A survey of virus infections of the respiratory tract of cattle and their association with disease

Stott, E. J.; Thomas, Lewis H.; Collins, A. P.; Crouch, S.; Jebbett, Jean N.; Smith, Gary Scott; Luther, Peter; Caswell, Rachel J

doi:10.1017/s0022172400063294

Cited by 130 publications

(90 citation statements)

References 22 publications

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“…This supports the importance of BRSV as a cause of BRD. 2,17 Our simultaneous recovery of BRSV and other respiratory viruses (specifically BVDV and BHV-1) are interesting. These data may support speculation on the possible interaction or association between different respiratory viruses in the causation or enhancement of BRD.…”

Section: Discussionmentioning

confidence: 93%

“…2,17 The existence of the virus has been inferred since the late 1960's. 5 However, in the last decade the role of this agent as the cause of BRD became evident.…”

mentioning

confidence: 99%

“…5 However, in the last decade the role of this agent as the cause of BRD became evident. 2,17 The improvements in techniques for the diagnosis of BRSV have contributed to increased recognition. 1,2 Nevertheless, the accurate diagnosis of infections caused by this fastidious agent remains difficult.…”

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confidence: 99%

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Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

et al. 1989

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Abstract. Based on the marked antigenic similarities that exist between antigens of the human and bovine strains of respiratory syncytial virus (RSV), an enzyme immunoassay (EIA) designed to detect human RSV was used to detect bovine RSV. The commercial test kit (RSV EIA) consists of a solid phase (beads) coated with a capture antiserum prepared against the Long strain of human RSV.The RSV EIA test was compared with the method of inoculation of cell cultures and fluorescent antibody (FA) staining of lung tissue for the detection of bovine RSV. Using a cell culture-propagated stock of strain 375 of bovine RSV, the threshold of sensitivity of the EIA test for the cattle strain of RSV was determined to be ≤ 10 2,3 CCID 50 /ml. In addition, RSV EIA detected the bovine RSV in nasal samples obtained from 3 experimentally inoculated cattle. The RSV EIA exhibited a sensitivity of ≥ 80% during the period that shedding of infectious virus took place.All of the bovine RSV FA-positive lung samples (n = 37) were positive by the RSV EIA. Twenty-six of the remaining 214 bovine RSV FA-negative lung samples were positive by the RSV EIA. The RSV EIA was also used to test 137 nasal swabs obtained from cases of bovine respiratory disease. Of these, 38 tested positive by RSV EIA. All samples that tested positive by EIA were confirmed by blocking assays using hyperimmune serum anti-bovine RSV and a pool of monoclonal antibodies specific for that virus.

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Section: Discussionmentioning

confidence: 93%

“…2,17 The existence of the virus has been inferred since the late 1960's. 5 However, in the last decade the role of this agent as the cause of BRD became evident.…”

mentioning

confidence: 99%

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Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

et al. 1989

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“…BRSV infections cause considerable economic losses to the cattle industry in many parts of the world [2,19,20]. The cattle industry represents the major Uruguayan industry.…”

Section: Discussionmentioning

confidence: 99%

“…This infection represents an important economic loss to the cattle industry worldwide [2,19,20]. Recent studies have demonstrated a significantly higher milk production (1.39 kg more milk/day/cow) in dairy cows protected against BRSV [8].…”

Section: Introductionmentioning

confidence: 99%

Bovine respiratory syncytial virus: first serological evidence in Uruguay

et al. 2000

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-Bovine respiratory syncytial virus (BRSV) is a major cause of respiratory disease in calves resulting in a substantial economic loss for the cattle industry worldwide. In order to determine the presence of BRSV in Uruguay, an immunoenzymatic test was set up, using a recombinant BRSV nucleocapsid (N) protein as the antigen. The N protein was produced in Sf9 insect cells by a recombinant baculovirus expressing the N protein. Serum samples collected from one hundred cattle from four different geographic regions of Uruguay were analyzed. Antibodies against the N protein of BRSV were detected in 95% of the serum samples analyzed. These results show for the first time the presence of BRSV antibodies and suggest a widespread BRSV infection in the cattle population of Uruguay. respiratory syncytial virus / recombinant N protein / epidemiology / baculovirus / UruguayRésumé -Première mise en évidence sérologique du virus syncytial respiratoire bovin en Uruguay. Le virus syncytial respiratoire bovin (VSRB) est une cause majeure de maladie respiratoire chez le veau, provoquant des pertes économiques importantes pour l'industrie bovine dans le monde entier. Afin de mettre en évidence la présence du VSRB en Uruguay, un test immunoenzymatique a été élaboré, qui utilise comme antigène une protéine recombinante de nucléocapside (N) du VSRB. La protéine N a été produite dans des cellules d'insecte Sf9 par un baculovirus recombinant exprimant la protéine N. Des échantillons de sérum prélevés sur 100 bovins, provenant de quatre régions différentes d'Uruguay, ont été analysés. Des anticorps dirigés contre la protéine N du VSRB ont été détectés dans 95% des échantillons analysés. Ces résultats montrent, pour la première fois, la présence d'anticorps anti-VSRB et suggèrent que l'infection par le VSRB est largement répandue dans la population bovine en Uruguay. virus syncytial respiratoire bovin / protéine N recombinante / épidémiologie / baculovirus / Uruguay Vet. Res. 31 (2000) [241][242][243][244][245][246] 241

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Picornaviruses

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2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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A survey of virus infections of the respiratory tract of cattle and their association with disease

Cited by 130 publications

References 22 publications

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

Detection of Bovine Respiratory Syncytial Virus using a Heterologous Antigen-Capture Enzyme Immunoassay

Bovine respiratory syncytial virus: first serological evidence in Uruguay

Picornaviruses

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