A sulfate-reducing bacterium with unusual growing capacity in moderately acidic conditions

Rampinelli, Letícia Roni; Azevedo, Roberta D’Angelo; Teixeira, Mônica Cristina; Guerra‐Sá, Renata; Leão, Versiane Albis

doi:10.1007/s10532-007-9166-y

Cited by 26 publications

(17 citation statements)

References 27 publications

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“…The sequences were then used for phylogenic analysis. The experimental procedures were fully described in Rampinelli et al (2008) and Rodrigues (2012).…”

Section: Bacterial Diversitymentioning

confidence: 99%

Comparison of Uasb and Fluidized-Bed Reactors for Sulfate Reduction

Bertolino

Silva

Aquino

et al. 2015

Braz. J. Chem. Eng.

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-Reactor hydrodynamics is important for sulfidogenesis because sulfate reduction bacteria (SRB) do not granulate easily. In this work, the sulfate reduction performance of two continuous anaerobic bioreactors was investigated: (i) an upflow anaerobic sludge blanket (UASB) reactor and (ii) a fluidized bed reactor (FBR). Organic loading, sulfate reduction, and COD removal were the main parameters monitored during lactate and glycerol degradation. The UASB reactor with biomass recirculation showed a specific sulfate reduction rate of 0.089±0.014 g.gSSV -1 .d -1 (89% reduction), whereas values twice as high were achieved in the FBR treating either lactate (0.200±0.017 g.gSSV -1 .d -1 ) or glycerol (0.178±0.010 g.gSSV -1 .d -1 ). Sulfate reduction with pure glycerol produced a smaller residual COD (1700 mg.L -1 ) than that produced with lactate (2500 mg.L -1 ) at the same COD.sulfate -1 mass ratio. It was estimated that 50% of glycerol degradation was due to sulfate reduction and 50% to fermentation, which was supported by the presence of butyrate in the FBR effluent. The UASB reactor was unable to produce effluents with sulfate concentrations below 250 mg.L -1 due to poor mixing conditions, whereas the FBR consistently ensured residual sulfate concentrations below such a value.

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“…The sequences were then used for phylogenic analysis. The experimental procedures were fully described in Rampinelli et al (2008) and Rodrigues (2012).…”

Section: Bacterial Diversitymentioning

confidence: 99%

Comparison of Uasb and Fluidized-Bed Reactors for Sulfate Reduction

Bertolino

Silva

Aquino

et al. 2015

Braz. J. Chem. Eng.

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“…Studies of salt marsh dissimilatory sulfite reductase genes (dsrAB), a highly conserved functional phylogenetic marker of prokaryotic sulfate reducers (49,57,102,103,107), have revealed both novel and deeply branching clades (3). Studies of mining-impacted sites at pH 2.0 to 7.8 (5,7,39,70,72,77,84), of soils and geothermal settings at a pH of ϳ4 (55, 68), of metal-contaminated estuaries at pH 6.8 to 7.2 (65), and of hypersaline lakes at pH 7.5 (56) further outline the distribution and tolerance of specific groups and species of SRB under geochemically stringent conditions. Other findings point toward the existence of deltaproteobacteria in environments at a pH of ϳ1 (10), although it is unknown if these include SRB.…”

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confidence: 99%

Diversity of Dissimilatory Sulfite Reductase Genes ( dsrAB ) in a Salt Marsh Impacted by Long-Term Acid Mine Drainage

Moreau

Zierenberg

Banfield

2010

Appl Environ Microbiol

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Sulfate-reducing bacteria (SRB) play a major role in the coupled biogeochemical cycling of sulfur and chalcophilic metal(loid)s. By implication, they can exert a strong influence on the speciation and mobility of multiple metal(loid) contaminants. In this study, we combined DsrAB gene sequencing and sulfur isotopic profiling to identify the phylogeny and distribution of SRB and to assess their metabolic activity in salt marsh sediments exposed to acid mine drainage (AMD) for over 100 years. Recovered dsrAB sequences from three sites sampled along an AMD flow path indicated the dominance of a single Desulfovibrio species. Other major sequence clades were related most closely to Desulfosarcina, Desulfococcus, Desulfobulbus, and Desulfosporosinus species. The presence of metal sulfides with low ␦ 34 S values relative to ␦ 34 S values of pore water sulfate showed that sediment SRB populations were actively reducing sulfate under ambient conditions (pH of ϳ2), although possibly within less acidic microenvironments. Interestingly, ␦ 34 S values for pore water sulfate were lower than those for sulfate delivered during tidal inundation of marsh sediments. 16S rRNA gene sequence data from sediments and sulfur isotope data confirmed that sulfur-oxidizing bacteria drove the reoxidation of biogenic sulfide coupled to oxygen or nitrate reduction over a timescale of hours. Collectively, these findings imply a highly dynamic microbially mediated cycling of sulfate and sulfide, and thus the speciation and mobility of chalcophilic contaminant metal(loid)s, in AMD-impacted marsh sediments.Salt marshes exhibit high primary production rates (1, 101) and form biogeochemical "transition zones" for nutrient production, transport, and cycling between terrestrial and coastal marine environments (41, 66, 100). These zones also serve to reduce the flux of potentially toxic metals in contaminated groundwater to estuaries (12,99,106). Both functions depend strongly on microbial activity, especially that of sulfate-reducing bacteria (SRB) (42,62,67). SRB recycle much of the sedimentary organic carbon pool in marsh sediments (42-44) and indirectly inhibit production of the greenhouse gas methane (37, 71). They can restrict the mobility of dissolved contaminant metals by inducing precipitation of poorly soluble metal sulfides, and studies have examined their use in constructed wetlands to bioremediate acid mine drainage (AMD) and other metalliferous waste streams (11,35,40,46,50,76,90,94,104). However, the high acidity and metal concentrations inherent to AMD can inhibit SRB growth (15,88,98), and preferential growth of iron-and sulfur-oxidizing bacteria over SRB has been observed in some treatment wetlands (39).For natural salt marshes, 16S ribosomal nucleic acid-and phospholipid fatty acid (PLFA)-based analyses have shown that SRB commonly comprise a significant fraction of the microbial community (13,24,31,34,51,58). Studies of salt marsh dissimilatory sulfite reductase genes (dsrAB), a highly conserved functional phylogenetic marker of pro...

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“…The optimum pH was 6.9, but it is worth noting that growth occurred over an unusually wide pH range (from pH 5.0 to 9.0). Because of H 2 S toxicity, growth of SRB at moderately acidic pH is uncommon, but has been reported recently (Rampinelli et al, 2008). For studies determining NaCl requirements, NaCl was weighed directly in the tubes at concentrations ranging from 0 to 5 % NaCl before dispensing a basal medium free from NaCl.…”

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confidence: 99%