A suggested bisphenol A metabolite (MBP) interfered with reproductive organ development in the chicken embryo while a human-relevant mixture of phthalate monoesters had no such effects

Mentor, Anna; Bornehag, Carl‐Gustaf; Jönsson, Maria; Mattsson, Anna

doi:10.1080/15287394.2020.1728598

Cited by 10 publications

(12 citation statements)

References 83 publications

(118 reference statements)

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“…A total of 160 fertilized Leghorn eggs were obtained from the University of Delaware research farm and used in the assay. The chicken embryo as an in vivo model using early life stages has been widely used in toxicity assessment (Mentor, Bornehag, et al, 2020; Uggini et al, 2012; Zhang et al, 2021). The eggs were weighed and divided into 17 groups: vehicle control; two dosages of guaiacol, syringol, and three S/G mixtures (at ratio 0.5, 1, and 2); and three plant lignin monomers (MC, pine, and poplar).…”

Section: Methodsmentioning

confidence: 99%

The impact of differential lignin S/G ratios on mutagenicity and chicken embryonic toxicity

Zhang

Levia

Ebikade

et al. 2021

J of Applied Toxicology

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Lignin and lignin‐based materials have received considerable attention in various fields due to their promise as sustainable feedstocks. Guaiacol (G) and syringol (S) are two primary monolignols that occur in different ratios for different plant species. As methoxyphenols, G and S have been targeted as atmospheric pollutants and their acute toxicity examined. However, there is a rare understanding of the toxicological properties on other endpoints and mixture effects of these monolignols. To fill this knowledge gap, our study investigated the impact of different S/G ratios (0.5, 1, and 2) and three lignin depolymerization samples from poplar, pine, and miscanthus species on mutagenicity and developmental toxicity. A multitiered method consisted of in silico simulation, in vitro Ames test, and in vivo chicken embryonic assay was employed. In the Ames test, syringol showed a sign of mutagenicity, whereas guaiacol did not, which agreed with the T.E.S.T. simulation. For three S and G mixture and lignin monomers, mutagenic activity was related to the proportion of syringol. In addition, both S and G showed developmental toxicity in the chicken embryonic assay and T.E.S.T. simulation, and guaiacol had a severe effect on lipid peroxidation. A similar trend and comparable developmental toxicity levels were detected for S and G mixtures and the three lignin depolymerized monomers. This study provides data and insights on the differential toxicity of varying S/G ratios for some important building blocks for bio‐based materials.

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Section: Methodsmentioning

confidence: 99%

The impact of differential lignin S/G ratios on mutagenicity and chicken embryonic toxicity

Zhang

Levia

Ebikade

et al. 2021

J of Applied Toxicology

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“…In all chicken embryos (male and female), the same chemical induced abnormal Mullerian duct retention and altered gonadal morphology. Development of feminization in male gonads suggested an estrogenic mode of action produced by MBP, whereas the decrease in ovary size in females suggested an antiestrogenic mode of action. − It has also been reported that MBP had more potent estrogenic activity than parent BPA …”

Section: Common Endocrine-disrupting Chemicals (Edcs) and Evaluation ...mentioning

confidence: 99%

“…Sex-differentiation and reproductive organ development are similar in respect to cellular and molecular mechanisms between human and birds, which enable the chicken embryo to be a very useful tool for toxicity evaluation of EDCs. MBP(4-Methyl-2,4-bis(4-hydroxyphenyl) pent-1-ene), a major active BPA metabolite, upregulated female associated gonadal gene CYP19A1, FOXL2 and DMC1 expressed in male embryos and reduced expression of the testicular marker gene SOX9 . In all chicken embryos (male and female), the same chemical induced abnormal Mullerian duct retention and altered gonadal morphology.…”

Section: Common Endocrine-disrupting Chemicals (Edcs) and Evaluation ...mentioning

confidence: 99%

Use of Chicken Embryo Model in Toxicity Studies of Endocrine-Disrupting Chemicals and Nanoparticles

Ghimire

Zhang

et al. 2022

Chem. Res. Toxicol.

