This study designed three different types of sports bras based on the physiological characteristics of female upper bodies. The dressing pressure, skin temperature, and shaping support effectiveness of one subject when wearing those sports bras were evaluated under static and dynamic states. The results indicated that all designed bras illustrated excellent pressure comfort. Regarding the thermal comfort and shaping support performance, sports bras B2 and B3 with further optimizations had improved performances compared to B1. Besides, it was found that the skin temperature would increase with the dressing pressures from sports bras. The increased but comfortable dressing pressure could also benefit the shaping and support performance of sports bras. B22 and B33 had superior support performance to B1, with excellent ability to lift and gather the breasts, and to retract the waist. In summary, optimized sports bras with a cross, hollowed-out, and split design could effectively meet body comfort needs.