Replication of Atypical Ovine Rotavirus in Small Intestine and Cell Culture

Chasey, D.; Banks, Jill

doi:10.1099/0022-1317-67-3-567

Cited by 10 publications

(4 citation statements)

References 16 publications

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“…Group A ORV strains isolated from lambs belong to serotypes G1, G3, G5, G6, G8, G9, and G10, among which G3, G6, and G10, with P types belonging to P[1], P [11] or P[14], are often encountered (Holland 1990;Munoz et al 1995a). ORV, isolated in MA104 cell lines can agglutinate chicken, sheep, rabbit, guinea pig and human erythrocytes (Chasey and Banks 1986). During earlier times, the detection of ORV depended on immunofluorescence or immuno-peroxidase test, and electron microscopy (Chasey and Banks 1986;Ellis and Daniels 1988), and by electron microscopy giant multinucleate syncytia composed of fused epithelial cells along with intracytoplasmic virus particles, are observed [Chasey and Banks 1986].…”

Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

“…ORV, isolated in MA104 cell lines can agglutinate chicken, sheep, rabbit, guinea pig and human erythrocytes (Chasey and Banks 1986). During earlier times, the detection of ORV depended on immunofluorescence or immuno-peroxidase test, and electron microscopy (Chasey and Banks 1986;Ellis and Daniels 1988), and by electron microscopy giant multinucleate syncytia composed of fused epithelial cells along with intracytoplasmic virus particles, are observed [Chasey and Banks 1986]. Latex agglutination test and ELISA have also been used to detect the presence of ORV in lambs (Ellis and Daniels 1988).…”

Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

“…Nowadays, nested reverse transcriptase-polymerase chain reaction (RT-PCR) are being developed for the detection, which is based on a target region in the sixth gene segment of ORV. For characterization of ORV at genomic level, PAGE pherotyping and gene sequencing are being commonly employed (Chasey and Banks 1986;Holland 1990). For prevention of the disease, the best option is feeding colostrum to the neonates so that passive immunity is transferred from dam to the young lambs.…”

Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

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Rotavirus diarrhea in bovines and other domestic animals

et al. 2008

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Rotavirus diarrhea is the major cause of death of millions of children in developing countries besides causing economically significant malady in neonates of many domestic animals. In neonates, the infection is non-viremic, have very short incubation period, and manifests profuse diarrhea and severe dehydration. Concurrent infection with secondary pathogens may augment the disease severity. Diarrhea occurs due to virus-mediated destruction of absorption efficient enterocytes, activation of enteric nervous system, or due to a rotavirus enterotoxin. Diagnosis of the infection relies on conventional techniques like isolation in MA 104 cell lines, electron microscopy, electro-pherotyping, and various serological tests. Presently, diagnosis and molecular typing is performed using serotype specific RT-PCR, sequencing or genomic hybridization techniques. As the rotaviruses are known to exhibit extreme genetic diversity and outplay disinfection procedures, eradication of the pathogen is often difficult. Hence, for prevention, good management practices coupled with vaccination of dam for protecting young ones, has to be practiced. Recently, new generation prophylactic strategies including DNA vaccines, subunit vaccines, virus-like particles (VLPs) and edible vaccines have been found to induce sufficient levels of passive immunity. Aside to the infection in animals, zoonotic significance of the animal rotaviruses has to be further unearthed. In this review, efforts have been made to highlight the importance and prevalence of the disease in bovines, its pathogenesis along with preventive measures, salient features of rotaviruses and their inter-species transmission abilities, zoonotic implications, and a concise account of the infection in various domestic animals and poultry.

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Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

Section: Disease In Other Domestic Animalsmentioning

confidence: 99%

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Rotavirus diarrhea in bovines and other domestic animals

et al. 2008

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“…A portion of each specimen was processed are often non-group A rotaviruses. 2,3,9 This report on group for cell culture immunofluorescent assay (CCIF) as described B rotavirus from diarrheic lambs in Ohio is the first on ovine previously 13 and then stored at 4 C until tested. The CCIF rotavirus in the United States.…”

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confidence: 99%