2019
DOI: 10.9775/kvfd.2018.21311
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Arı Spermasının Protein Eklenmiş TL-Hepes Bazlı Sulandırıcı İle Dondurulması

Abstract: The aim of the current study was to determine the optimum concentration of bovine serum albumin for post-thawing quality of drone sperm and this is the first study to evaluate the effect of BSA supplemented TL-Hepes based extenders for drone semen cryopreservation. Sexually mature drones were used for semen collection. Pooled semen was diluted with TL-Hepes based extender supplemented with different concentrations of BSA (1 mg/mL, 3 mg/mL, and 5 mg/mL) or without BSA (control), at a final concentration of 100x… Show more

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“…Thus, the integrity and functional activity of the sperm membrane is of fundamental importance in the fertilization process, and assessment of membrane function may be a useful indicator of the fertilizing ability of spermatozoa [41]. The hypoosmotic swelling test (HOST) was used to evaluate the functional integrity of the sperm membrane for the spermatozoa of many species of domestic animals and humans, as well as honey bee drones [41,[65][66][67]. When exposed to hypo-osmotic conditions, water will enter the spermatozoon in an attempt to reach osmotic equilibrium.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, the integrity and functional activity of the sperm membrane is of fundamental importance in the fertilization process, and assessment of membrane function may be a useful indicator of the fertilizing ability of spermatozoa [41]. The hypoosmotic swelling test (HOST) was used to evaluate the functional integrity of the sperm membrane for the spermatozoa of many species of domestic animals and humans, as well as honey bee drones [41,[65][66][67]. When exposed to hypo-osmotic conditions, water will enter the spermatozoon in an attempt to reach osmotic equilibrium.…”
Section: Discussionmentioning
confidence: 99%