control and management of epidemic diseases, thus making the global crustacean aquaculture industry sustainable. Under the purview of innate immunity in crustaceans, pattern recognition receptors (PRRs) represent the sensors capable of discriminating non‐self pathogenic modules and invoking the activation of immune responses. The immunity to infections can be manifested at cellular (via phagocytosis, apoptosis, nodulation and encapsulation) and humoral (effected by prophenoloxidase cascade, lectins, antimicrobial proteins) levels leading to the elimination of pathogens or subversion of the pathogenic implications. In this review, we discuss the participatory role of 12 types of PRRs identified from crustacean species with an exhaustive focus on structural and functional specificities in binding to pathogens and possible execution of the immune activity. Moreover, we have elaborated on the PRRs‐associated or possible signalling cascades (Toll‐NFκB, IMD‐NFκB and JAK‐STAT) in crustacean species that could bridge our understanding of the resistance or susceptibility of the host to varied pathogenic infections thus providing clues to the regulatory mechanisms involved in pathogenesis of infectious diseases and mechanisms of immunity.

show abstract

A study on β-glucan binding protein (β-GBP) and its involvement in phenoloxidase cascade in Indian white shrimp Fenneropenaeus indicus

Cited by 15 publications

References 49 publications

Characterization of novel β‐1,3‐glucan recognition proteins from a Tibetan Plateau ghost moth Thitarodes pui (Lepidoptera, Hepialidae)

Characterization of novel β‐1,3‐glucan recognition proteins from a Tibetan Plateau ghost moth Thitarodes pui (Lepidoptera, Hepialidae)

Bombyx mori β-1,3-Glucan Recognition Protein 4 (BmβGRP4) Could Inhibit the Proliferation of B. mori Nucleopolyhedrovirus through Promoting Apoptosis

The role of pattern recognition receptors in crustacean innate immunity

Contact Info

Product

Resources

About