Desulfocurvus vexinensis gen. nov., sp. nov., a sulfate-reducing bacterium isolated from a deep subsurface aquifer

Klouche, Nihel; Basso, Odile; Lascourrèges, Jean‐François; Cayol, Jean‐Luc; Thomas, Pierre; Fauque, Guy; Fardeau, Marie Laure; Magot, Michel

doi:10.1099/ijs.0.010363-0

Cited by 38 publications

(15 citation statements)

References 18 publications

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“…The metabolic provenance of several of the more abundant PLFAs is also still uncertain. A number of fatty acids have been linked, though not exclusively, to chemoautotrophy, such as 10-Me-16:0 (Desulfobacter or Desulfocurvus, sulfate reducers) and 18:1ω7 (Yamanaka and Sakata, 2004;Colaço et al, 2007;Klouche et al, 2009;Boschker et al, 2014), and their presence may be consistent with the hydrothermal signature of the sediment microbial community. There were notable proportions of compounds normally associated with sulfate-reducing bacteria (Kohring et al, 1994;Boschker et al, 2014).…”

Section: Microbial Signatures Of Hydrothermal Activitymentioning

confidence: 99%

Hydrothermal activity lowers trophic diversity in Antarctic hydrothermal sediments

et al. 2017

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Abstract. Hydrothermal sediments are those in which hydrothermal fluid is discharged through sediments and are one of the least studied deep-sea ecosystems. We present a combination of microbial and biochemical data to assess trophodynamics between and within hydrothermal and background areas of the Bransfield Strait (1050-1647 m of depth). Microbial composition, biomass, and fatty acid signatures varied widely between and within hydrothermally active and background sites, providing evidence of diverse metabolic activity. Several species had different feeding strategies and trophic positions between hydrothermally active and inactive areas, and the stable isotope values of consumers were not consistent with feeding morphology. Niche area and the diversity of microbial fatty acids was lowest at the most hydrothermally active site, reflecting trends in species diversity. Faunal uptake of chemosynthetically produced organics was relatively limited but was detected at both hydrothermal and non-hydrothermal sites, potentially suggesting that hydrothermal activity can affect trophodynamics over a much wider area than previously thought.

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Section: Microbial Signatures Of Hydrothermal Activitymentioning

confidence: 99%

Hydrothermal activity lowers trophic diversity in Antarctic hydrothermal sediments

et al. 2017

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“…The family Desulfovibrionaceae contains the genera Desulfovibrio (Kluyver and van Niel 1936;Skerman et al 1980) which is the type genus of the family (Kuever et al 2005b), Desulfobaculum (Zhao et al 2012), Desulfocurvus (Klouche et al 2009), Bilophila (Baron et al 1990(Baron et al , 1989, and Lawsonia (McOrist et al 1995). Gram-staining negative.…”

Section: Taxonomy Historical and Current Short Description Of The Fmentioning

confidence: 97%

“…The type strain of Desulfocurvus vexinensis, strain VNs36, was isolated with lactate from a well that collected water from a deep saline aquifer used for gas storage at a depth of 830 m in Paris Basin, France (Klouche et al 2009). …”

Section: Ecology Habitatmentioning

confidence: 99%

The Family Desulfovibrionaceae

Kuever¹

2014

The Prokaryotes

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“…However, despite these studies there is currently little understanding of the stress response mechanisms that may be utilized by microbial populations that are suddenly exposed to CO 2 at elevated pressures. Sulfate reducing bacteria (SRB) inhabit a wide range of diverse ecosystems, under conditions that are frequently considered to be “extreme” for microbial growth, including the deep subsurface in terrestrial and ocean environments (Bale et al, 1997; Sass and Cypionka, 2004; Basso et al, 2005; Klouche et al, 2009). Here we exposed a model subsurface SRB species, Desulfovibrio vulgaris , to CO 2 under a range of pressures, using a novel combination of high-pressure nuclear magnetic resonance spectroscopy (HP-NMR) and RNA-Seq to track both physiological and transcriptional responses to CO 2 stresses that may occur in deep subsurface injection sites.…”

Section: Introductionmentioning

confidence: 99%

CO2 exposure at pressure impacts metabolism and stress responses in the model sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough

et al. 2014

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Geologic carbon dioxide (CO2) sequestration drives physical and geochemical changes in deep subsurface environments that impact indigenous microbial activities. The combined effects of pressurized CO2 on a model sulfate-reducing microorganism, Desulfovibrio vulgaris, have been assessed using a suite of genomic and kinetic measurements. Novel high-pressure NMR time-series measurements using 13C-lactate were used to track D. vulgaris metabolism. We identified cessation of respiration at CO2 pressures of 10 bar, 25 bar, 50 bar, and 80 bar. Concurrent experiments using N2 as the pressurizing phase had no negative effect on microbial respiration, as inferred from reduction of sulfate to sulfide. Complementary pressurized batch incubations and fluorescence microscopy measurements supported NMR observations, and indicated that non-respiring cells were mostly viable at 50 bar CO2 for at least 4 h, and at 80 bar CO2 for 2 h. The fraction of dead cells increased rapidly after 4 h at 80 bar CO2. Transcriptomic (RNA-Seq) measurements on mRNA transcripts from CO2-incubated biomass indicated that cells up-regulated the production of certain amino acids (leucine, isoleucine) following CO2 exposure at elevated pressures, likely as part of a general stress response. Evidence for other poorly understood stress responses were also identified within RNA-Seq data, suggesting that while pressurized CO2 severely limits the growth and respiration of D. vulgaris cells, biomass retains intact cell membranes at pressures up to 80 bar CO2. Together, these data show that geologic sequestration of CO2 may have significant impacts on rates of sulfate reduction in many deep subsurface environments where this metabolism is a key respiratory process.

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Desulfocurvus vexinensis gen. nov., sp. nov., a sulfate-reducing bacterium isolated from a deep subsurface aquifer

Cited by 38 publications

References 18 publications

Hydrothermal activity lowers trophic diversity in Antarctic hydrothermal sediments

Hydrothermal activity lowers trophic diversity in Antarctic hydrothermal sediments

The Family Desulfovibrionaceae

CO2 exposure at pressure impacts metabolism and stress responses in the model sulfate-reducing bacterium Desulfovibrio vulgaris strain Hildenborough

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