Abstract-In order to reducing the energy consumption of the train running between the stations, ensuring punctuality and the comfort of the passengers, this paper studies the train energyefficient operation strategy. After taking account of the slope and the speed limit of the line, the model of multi-objective optimization train energy-efficient is established based on train energy consumption, running time and passenger comfort. The improved multi-objective genetic algorithm (MOGA) is used to optimize the target speed sequence to obtain the operation strategy of the train. Different from previous multi-objective optimization, the energy-efficient driving optimization method is realized by considering automatic train operation's (ATO) double-level control structure, slope equivalent strategy, and Pareto optimization in this paper. Based on the actual line data and vehicle parameters of Yizhuang line in Beijing subway, the optimization method is verified by simulation. The simulation results show that, after using the improved multi-objective genetic algorithm, the energy consumption and running time of the train in Yizhuang train station are obviously decreased, and after the train comfort is measured, the rate of change in acceleration or deceleration meet the requirements of passenger experience needs. It can be seen that the proposed algorithm can effectively reduce the energy consumption of the train, ensure the accuracy of the running time and improve the comfort of the passengers.