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Lab animals such as mice and rats are widely used in toxicity research of food additive and pharmaceutics, despite the wellrecognized research limitation such as the inability to simulate human neurological diseases, faster absorption of chemicals, big variations among species, and high cost when using a large number of animals. The Society of Toxicology's guidance now focuses on minimizing discomfort and distress of lab animals, finding alternative ways to reduce animal number, replacing animals with in vitro models, and complying to the animal welfare policies. The chicken embryonic model can be a better alternative to mice and rats because of its abundant availability and cost-effectiveness. It can be studied in both laboratory and natural environment, with easy manipulation in ovo or in vivo. The objective of this review paper is to evaluate the use of chicken embryonic model in toxicity evaluation for endocrine-disrupting chemicals (EDCs) and nanoparticles (NPs) by different end points to determine more comprehensive toxic responses. The end points include chicken embryonic mortality and hatchability, developmental malformation analysis, hormonal imbalance, physiological changes in endocrine organs, and antiangiogenesis. Major research methodologies using chicken embryos are also summarized to demonstrate their versatile practice and valuable application in modern toxicity evaluation of EDCs and NPs.

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“…There is a long tradition of using avian embryos to study sexual development and potential effects of environmental pollutants including EDCs ( Berg et al, 1998 ; Berge et al, 2004 ; Biau et al, 2007 ; Eising et al, 2001 ; Estermann, Major & Smith, 2021 ; Fry & Toone, 1981 ; Ioannidis et al, 2021 ). It is well known that the exposure of xenobiotics during avian embryonic development can induce irreversible deformities or malformations of the sex organs and disrupt sex-specific behavior ( Farhat et al, 2020 ; Guioli et al, 2020 ; Harnett et al, 2021 ; Mentor et al, 2020a ; Mentor et al, 2020b ; Ottinger et al, 2008 ; Quinn, Summitt & Ottinger, 2008 ; Sharin et al, 2021 ). One advantage is that the avian egg can be considered as a closed system lacking any exchange with its environment except for the interchange of gases.…”

Section: Introductionmentioning

confidence: 99%

No effects of the antiandrogens cyproterone acetate (CPA), flutamide and p,p’-DDE on early sexual differentiation but CPA-induced retardation of embryonic development in the domestic fowl (Gallus gallus domesticus)

Jessl,

Oehlmann

2023

PeerJ

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Because a wide range of environmental contaminants are known to cause endocrine disorders in humans and animals, in vivo tests are needed to identify such endocrine disrupting chemicals (EDCs) and to assess their biological effects. Despite the lack of a standardized guideline, the avian embryo has been shown to be a promising model system which responds sensitively to EDCs. After previous studies on the effects of estrogenic, antiestrogenic and androgenic substances, the present work focuses on the effects of in ovo exposure to p,p’-DDE, flutamide and cyproterone acetate (CPA) as antiandrogenic model compounds regarding gonadal sex differentiation and embryonic development of the domestic fowl (Gallus gallus domesticus). The substances were injected into the yolk of fertilized eggs on embryonic day one. On embryonic day 19 sex genotype and phenotype were determined, followed by gross morphological and histological examination of the gonads. Treatment with flutamide (0.5, 5, 50 µg/g egg), p,p’-DDE (0.5, 5, 50 µg/g egg) or CPA (0.2, 2, 20 µg/g egg) did not affect male or female gonad development, assessed by gonad surface area and cortex thickness in both sexes and by the percentage of seminiferous tubules in males as endpoints. This leads to the conclusion that antiandrogens do not affect sexual differentiation during embryonic development of G. gallus domesticus, reflecting that gonads are not target organs for androgens in birds. In ovo exposure to 2 and 20 µg CPA/g egg, however, resulted in significantly smaller embryos as displayed by shortened lengths of skull, ulna and tarsometatarsus. Although gonadal endpoints were not affected by antiandrogens, the embryo of G. gallus domesticus is shown to be a suitable test system for the identification of substance-related mortality and developmental delays.

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A suggested bisphenol A metabolite (MBP) interfered with reproductive organ development in the chicken embryo while a human-relevant mixture of phthalate monoesters had no such effects

Abstract: Mattsson (2020) A suggested bisphenol A metabolite (MBP) interfered with reproductive organ development in the chicken embryo while a human-relevant mixture of phthalate monoesters had no such effects,

Cited by 10 publications

References 83 publications

The impact of differential lignin S/G ratios on mutagenicity and chicken embryonic toxicity

The impact of differential lignin S/G ratios on mutagenicity and chicken embryonic toxicity

Use of Chicken Embryo Model in Toxicity Studies of Endocrine-Disrupting Chemicals and Nanoparticles

No effects of the antiandrogens cyproterone acetate (CPA), flutamide and p,p’-DDE on early sexual differentiation but CPA-induced retardation of embryonic development in the domestic fowl (Gallus gallus domesticus)

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