Caveolin-1 as a potential high-risk prostate cancer biomarker

Gumulec, Jaromír; Sochor, Jiří­; Hlavna, Marián; Sztalmachová, Markéta; Křížková, Soňa; Babula, Petr; Hrabec, Roman; Rovný, Arne; Adam, Vojtěch; Eckschlager, Tomáš; Kizek, René; Masařík, Michal

doi:10.3892/or.2011.1587

Cited by 20 publications

(18 citation statements)

References 62 publications

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“…Thus, CAV-1 is considered to act as a tumour suppressor in the early stages of tumour development, whereas in more advanced stages it promotes tumorigenesis (Senetta et al, 2013). In prostate cancer, CAV-1 has been reported to be a potential highrisk marker (Gumulec et al, 2012). In the study presented here, an increase in the CAV-1 expression at the protein and mRNA levels was observed to be associated with the metastatic potential of the prostate cell lines.…”

Section: Discussionsupporting

confidence: 47%

Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Kowalska¹,

Nowakowska²,

Domińska³

et al. 2016

Acta Biochim Pol

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The aim of this study was to evaluate the coexpression of caveolin-1 (CAV-1), angiotensin II type 1 receptor (AT1-R) and forkhead box Ml (FOXM1) in prostate and breast cancer cell lines, in comparison with normal cell lines. CAV-1, AT1-R and FOXM1 expression was determined by reverse transcription-quantitative polymerase chain reaction and western blot analysis in the prostate cancer cell lines PC3, DU145 and LNCaP; prostate normal cell line PNT1A; breast cancer cell lines MCF-7 and MDA-MB-231; and the normal breast cell line 184A1. A correlation between the expression levels of the investigated genes and their metastatic properties was determined by the Spearman's rank test (P<0.05) and Aspin-Welsch t-test, respectively. In prostate cell lines, a significant correlation was noted between CAV-1 and AT1-R expression and between FOXM1 and CAV-1 expression. A correlation between the expression levels of the investigated genes and their metastatic potential was also observed, with relatively high expression of all the investigated genes in the normal prostate cell line PNT1A. In comparison to prostate cancer cell lines, an adverse dependency between CAV-1, AT1-R, FOXM1 expression and metastatic potential was observed in the breast cancer cell lines. Relatively high expression of all tested genes was observed in the normal breast cell line 184A1, which was decreasing respectively with increasing metastatic potential of breast cancer cell lines. The results obtained here indicate that CAV-1, FOXM1 and AT1-R may be potential markers of tumorigenesis in certain types of cancer in vitro.

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Section: Discussionsupporting

confidence: 47%

Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Kowalska¹,

Nowakowska²,

Domińska³

et al. 2016

Acta Biochim Pol

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“…These various effects may be explained by the activation status of different domains of Cav-1 or the expression levels of other molecules that interact with Cav-1 in these different signaling pathways [42]. Several studies have recently assessed the potential function of Cav-1 in tumor progression [8], [43], [44]. However, the relationship between Cav-1 expression and cancer progression remains unclear at both the cellular and molecular levels.…”

Section: Discussionmentioning

confidence: 99%

Loss of Stromal Caveolin-1 Expression: A Novel Tumor Microenvironment Biomarker That Can Predict Poor Clinical Outcomes for Pancreatic Cancer

Shan

Lü

et al. 2014

PLoS ONE

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AimsCancer development and progression is not only associated with the tumor cell proliferation but also depends on the interaction between tumor cells and the stromal microenvironment. A new understanding of the role of the tumor microenvironment suggests that the loss of stromal caveolin-1 (Cav-1) as a key regulator may become a potential therapy target. This study aims to elucidate whether stromal Cav-1 expression in pancreatic cancer can be a strong prognosis biomarker.MethodsTissue samples from 45 pancreatic cancer patients were studied. Parenchyma and stroma were separated and purified using laser capture microdissection. Stromal Cav-1 expression was measured from pancreatic cancer, paraneoplastic, and normal tissue using immunohistochemistry. We analyzed the correlation of stromal Cav-1 expression with clinicopathologic features and prognostic indicators, such as tumor marker HER-2/neu gene.ResultsSpecimens from six patients (13.3%) showed high levels of stromal Cav-1 staining, those from eight patients (17.8%) showed a lower, intermediate level of staining, whereas those from 31 patients (68.9%) showed an absence of staining. Cav-1 expression in cancer-associated fibroblasts was lower than that in paracancer-associated and in normal fibroblasts. Stromal Cav-1 loss was associated with TNM stage (P = 0.018), lymph node metastasis (P = 0.014), distant metastasis (P = 0.027), and HER-2/neu amplification (P = 0.007). The relationships of age, sex, histological grade, and tumor size with stromal Cav-1 expression were not significant (P>0.05). A negative correlation was found between circulating tumor cells and stromal Cav-1 expression (P<0.05).ConclusionThe loss of stromal Cav-1 in pancreatic cancer was an independent prognostic indicator, thus suggesting that stromal Cav-1 may be an effective therapeutic target for patients with pancreatic cancer.

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“…Herein, the membrane protein caveolin-1 (Cav1) came into focus as it is overexpressed or mutated in many solid human tumors67891011. Although Cav1 acts as tumor suppressor in non-transformed cells, its overexpression has been linked to tumor progression and poor prognosis12131415. As an example, overexpression of Cav1 has been identified as a marker for breast, lung and prostate cancer (PCa) progression that is associated with increased resistance to chemotherapy, metastatic disease and poor prognosis1617.…”

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Progression-related loss of stromal Caveolin 1 levels fosters the growth of human PC3 xenografts and mediates radiation resistance

Panic

Ketteler

Reis

et al. 2017

Sci Rep

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Despite good treatment results in localized prostate tumors, advanced disease stages usually have a pronounced resistance to chemotherapy and radiotherapy. The membrane protein caveolin-1 (Cav1) functions here as an important oncogene. Therefore we examined the impact of stromal Cav1 expression for tumor growth and sensitivity to ionizing radiation (IR). Silencing of Cav1 expression in PC3 cells resulted in increased tumor growth and a reduced growth delay after IR when compared to tumors generated by Cav1-expressing PC3 cells. The increased radiation resistance was associated with increasing amounts of reactive tumor stroma and a Cav1 re-expression in the malignant epithelial cells. Mimicking the human situation these results were confirmed using co-implantation of Cav1-silenced PC3 cells with Cav1-silenced or Cav1-expressing fibroblasts. Immunohistochemically analysis of irradiated tumors as well as human prostate tissue specimen confirmed that alterations in stromal-epithelial Cav1 expressions were accompanied by a more reactive Cav1-reduced tumor stroma after radiation and within advanced prostate cancer tissues which potentially mediates the resistance to radiation treatment. Conclusively, the radiation response of human prostate tumors is critically regulated by Cav1 expression in stromal fibroblasts. Loss of stromal Cav1 expression in advanced tumor stages may thus contribute to resistance of these tumors to radiotherapy.

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Caveolin-1 as a potential high-risk prostate cancer biomarker

Cited by 20 publications

References 62 publications

Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Coexpression of CAV-1, AT1-R and FOXM1 in prostate and breast cancer and normal cell lines and their influence on metastatic properties

Loss of Stromal Caveolin-1 Expression: A Novel Tumor Microenvironment Biomarker That Can Predict Poor Clinical Outcomes for Pancreatic Cancer

Progression-related loss of stromal Caveolin 1 levels fosters the growth of human PC3 xenografts and mediates radiation resistance

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