A surface-enhanced Raman scattering aptasensor for ratiometric detection of aflatoxin B1 based on graphene oxide-Au@Ag core-shell nanoparticles complex

Chen, Peifang; Li, Chenbiao; Ma, Xiaoyuan; Wang, Zhouping; Zhang, Yin

doi:10.1016/j.foodcont.2021.108748

Cited by 36 publications

(11 citation statements)

References 47 publications

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“…In addition, CHM raw materials are susceptible to mycotoxin contamination during drying and storage, 94,95 such as aflatoxin B1 (AFB1), zearalenone (ZEN) and deoxynivalenol (DON). Although a variety of SERS active substrates, such as Au nanobipyramids, 96 silica-encapsulated hollow Au, 97 MXenes@Au dimers, 98 Au@Ag nanorods, 99 Au@Ag nanotriangles, 100 Au nanostar core-silver nanoparticle satellite assemblies, 101 and graphene oxide-Au@Ag, 102 have been widely used in the detection of mycotoxins, the sensitivity, repeatability and stability of the detection are still challenging. Recently, gold magnetic nanoparticles have become a promising SERS active substrate, [103][104][105] which can not only provide magnetic field regulation to improve repeatability, stability and sensitivity, but also provide aptamer modification sites to improve detection specificity.…”

Section: Contaminant Detectionmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy as a powerful method for the analysis of Chinese herbal medicines

Xu,

Song,

et al. 2024

Analyst

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Section: Contaminant Detectionmentioning

confidence: 99%

Surface-enhanced Raman spectroscopy as a powerful method for the analysis of Chinese herbal medicines

Xu,

Song,

et al. 2024

Analyst

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“…The recoveries of the sensor when applied to peanut samples were within the range of 92-103% with an RSD of 6-9%. [134] Although graphene has attracted more attention than any other 2D material, its simple chemistry and weak van der Waals bonding between layers in multilayer structures limit its use. Therefore, using complex or layered structures which contain two or more elements can offer new tunable properties arising from their compositional diversity and structural flexibility.…”

Section: Assembly Of 3d Nanosubstrates Using 2d Materialsmentioning

confidence: 99%

Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Logan,

Cao,

Freitag

et al. 2024

Advanced Materials

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The implementation of low‐cost and rapid technologies for the on‐site detection of mycotoxin‐contaminated crops is a promising solution to address the growing concerns of the agri‐food industry. Recently, there have been significant developments in Surface‐enhanced Raman spectroscopy (SERS) for the direct detection of mycotoxins in food and feed. This review provides an overview of the most recent advancements in the utilization of SERS through the successful fabrication of novel nanostructured materials. Various bottom‐up and top‐down approaches have demonstrated their potential in improving sensitivity, while many applications exploit the immobilization of recognition elements and molecular imprinted polymers (MIPs) to enhance specificity and reproducibility in complex matrices. Therefore, the design and fabrication of nanomaterials is of utmost importance and are presented herein. We uncover that limited studies establish limits of detection (LODs) or conduct validation using naturally contaminated samples. One decade on, SERS is still lacking significant progress and there is a disconnect between the technology, the European regulatory limits, and the intended end‐user. Ongoing challenges and potential solutions are discussed including nanofabrication, molecular binders, and data analytics. Recommendations to assay design, portability, and substrate stability are made to help improve the potential and feasibility of SERS for future on‐site agri‐food applications.This article is protected by copyright. All rights reserved

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“…Aptamer-based sensors (aptasensors) have been used in various areas, including protein detection, environmental analysis, biomedical analysis, and diagnostics [ 18 ]. Recently, some aptamers were used to detect AFB1 with lateral flow assay [ 19 ], surface-enhanced Raman scattering [ 20 ], electrochemical detection [ 21 ], and real-time quantitative PCR (qPCR) [ 22 ]. Although these assays are highly sensitive and specific, they cannot be used for on-site detection because the instruments are not portable [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

Aptamer against Aflatoxin B1 Obtained by SELEX and Applied in Detection

Yang

Tsai

2022

Biosensors

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Aflatoxins, especially aflatoxin B1 (AFB1), are the most prevalent mycotoxins in nature. They contaminate various crops and cause global food and feed safety concerns. Therefore, a simple, rapid, sensitive, and specific AFB1 detection tool is urgently needed. Aptamers generated by SELEX technology can specifically bind the desired targets with high affinity. The broad range of targets expands the scope of applications for aptamers. We used an AFB1-immobilized magnetic nanoparticle for SELEX to select AFB1-specific aptamers. One aptamer, fl−2CS1, revealed a dissociation constant (Kd = 2.5 μM) with AFB1 determined by isothermal titration calorimetry. Furthermore, no interaction was shown with other toxins (AFB2, AFG1, AFG2, OTA, and FB1). According to structural prediction and analysis, we identified a short version of the AFB1-specific aptamer, fl−2CS1/core, with a minimum length of 39-mer used in the AFB1-aptasensor system by real-time qPCR. The aptasensor showed a broad range of detection from 50 ppt to 50 ppb with an accuracy of 90% in the spiked peanut extract samples. With the application of the AFB1-aptasensor we have constructed, a wide range detection tool with high accuracy might be developed as a point-of-care testing tool in agriculture.

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A surface-enhanced Raman scattering aptasensor for ratiometric detection of aflatoxin B1 based on graphene oxide-Au@Ag core-shell nanoparticles complex

Cited by 36 publications

References 47 publications

Surface-enhanced Raman spectroscopy as a powerful method for the analysis of Chinese herbal medicines

Surface-enhanced Raman spectroscopy as a powerful method for the analysis of Chinese herbal medicines

Advancing Mycotoxin Detection in Food and Feed: Novel Insights from Surface‐Enhanced Raman Spectroscopy (SERS)

Aptamer against Aflatoxin B1 Obtained by SELEX and Applied in Detection

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