Isolation of ovine rotavirus in cell cultures

Abstract

Three cytopathic rotavirus strains were isolated in MA 104 cells from intestinal contents of lambs with diarrhea. Pretreatment of virus with pancreatin and incorporation of a small amount of pancreatin in maintenance medium were important for establishment of the strains in these cells. The isolates showed marked cross reactions with bovine rotavirus in immunofluorescence, but no cross reaction with bovine, human, simian, equine, porcine and lapine rotaviruses in neutralization.

Genomic characterization of a novel group A lamb rotavirus isolated in Zaragoza, Spain

“…Although group A lamb rotaviruses were first identified in the 1970s [3,[11][12][13][16][17][18] and a lamb rotavirus vaccine, containing OVR strain Lp14 (also known as Lanzhou Lamb Rotavirus [LLR]), is licensed for human use in China [45], the genotypic nature of OVRs circulating in diarrheic lambs is scanty because only a few OVR strains have been isolated and characterized to date. OVR strains have been isolated in the United Kingdom, Japan, and China, but only those isolated from the United Kingdom and China have been characterized [3,13,[16][17][18][19][20].…”

“…OVR strains have been isolated in the United Kingdom, Japan, and China, but only those isolated from the United Kingdom and China have been characterized [3,13,[16][17][18][19][20]. Indeed, the most well- [25] P [8] P [4] P [19] P [6] P [27] P [5] P [12] P [10] P [21] P[15] P [24] P [7] P [13] P [20] P [16] P [3] P [2] P [18] P [1] P [17] P [11] P [26] P [14] 0.05 nucleotide substitutions per bp A Fig. 4 The present study constitutes the first report of an OVR strain, 762, with P [14] and G8 specificities.…”

“…In the late 1970s, an OVR strain (K923), isolated from a diarrheic lamb, was used to study the pathogenicity of OVRs under experimental conditions using gnotobiotic lambs [12][13][14][15]. Subsequently, three additional OVR strains, L-1, L-2, and L-3, were isolated in Japan during an outbreak of diarrhea in a sheep farm [16,17]. The partially characterized OVR strain L-1 was shown to agglutinate erythrocytes and not antigenically related to G3, G4, G5, or G6 rotaviruses [16].…”

Replication of Atypical Ovine Rotavirus in Small Intestine and Cell Culture

“…In the present study several attempts to cultivate ovine virus in MA 104 and primary calf kidney cells, by protocols with and without trypsin, were initially unsuccessful and, although infected cells were eventually detected in MA 104 cultures, the virus could not be maintained by passage; a similar difficulty in adapting ovine group A rotavirus to cell culture has been reported (McNulty et al, 1976). Makabe et al (1985), however, have adapted ovine rotavirus to growth in MA104 cells by treatment with pancreatin rather than trypsin, but cytopathic changes and corresponding immunofluorescence were obtained only after four to six blind passages. The in vitro infection of cultured MA 104 cells with an atypical porcine rotavirus has recently been reported (Theil & Saif, 1985).…”

Hemagglutination with ovine rotavirus

“…Ovine rotavirus has been isolated in MA 104 cells, a stable cell line derived from embryonic rhesus monkey kidney, as one of the etiologic agents of lamb diarrhea (2). Recently we examind hemagglutination (HA) with ovine rotavirus grown in MA 104 cells and found that the virus agglutinated erythrocytes of chicken and some other animals.…”