Cultural ecosystem services (CES) have been proposed as the "intangible and non-material benefits that people enjoy from ecosystems", and the literature has been expanding on how CES are defined, identified, valued, and incorporated into policy. However, the literature on CES has a strong geographical bias toward Europe and North America. In this Special Feature, authors examine how and in what ways CES concepts and frameworks have applicability in diverse developing country settings, and the particular challenges that CES approaches face. By looking at CES across different contexts in the global South, the articles emphasize the usefulness of a range of methodologies for eliciting and valuing CES; the importance of CES for a variety of people, including urban dwellers and Indigenous peoples; and the need for more practices and programs for ecosystem management that incorporate CES. Overall, the articles in this Special Feature show that research focusing on the global South can make positive contributions to the growing CES literature by drawing attention to key challenges such as power and inequality in access to CES, pressures from social and environmental change on CES, and the importance of relational and other culturally diverse values elicited through appropriate methodologies.