A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight japanese laboratories.

Matsuzawa, Toshiaki; Hayashi, Yutaka; Nomura, Mamoru; Unno, Takashi; Igarashi, Toshiji; Furuya, Tsuyoshi; Sekita, Kiyoshi; Ono, Atsushi; Kurokawa, Yuji; Hayashi, Yuzo

doi:10.2131/jts.22.25

Cited by 18 publications

(14 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The electrolyte concentrations in diabetic rat blood after the application of hemostatic dressing are given in Table . Normal electrolyte values obtained in literature were Na + 129.7 ± 11.3, K + 23.7 ± 11.51, Cl − 101.75 ± 3.59, Hct (%) 39.17 ± 2.78, and cHgb 14.77 ± 0.73 (g/dL) . Na + concentration in all the groups shows a rise compared to normal values.…”

Section: Resultsmentioning

confidence: 69%

“…Normal electrolyte values obtained in literature were Na 1 129.7 6 11.3, K 1 23.7 6 11.51, Cl 2 101.75 6 3.59, Hct (%) 39.17 6 2.78, and cHgb 14.77 6 0.73 (g/dL). 45 Na 1 concentration in all the groups shows a rise compared to normal values. This result can be interpreted that Na 1 ions from the used NaOH solution used for BC purification were released to the blood circulation.…”

Section: Diabetic Rat Injury Modelmentioning

confidence: 70%

See 1 more Smart Citation

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

et al. 2016

View full text Add to dashboard Cite

Patients with diabetes mellitus have an increased cardiovascular risk due to the abnormality of hemostatic system components. Therefore, hemostasis is an important concept when considering that diabetics are under risk due to potential bleeding complications during surgical operation. The aim of our study was to examine the efficiency of a fabricated nano/microbilayer hemostatic dressing for bleeding control in diabetic patients. For this purpose, we prepared a nano/microbilayer hemostatic dressing that has a porous sublayer, including chitosan (CTS), bacterial cellulose (BC) as basement and active agents in coagulation cascade, such as vitamin K (Vit K), protamine sulfate (PS), and kaolin (Kao) as a filler and an upper layer consisting of silk fibroin (SF) or SF/phosphatidylcholine (PC) blend to achieve complete hemostasis in diabetic rats. Coagulative performances of the prepared hemostatic dressings were examined by the determination of bleeding time, blood loss, and mortality rate through diabetic rat femoral artery injury model. The percent of diabetic rat blood absorption was found to be 247 ± 5% for gauze as opposed to 2214 ± 56% for SF-coated PS/BC/CTS. Vit K-reinforced within 138 s and SF-coated BC/CTS hemostatic dressings within 144 s showed a rapid coagulation time. In vivo coagulation studies demonstrated that hemostatic agent-reinforced BC/CTS hemostatic dressing, especially PS/BC/CTS showed a significant hemostatic plug formation. This study suggests that the high positive charge and porosity give to these hemostatic agents reinforced hemostatic dressings the ability to rapidly swell and to promote the accumulation of red blood cells and platelets through electrostatic interactions. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1573-1585, 2017.

show abstract

Section: Resultsmentioning

confidence: 69%

Section: Diabetic Rat Injury Modelmentioning

confidence: 70%

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

et al. 2016

View full text Add to dashboard Cite

show abstract

“…Blood analysis provided no sign for organ damage. GOT, GPT and ALP, all indicators of liver damage, and creatinine and urea, reflecting kidney function, were all within the normal range [45] in all groups. These findings were supported by organ weight analysis and histological examination of the liver and kidney, all of which were without pathological findings in all groups.…”

