A survey of antibiotic-resistant bacteria in a sewage treatment plant in Thibodaux, Louisiana, USA

Everage, Travis J.; Boopathy, Raj; Nathaniel, Rajkumar; LaFleur, Gary; Doucet, John P.

doi:10.1016/j.ibiod.2014.05.028

Cited by 52 publications

(36 citation statements)

References 16 publications

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“…Pozo et al (2012) also found that the COD concentrations of wastewater treatment plants decreased by more than half by the time of discharge. The results from this study are inline with the results of Novo and Manaia (2010) and Everage et al (2014).…”

Section: Performance Of Sewage Treatment Plantsupporting

confidence: 88%

“…Different pure cultures isolated each month from the raw and treated sewage were subjected to antibiotic resistant assay. A bacterial lawn of the sample was grown on tryptic soy agar (TSA) media plate, using sterile cotton swabs as described by Everage et al (2014). After the sample was streaked onto the TSA plates, the antibiotic discs of erythromycin, tetracycline, and methicillin were placed using an automatic, hand-held disk dispenser.…”

Section: Antibiotic Resistant Testmentioning

confidence: 99%

“…A Fast ID DNA Extraction Kit was used according to manufacturer's instruction to extract the DNA. After the DNA was extracted, polymerase chain reaction (PCR) was used to amplify the DNA as described by Everage et al (2014). The presence of various antibiotic resistance genes was analyzed using the well known primers for methicillin (mecA gene), erythromycin (ermB gene), sulfonamides (sul1 gene), Tetracycline (tetA, tetW, and tetX genes for efflux pump, ribosomal protection, and enzymatic modification) as shown in Table 1 based on Burch et al (2013).…”

Section: Dna Extractionmentioning

confidence: 99%

“…One ml of this filtrate was added to 9 ml tryptic soy broth (TSB). A 0.2 ml of a lab strain culture of S. aureus (strain ATCC 25923, obtained from the American Type Culture Collection, Manassas, VA, USA) susceptible to methicillin and oxacillin was introduced to the TSB/filtrate mixture as described by Everage et al (2014). The culture was incubated at 37°C for 24 h. The culture was spread evenly across the surface of a Muller Hinton Agar plate and a methicillin antibiotic disc was placed on the top of the agar.…”

Section: Genetic Transformation Assaymentioning

confidence: 99%

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Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Naquin

Shrestha

Sherpa

et al. 2015

Bioresource Technology

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Section: Performance Of Sewage Treatment Plantsupporting

confidence: 88%

Section: Antibiotic Resistant Testmentioning

confidence: 99%

Section: Dna Extractionmentioning

confidence: 99%

Section: Genetic Transformation Assaymentioning

confidence: 99%

See 2 more Smart Citations

Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Naquin

Shrestha

Sherpa

et al. 2015

Bioresource Technology

Self Cite

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“…However, the effects of these trace contaminants are less clear (FattaKassinos et al, 2011;Vidal-Dorsch et al, 2012;Arpin-Pont et al, 2016) and impacts are only starting to emerge in the literature. For example, antibiotic resistant bacteria have been shown to evolve rapidly in areas subject to continual discharge of antibiotics, such as within wastewater treatment plants (WWTP) and even their immediate receiving environments (Rizzo et al, 2013;Everage et al, 2014;Manaia, 2014). Populations of fish exposed to even low concentrations of estrogenic compounds, have shown skewness in sex ratios and much higher incidents of intersex, among other problems (Vajda et al, 2008;Sumpter and Jobling, 2013).…”

Section: Introductionmentioning

confidence: 99%

Pharmaceuticals and Personal Care Products in Municipal Wastewater and the Marine Receiving Environment Near Victoria Canada

2017

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Concerns over the fate of pharmaceuticals and personal care products (PPCP) within the environment have been growing with the advent of more precise analytical methods. The discharge of municipal wastewater has been identified as a significant source of these contaminants, particularly where wastewater treatment is minimal. Here we present results from a comprehensive monitoring regime located around Victoria, BC, Canada. Samples were collected between 2009 and 2016 from a variety of sources including marine water, sediment and biota adjacent to two major untreated sewage outfalls, as well as the sewage itself. PPCP concentrations within the untreated sewage were high, and the sediment surrounding the outfalls showed corresponding contamination. However, this contamination dropped quickly with distance from the outfall such that by 800 m distance most PPCPs were below detection limits. Tissue samples of resident Northern Horse mussels (Modiolus modiolus) collected adjacent to one of the major sewage outfalls showed high single sample concentrations of the antimicrobial triclosan (317 ng g −1 dry weight), the antibiotic ciprofloxacin (176 ng g −1 dry weight), as well as the antidepressant sertraline (84.1 ng g −1 dry weight). Reference stations from around the region showed very low concentrations of contamination with almost all PPCP concentrations being below detection limits. Within the sewage, concentrations were largely stable overtime, with the exception of triclosan and triclocarban which both showed declines over the study period.

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A closer look at the antibiotic‐resistant bacterial community found in urban wastewater treatment systems

Tehrani

Gilbride

2018

MicrobiologyOpen

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The conventional biological treatment process can provide a favorable environment for the maintenance and dissemination of antibiotic‐resistant bacteria and the antibiotic resistance genes (ARG) they carry. This study investigated the occurrence of antibiotic resistance in three wastewater treatment plants (WWTP) to determine the role they play in the dissemination of ARGs. Bacterial isolates resistant to tetracycline were collected, and tested against eight antibiotics to determine their resistance profiles and the prevalence of multiple antibiotic resistance. It was found that bacteria resistant to tetracycline were more likely to display resistance to multiple antibiotics compared to those isolates that were not tetracycline resistant. Polymerase chain reaction (PCR) was used to identify the tetracycline resistance determinants present within the bacterial communities of the WWTPs and receiving waters, and it was found that ARGs may not be released from the treatment process. Identification of isolates showed that there was a large diversity of species in both the tetracycline‐resistant and tetracycline‐sensitive populations and that the two groups were significantly different in composition. Antibiotic resistance profiles of each population showed that a large diversity of resistance patterns existed within genera suggesting that transmission of ARG may progress by both horizontal gene and vertical proliferation.

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A survey of antibiotic-resistant bacteria in a sewage treatment plant in Thibodaux, Louisiana, USA

Cited by 52 publications

References 16 publications

Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Presence of antibiotic resistance genes in a sewage treatment plant in Thibodaux, Louisiana, USA

Pharmaceuticals and Personal Care Products in Municipal Wastewater and the Marine Receiving Environment Near Victoria Canada

A closer look at the antibiotic‐resistant bacterial community found in urban wastewater treatment systems

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