A survey of rotaviruses in sheep in Scotland

Dr, Snodgrass; Herring, JA; Ka, Linklater; Da, Dyson

doi:10.1136/vr.100.16.341-a

Cited by 7 publications

(7 citation statements)

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“…Their prevalence was lower than that reported by others, namely 20-60% in diarrhoeic lambs [2,4,31] and 30-60% in outbreaks of diarrhoea [4,7,32]. These data contrast with our previous observation of a high prevalence of antirotavirus antibodies in sheep sera in Spain [33].…”

Section: Clinical Characteristics Of the Outbreakscontrasting

confidence: 56%

Role of enteric pathogens in the aetiology of neonatal diarrhoea in lambs and goat kids in Spain

et al. 1996

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SUMMARYFaeces samples from diarrhoeic and non-diarrhoeic lambs and goat kids aged 1-45 days were examined for enteric pathogens. Cryptosporidium parvum was detected in both diarrhoeic lambs (45 %) and goat kids (42%) but not in non-diarrhoeic animals. F5+ (K99+) and/or F41+ Escherichia coli strains were isolated from 26% and 22 % of the diarrhoeic lambs and goat kids, respectively, although these strains, which did not produce enterotoxins ST I or LT I, were found with similar frequencies in non-diarrhoeic animals. A F5-F41 -ST I' E. coli strain was isolated from a diarrhoeic lamb (0-6%). Verotoxigenic E. coli was isolated from both diarrhoeic and non-diarrhoeic lambs (4-1 % and 8&2 %, respectively) and there was no association between infection and diarrhoea. The prevalence of group A rotavirus infection in diarrhoeic lambs was very low (2 1 %). Groups A and B rotaviruses were detected in three (8-1 %) and five (13-5 %) diarrhoeic goat kids from two single outbreaks. Group C rotaviruses were detected in four non-diarrhoeic goat kids. An association of diarrhoea and infection was demonstrated only for group B rotavirus. Clostridium perfringens was isolated from 10-8 % of the diarrhoeic goat kids but not from non-diarrhoeic goat kids or lambs. Salmonella arizonae was isolated from a diarrhoeic goat kid (2-7 %) and the clinical characteristics of the outbreaks where these two latter enteropathogens were found different from the rest. Picobirnaviruses were detected in a diarrhoeic lamb. No coronaviruses were detected using a bovine coronavirus ELISA. No evidence was found of synergistic effect between the agents studied. Enteric pathogens were not found in four (8-7 %) and three (20%) outbreaks of diarrhoea in lambs and goat kids, respectively.

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Section: Clinical Characteristics Of the Outbreakscontrasting

confidence: 56%

Role of enteric pathogens in the aetiology of neonatal diarrhoea in lambs and goat kids in Spain

et al. 1996

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“…The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutrMization. , Serological evidence of rotavirus infection in sheep has been reported (7,12,13). S~ODGnASS et al (10,12) and McNcLTY et al (5) have detected rotavirus particles in gut contents and feces of lamb with diarrhea by electron microscopy.…”

Section: Discussionmentioning

confidence: 94%

“…, Serological evidence of rotavirus infection in sheep has been reported (7,12,13). S~ODGnASS et al (10,12) and McNcLTY et al (5) have detected rotavirus particles in gut contents and feces of lamb with diarrhea by electron microscopy. They also reported that ovine rotavirus from fecal samples and from intestinal contents infected and synthesized viral antigen in lamb, pig and calf kidney cells, but the virus could not readily be subcultm'ed in these cells (5, 10, tl, 12).…”

Section: Discussionmentioning

confidence: 94%

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Isolation of ovine rotavirus in cell cultures

Makabe

Komaniwa

Kishi

et al. 1985

Archives of Virology

114

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Three cytopathic rotavirus strains were isolated in MA 104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.

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“…Rotaviral infections occur generally in young animals during the first few weeks of life. The infection has been observed in calves [38][39][40][41][42][43], pigs [44~46], foals [47], lambs [22,48,49], mice [50], rabbits [51,52], deer, mink and buffaloes [53]. In Scotland the infection appears to be widespread in sheep, in both the adults (38~,,) and in the lambs (56~o), with excretion of the virus in the feces of lambs possessing specific serum antibody [48].…”

Section: The Injectionmentioning

confidence: 99%