A survey of the Mycoplasma genitalium genome by using random sequencing

Peterson, Scott N.; Hu, Ping-Chuan; Bott, Kenneth F.; Hutchison, Clyde A.

doi:10.1128/jb.175.24.7918-7930.1993

Cited by 65 publications

(63 citation statements)

References 35 publications

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“…Similar projects in which large numbers of random clones were sequenced have been undertaken with Mycoplasma genitalium and have also yielded vast amounts of valuable information about the physiology and biochemistry of this organism (30). Very recently (31), these sequence-tagged sites have also been positioned on the genomic map, thus indicating the general utility of the genome scanning approach.…”

Section: Discussionmentioning

confidence: 98%

Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50

et al. 1995

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The physical map of the 3.6-megabase chromosome of Clostridium perfringens CPN50 was extended by positioning sites for the endonucleases SfiI and I-CeuI, and in parallel, the gene map was expanded by using a genome scanning strategy. This involved the cloning and sequencing of random chromosomal fragments, identification of the functions of the putative genes by database searches, and then hybridization analysis. The current gene map comprises almost 100 markers, many of which encode housekeeping functions while others are involved in sporulation or pathogenesis. Strikingly, most of the virulence genes were found to be confined to a 1,200-kb segment of the chromosome near oriC, while the pleiotropic regulatory locus, virRS, was situated toward the putative replication terminus. A comparison of the gene maps of three endospore-forming bacilli, C. perfringens, Clostridium beijerinckii, and Bacillus subtilis, revealed a similar order and distribution of key sporulation and heat shock genes which might reflect an ancient evolutionary relationship.Clostridium perfringens is a spore-forming, gram-positive anaerobe commonly found in the lower intestinal tracts of humans and other mammals as well as in soil and sewage. C. perfringens has been shown to cause a variety of diseases ranging in severity from the frequently fatal gas gangrene to a mild but common form of food poisoning (18,32). Clinical isolates can be classified into five serotypes, A to E, on the basis of their production of the four lethal typing toxins, the ␣-, ␤-, ε-, and -toxins (18,26,32). In addition to the typing toxins, most strains of C. perfringens produce a large variety of other toxins and hydrolytic enzymes, such as perfringolysin O, or -toxin, and collagenase, or -toxin, (23,25,33), that are likely to play a significant role in pathogenesis.A significant step forward in understanding the molecular genetics of this medically important anaerobe was made some years ago when a physical map of the chromosome of the paradigm strain of C. perfringens, CPN50, was constructed with six restriction enzymes and the positions of 28 genes were established (6). Subsequently, the extent of genomic diversity among the various serovars was assessed, three new markers were added, and the variation in the toxin gene repertoire was examined (9).Although a number of genes have been cloned recently from the clostridia, the rate of identification of new genetic markers has been relatively low. Consequently, in an attempt to rapidly expand the information content of the genome map, we have applied the technique of genome scanning in which random chromosomal fragments are cloned and sequenced and their putative functions are identified by means of database searches. These are then positioned on the physical map by means of hybridization. This strategy was particularly productive and not only led to the identification of 54 new loci but also generated a number of interesting leads for research in pathogenesis because several potential virulence genes were isolated. MATE...

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Section: Discussionmentioning

confidence: 98%

Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50

et al. 1995

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“…In M. genitalium, the smallest known genome, repeats of the three-gene operon that encodes one of the major surface proteins, the adhesin MgPa, represent more than 4% of the genome [8,24]. The percentage of identity between these repeats ranges from 78 to 90% [8], which is close enough for homologous recombination between the most similar regions, but far enough for complete gene conversion.…”

Section: Adaptation Of Pathogensmentioning

confidence: 99%

Functional and evolutionary roles of long repeats in prokaryotes

Rocha

Danchin

Viari³

1999

Research in Microbiology

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“…are reversed. In M. capicolum, the arrangement mpA-rpmHThe gyrB and gyrA genes in E. coli (16) and P. putida (21) are dnaA-dnaN is identical to that of other bacteria but the gyrB localized several kilobases apart on the chromosome, but in gene has not been identified (15 strain AA 2.2, B. burgdorferi, M. pneumoniae (24), and M. genitalium (22), gyrA and gyrB are coupled. DNA sequence analysis of the region downstream of the gyrB gene in M. hominis indicated that it was not coupled with gyrA.…”

Section: Atatactccaccatgtaattctttgatttgttttatcatgtcttctgtaatatctgctttmentioning

confidence: 99%

Sequencing analysis reveals a unique gene organization in the gyrB region of Mycoplasma hominis

Ladefoged

Christiansen

1994

J Bacteriol

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The homolog of the gyrB gene, which has been reported to be present in the vicinity of the initiation site of replication in bacteria, was mapped on the Mycoplasma hominis genome, and the region was subsequently sequenced. Five open reading frames were identified flanking the gyrB gene, one of which showed similarity to that which encodes the LicA protein of Haemophilus influenzae. The organization of the genes in the region showed no resemblance to that in the corresponding regions of other bacteria sequenced so far. The gyrA gene was mapped 35 kb downstream from the gyrB gene.

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A survey of the Mycoplasma genitalium genome by using random sequencing

Cited by 65 publications

References 35 publications

Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50

Rapid expansion of the physical and genetic map of the chromosome of Clostridium perfringens CPN50

Functional and evolutionary roles of long repeats in prokaryotes

Sequencing analysis reveals a unique gene organization in the gyrB region of Mycoplasma hominis

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