A Suggestion of Electrochemical Biosensor for Study of Platinum(II)‐DNA Interactions

Abstract

As a consequence of the employment of platinum based cytostatic drugs in tumor diseases treatment, it became necessary not only to detect them in biological samples but also to determine and study the Pt-DNA adducts, which is highly important during investigation of resistance on antitumor treatment. Thus, the main objective of this work was to study the interactions between DNA and cisplatin. Primarily, we studied changes in electrochemical behavior of cisplatin in the presence of different concentrations of sodium chloride using differential pulse voltammetry. We found out that the highest signal of cisplatin was measured in 0.75 M NaCl with detection limit of 100 pM. The current accepted opinion about platinum based cytostatics mechanism of action is that the drugs induce their cytotoxic properties through binding to the nuclear DNA. That is why we followed by investigation of DNA interactions with cisplatin. For these purposes we have suggested a new biosensor, EDTA metallothionein modified hanging mercury drop electrode (EDTA MT biosensor) and used cyclic voltammetry as a comparative technique. The average concentration of the drug bound to DNA was estimated as 8.1 ng of cisplatin per 500 ng of DNA by EDTA MT biosensor. It follows from the results obtained that the suggested EDTA MT biosensor could be a new useful tool for investigation of interaction of DNA with cisplatin, because give a response only in the present of Pt(II)-DNA adduct.

Determination of apo‐Metallothionein Using Adsorptive Transfer Stripping Technique in Connection with Differential Pulse Voltammetry

“…4C). In addition the limit quantification of apoMT was 100 pM at t A 500 s. Moreover, apoMT has been successfully used for suggestion of EDTA MT biosensor for determination of heavy metals [65]. We found out that the suggested biosensor had similar behavior as MT and/or PC biosensor, whereas the biological part of EDTA MT biosensor enabled to reach lower detection limits about 10 -30% [34,55].…”

“…It follows from the results obtained that EDTA can really unbound metal ions from MT, which means that apoMT is presented in this solution. This procedure could be an useful alternative tool for simple, rapid and undemanding preparation of apoMT to different purposes such as a suggestion of heavy metal biosensor [65].…”

Multi-instrumental Investigation of Affecting of Early Somatic Embryos of Spruce by Cadmium(II) and Lead(II) Ions

“…The labelling of signals is based on a number of papers published, which have been devoted to determination of thiols by the Brdicka reaction [16,17,39,[52][53][54][55][56][57][58][59][60][61][62][63] to structure of GSH -formation of RS2Co complex ( Figure 5B). The electrochemical behaviour of metallothionein measured by adsorptive transfer stripping technique in connection with different electrochemical methods is known [65][66][67][68][69], but the behaviour of such a small peptide as glutathione is not clear yet. As shown in Figure 5A and described by Vacek et al [15], GSH can be adsorbed on the surface of HMDE, and the modified electrode is placed in an electrochemical cell, where electrochemical analysis proceeds.…”

Caveolin-1 as a potential high-risk prostate cancer biomarker

“…Metallothioneins (MT) as a metal-binding proteins represent other promising tumour markers, which are intensely studied in connection with prostate carcinoma among other diseases (29)(30)(31). It has been recently demonstrated that metallothioneins play an important role in the development and progression of some tumour diseases (32)(33)(34)(35)(36)(37)(38)(39)(40)(41)(42).…”