Section: Discussionmentioning

confidence: 65%

Safety Evaluation of a Bioglass–Polylactic Acid Composite Scaffold Seeded with Progenitor Cells in a Rat Skull Critical-Size Bone Defect

et al. 2014

View full text Add to dashboard Cite

Treating large bone defects represents a major challenge in traumatic and orthopedic surgery. Bone tissue engineering provides a promising therapeutic option to improve the local bone healing response. In the present study tissue biocompatibility, systemic toxicity and tumorigenicity of a newly developed composite material consisting of polylactic acid (PLA) and 20% or 40% bioglass (BG20 and BG40), respectively, were analyzed. These materials were seeded with mesenchymal stem cells (MSC) and endothelial progenitor cells (EPC) and tested in a rat calvarial critical size defect model for 3 months and compared to a scaffold consisting only of PLA. Serum was analyzed for organ damage markers such as GOT and creatinine. Leukocyte count, temperature and free radical indicators were measured to determine the degree of systemic inflammation. Possible tumor occurrence was assessed macroscopically and histologically in slides of liver, kidney and spleen. Furthermore, the concentrations of serum malondialdehyde (MDA) and sodium oxide dismutase (SOD) were assessed as indicators of tumor progression. Qualitative tissue response towards the implants and new bone mass formation was histologically investigated. BG20 and BG40, with or without progenitor cells, did not cause organ damage, long-term systemic inflammatory reactions or tumor formation. BG20 and BG40 supported bone formation, which was further enhanced in the presence of EPCs and MSCs.This investigation reflects good biocompatibility of the biomaterials BG20 and BG40 and provides evidence that additionally seeding EPCs and MSCs onto the scaffold does not induce tumor formation.

show abstract

“…Table shows the blood chemistry values after the created femoral artery bleeding model. Normal blood electrolyte values are 142–163 mmol Na + L −1 , 4.3–5.2 mmol K + L −1 , 0.27–1.26 mmol Ca ++ L −1 , 101–116 mmol Cl − L −1 , 13–27.1 mmol cTCO 2 L −1 , Hct (%) 36–44, 12.1–15.9 g cHgb dL −1 . The obtained electrolyte values are in normal range and the slight rise in Ca ++ electrolyte concentration is interpreted as Ca ++ ions released from the hemostatic dressings.…”

Section: Resultsmentioning

confidence: 89%

Hemostatic activities of nano/microporous bilayer dressings in a femoral artery bleeding rat model

Karahaliloğlu

Demirbilek

Ulusoy

et al. 2016

J of Applied Polymer Sci

View full text Add to dashboard Cite

Recently, an effective hemostatic dressing requirement has become a major problem in both the military and civilian world. Available hemostatic agents are too expensive, ineffective, unsafe, or complicated to use. Here, we evaluated the hemostatic efficacy of a nano/micro bilayer hemostatic dressing including a porous sublayer from chitosan (CTS) and bacterial cellulose (BC) and a nanofibrillar upper layer from silk fibroin (SF). In addition, several active agents rolled in coagulation cascade [vitamin K (Vit K), protamine sulfate (PS), kaolin (Kao), etc.] were doped to the sublayer of bilayer hemostatic agent and their activities were compared via in vivo and in vitro tests. Lactate dehydrogenase (LDH) activity test results demonstrated that BC/CTS, SF-coated BC/CTS, and Vit K K/BC/CTS, SF/phosphatidylcholine (PC)-coated BC/CTS showed higher LDH activity compared to standard gauze (p < 0.005). In a femoral artery bleeding rat model, SF-coated PS/BC/CTS significantly reduced bleeding time (80 6 0.3 s) compared to standard gauze and kaolin-doped group (p < 0.005). Blood loss and mortality rate with 2.3 g and 37.5% SF-coated BC/CTS and SF-coated PS/BC/CTS showed increased efficacy in achieving hemostasis compared to standard gauze. All the prepared hemostatic dressings except for kaolin-doped BC/CTS and standard gauze induced no inflammatory reaction in the tissue. All these data suggest that active agent-doped BC/CTS/SF-based bilayer hemostatic dressings have a great influence on the resulting hemostatic action. V C 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2016, 133, 43657.

show abstract

A survey of the values of clinical chemistry parameters obtained for a common rat blood sample in ninety-eight japanese laboratories.

Cited by 18 publications

References 16 publications

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

Active nano/microbilayer hemostatic agents for diabetic rat bleeding model

Safety Evaluation of a Bioglass–Polylactic Acid Composite Scaffold Seeded with Progenitor Cells in a Rat Skull Critical-Size Bone Defect

Hemostatic activities of nano/microporous bilayer dressings in a femoral artery bleeding rat model

Contact Info

Product

Resources

About