A systematic assessment of<i>Morchella</i>using RFLP analysis of the 28S ribosomal RNA gene

Bunyard, Britt A.; Nicholson, Michael S.; Royse, Daniel J.

doi:10.1080/00275514.1994.12026481

Cited by 82 publications

(32 citation statements)

References 30 publications

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“…DNA was extracted and amplified using a REDExtract-N-Amp Plant PCR Kit (Sigma-Aldrich, USA), following the manufacturer's protocol except that the ascomata were ground in 30 μL extraction solution with a plastic pestle. Amplification primers for ITS were ITS1F and ITS4 (White et al 1990, Gardes & Bruns 1993, for LSU were LR0R and LR5 (Vilgalys & Hester 1990, Bunyard et al 1994. The DNA sequences from the all three fruiting bodies and from the culture were identical and have been accessioned in Genbank as KU131677 and KU131678.…”

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Neocoleroa metrosideri sp. nov. (Sympoventuriaceae, Venturiales)

Johnston

Park

2016

Phytotaxa

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Neocoleroa metrosideri is described as a new species and phylogenetically it is shown to belong in the Sympoventuriaceae, a recently established family, sister to the Venturiaceae. No other sequences are available for Neocoleroa but this new species is morphologically typical of the type species, with distinctive lobed to dichotomously branched, blunt-tipped setae on the superficial pseudothecia. The genus has previously been placed in the Pseudoperisporiaceae. This leaf spotting fungus is known from only a single specimen in an urban park but the same fungus has been detected also from two natural forest sites as an OTU in 454-based high throughput amplicon sequencing from DNA extracted from living leaves of Metrosideros excelsa.Keywords: LSU phylogeny, Metrosideros excelsa, New Zealand, Wentiomyces, 454 high throughput sequencingThe genera Neocoleroa Petrak (1934: 38) and Wentiomyces Koorders (1907: 168) have had a tangled taxonomic history. Kirk et al. (2008) Barr (1987, as Dimeriaceae) noted that some of these fungi are morphologically close to the Venturiaceae. This paper describes a new species Neocoleroa metrosideri, associated with leaf spots of Metrosideros excelsa. Morphologically it fits Barr's concept of Neocoleroa. DNA sequences from the type specimen and from cultures grown from single ascospores from the type, place this species in the Sympoventuriaceae, sister to the Venturiaceae in the Venturiales (Zhang et al. 2011). Materials and MethodsThe type specimen was examined when fresh, hymenial elements mounted in water; intact pseudothecia and adjacent host leaf tissues were rehydrated in 3% KOH and vertical sections were cut at a thickness of about 10 μm using a freezing microtome and mounted in lactic acid. Mature pseudothecia were crushed gently in 1% streptomycin solution, released ascospores streaked across a water agar plate and after 24 h single germinating ascospores transferred to Difco PDA, 2% Difco MEA, and Difco oatmeal agar plates. Cultures were described after 4 weeks. Specimens have been deposited in the PDD fungarium and ICMP culture collection.For DNA extraction, three separate extractions were done from single ascomata from three different leaves from PDD 107531 and from a culture derived from germinated ascospores from an ascoma from the collection. DNA was extracted and amplified using a REDExtract-N-Amp Plant PCR Kit (Sigma-Aldrich, USA), following the manufacturer's protocol except that the ascomata were ground in 30 μL extraction solution with a plastic pestle. Amplification primers for ITS were ITS1F and ITS4 (White et al. 1990, Gardes & Bruns 1993, for LSU were LR0R and LR5 (Vilgalys & Hester 1990, Bunyard et al. 1994. The DNA sequences from the all three fruiting bodies and from the culture were identical and have been accessioned in Genbank as KU131677 and KU131678.Additional LSU sequences were downloaded from Genbank, the taxon selection based on Machouart et al. (2014). LSU NEOCOLEROA METROSIDERI SP. NOV. Phytotaxa 253 (3) © 2016 Magnolia Press • 215sequences were a...

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Neocoleroa metrosideri sp. nov. (Sympoventuriaceae, Venturiales)

Johnston

Park

2016

Phytotaxa

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“…Most of them were described from Europe, with only few described in Asia and USA. Due to insufficient microscopic characteristics and high levels of variability in form and color of ascocarps during different developmental stages (Du et al 2014), affected by ecological and climate factors, the species number in Morchella varies from 3 to 50 or more, which has caused confusing use of homonyms and synonyms (Bresinsky et al 1972; Gessner et al 1987; Volk and Leonard 1989; Jung et al 1993; Bunyard et al 1994, 1995; Kanwal et al 2011; Clowez 2012; Kuo et al 2012; Mortimer et al 2012; Richard et al 2014). …”

Section: Species Diversity In Morchellamentioning

confidence: 99%

A review on research advances, issues, and perspectives of morels

Zhao

Yang

2015

Mycology

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Morels, a group of the world’s most prized edible and medicinal mushrooms, are of very important economic and scientific value. Here, we review recent research progress in the genus Morchella, and focus on its taxonomy, species diversity and distribution, ecological diversity, phylogeny and biogeography, artificial cultivation, and genome. We also discuss the potential issues remaining in the current research and suggest some future directions for study.

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“…Anderson and Stasovski (1992) determined the molecular phylogeny of species of Armillaria from the Northern Hemisphere using sequence analysis following polymerase chain reaction (PCR) amplification of the intergenic region between the 26S and the 5S rRNA genes. Other researchers have used RFLP analysis of PCRamplified rDNA to distinguish isolates of the Gaeumannomyces-Phialophora complex of fungi (Ward and Akrofi, 1994), strains of Rhizobium (Laguerre et al, 1994), genotypes of Pleurotus (Bunyard et al, 1996), and species of Morchella (Bunyard et al, 1995(Bunyard et al, , 1994.…”

Section: Introductionmentioning

confidence: 99%

“…These variations are used to determine relatedness between species, as well as among a single species. The use of RFLP analysis (Bunyard et al, 1996(Bunyard et al, , 1994Cubeta et al, 1991;Hibbett and Vilgalys, 1991;Kohn et al, 1988;Magee et al, 1987) and direct sequencing (White et al, 1990;Medlin et al, 1988;Woese and Olsen, 1986) to investigate the genes coding for the production of 16S-18S, 5.8S, 26S-28S, and 5S ribosomal RNA are well established methods for the assessment and comparison of phylogenetic relationships of organisms (Sogin, 1990) over a wide range of taxonomic levels. The rRNA genes of fungi are located on a single chromosome and are present as repeated subunits of a tandem array of transcribed and nontranscribed stretches of DNA (Cassidy et al, 1984;Petes and Botstein, 1977).…”

Section: Introductionmentioning

confidence: 99%