A successful new approach to honeybee semen cryopreservation

Wegener, Jakob; May, Tanja; Kamp, Günter; Bienefeld, Kaspar

doi:10.1016/j.cryobiol.2014.07.011

Cited by 27 publications

(27 citation statements)

References 20 publications

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“…In the study, BSA5 group yielded higher motility rates than other groups at post thaw time point (P<0.05). The post-thaw motility values of drone semen cryopreserved with different extenders ranged between 25%-62% [8,11,12,24] . After thawing, the motility rate in BSA5 group has a common point with the findings of other studies.…”

Section: Discussionmentioning

confidence: 99%

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Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Alçay¹,

Çakmak²,

Çakmak³

et al. 2019

Kafkas Univ Vet Fak Derg

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The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x10 6 spermatozoon/mL. Motility, plasma membrane functional integrity (HOST), and defected acrosome (PSA-FITC) were evaluated in the study. At post thaw, the highest sperm motility rates were obtained in the BSA5 group (P<0.05). Functional integrity of sperm membrane was better preserved in the BSA3 and BSA5 groups compared to the other groups. The acrosomal integrity rates were higher in BSA5 group than in control group (P<0.05). The study shows that bovine serum albumin supplemented TL-Hepes based extenders have beneficial effect on drone semen parameters at post-thaw. The results of the study demonstrated a notable advantage of using 5 mg/mL of BSA in TL-Hepes based extender.

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Section: Discussionmentioning

confidence: 99%

“…Drone semen cryopreservation has been accomplished in recent years. However, the freezing success of drone semen has not reached the desired level nowadays [11,12] .…”

Section: Introductionmentioning

confidence: 99%

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Alçay¹,

Çakmak²,

Çakmak³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…In the more recent studies, sperm motility has been more frequently expressed as the percentage of motile cells, with some occasional indicators of the type of movement [20,25,74,89,94,95]. Spermatozoa may be classified as motile sperm if they present any type of active movement, freely motile if the sperm head shows displacement, and circular sperm if the sperm head and tail overlap [89].…”

Section: Sperm Motilitymentioning

confidence: 99%

“…These include the sperm chromatin structure assay (SCSA), the terminal transferase dUTP nick-end labelling (TUNEL) test, and the sperm chromatin dispersion (SCD) test. The TUNEL has been assayed in the honey bee to quantify DNA breakage caused by the cryopreservation procedure, although no clear increase was observed when compared to fresh semen samples [95]. Using the SCD test, a lower DNA fragmentation was observed in the sperm stored in the spermatheca than in the drone ejaculate [115].…”

Section: Dna Fragmentationmentioning

confidence: 99%

Sperm Quality Assessment in Honey Bee Drones

Yániz

Silvestre

Santolaria

2020

Biology

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The quality of honey bee drone semen is relevant in different contexts, ranging from colony productivity to pathology, toxicology and biodiversity preservation. Despite its importance, considerably less knowledge is available on this subject for the honey bee when compared to other domestic animal species. A proper assessment of sperm quality requires a multiple testing approach which discriminates between the different aspects of sperm integrity and functionality. Most studies on drone semen quality have only assessed a few parameters, such as sperm volume, sperm concentration and/or sperm plasma membrane integrity. Although more recent studies have focused on a broader variety of aspects of semen quality, some techniques currently used in vertebrates, such as computer-assisted sperm analysis (CASA) or multiparametric sperm quality testing, still remain to be developed in the honey bee. This may be attributed to the particular sperm morphology and physiology in this species, requiring the development of technologies specifically adapted to it. This article reviews the present knowledge of sperm quality in honey bee drones, highlighting its peculiarities and proposing future lines of research.

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“…Most studies into drone semen quality have only assessed a few parameters such as sperm volume, sperm concentration and/or sperm membrane integrity, which is also known as sperm viability (Collins and Pettis 2001;Lodesani et al 2004;Taylor et al 2009;Czekonska et al 2013b;Rousseau et al 2015;Ciereszko et al 2017). Some authors have investigated other parameters such as the assessment of certain molecules in sperm (Marti et al 1996;Wegener et al 2012;Ben Abdelkader et al 2014), the mitochondrial membrane potential (Ciereszko et al 2017), the proportion of DNA damage (Wegener et al 2014) and the effect different kinds of stress on spermatozoa (Nur et al 2012;Wegener et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of chamber characteristics, incubation, and diluent on motility of honey bee (Apis mellifera) drone sperm

2019

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In this study, we compared the effect of five different sample viewing devices (slide coverslips, Makler, Leja10, Leja20, and ISAS10 chambers), incubation time, analysis time, microscopic field analysis, and diluent used on honey bee semen motility parameters. Using media without proteins, a lower proportion of total motile and of freely motile sperm (those non-adhering to the glass surface) were observed for slide coverslip and slide coverslip-Makler chambers, respectively, than in other chambers, while the percentage of circular sperm followed an opposite trend. Significant increases in all motility parameters were observed when loaded Leja10 chambers were maintained at 35°C. During microscopic field analysis in the Leja Chamber, the percentage of freely motile sperm decreased and those of circular sperm increased in the last fields evaluated. The addition of 2% of BSA to the diluent clearly reduced the sperm adhesion to glass surface when using slide coverslip and Makler chambers. This study confirms that the choice of chamber and diluent used to assess honey bee drone sperm motility has a significant effect on the results wherein traditional slide coverslips are contraindicated.

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A successful new approach to honeybee semen cryopreservation

Cited by 27 publications

References 20 publications

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Sperm Quality Assessment in Honey Bee Drones

Effect of chamber characteristics, incubation, and diluent on motility of honey bee (Apis mellifera) drone sperm

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