A Systematic Nomenclature for the Drosophila Ventral Nerve Cord

Court, Robert; Namiki, Shigehiro; Armstrong, J. D.; Börner, Jana; Card, Gwyneth M; Costa, Marta; Dickinson, Michael H.; Duch, Carsten; Korff, Wyatt; Mann, Richard S.; Merritt, David J.; Murphey, Rod K.; Seeds, Andrew M.; Shirangi, Troy R.; Simpson, Jeffrey H.; Truman, James W.; Tuthill, John C.; Williams, Darren W.; Shepherd, David

doi:10.1016/j.neuron.2020.08.005

Cited by 73 publications

(89 citation statements)

References 57 publications

(70 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Image data for light level type example skeletons from FlyCircuit for previously described types (15) can be browsed by searching for the neuron identifier at http://www.virtualflybrain.org/. Neuropil nomenclature was based on (18) for the brain, and (60) for the VNC.…”

Section: Methodsmentioning

confidence: 99%

“…A similar template was derived from the nc82 expression pattern in the VNC of an example female Canton S fly imaged by the FlyLight Project team (template available here: https://github.com/VirtualFlyBrain/DrosAdultVNSdomains/blob/master/template/Neuropil_185.nrrd). Our VNC alignment pipeline was adapted from (60). Briefly: confocal VNC stacks were first converted to an 8-bit nrrd file format, preprocessed using the nc82 reference channel to normalize contrast across samples, rotated to approximately orient the VNC along the anterior-posterior axis, and then the channels were aligned to the template by nonrigid warping (62) using the Computational Morphometry Toolkit.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output

Huoviala

Dolan

Love

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

Evolution has tuned the nervous system of most animals to produce stereotyped behavioural responses to ethologically relevant stimuli. For example, female Drosophila avoid laying eggs in the presence of geosmin, an odorant produced by toxic moulds.Using this system, we now identify third order olfactory neurons that are essential for an innate aversive behaviour. Connectomics data place these neurons in the context of a complete synaptic circuit from sensory input to descending output. We find multiple levels of valence-specific convergence, including a novel form of axo-axonic input onto second order neurons conveying another danger signal, the pheromone of parasitoid wasps. However we also observe a massive divergence as geosmin-responsive second order olfactory neurons connect with a diverse array of ~75 cell types. Our data suggest a transition from a labelled line organisation in the periphery to one in which olfactory information is mapped onto many different higher order populations with distinct behavioural significance.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output

Huoviala

Dolan

Love

et al. 2018

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Brain and VNC anatomical axes are presented on lower left of first brain and VNC image panels: D, dorsal ; M, medial ; A, anterior ; P, posterior ; V, ventral . Further information on brain and VNC position and structure has been previously described [ 90 , 106 ]. Scale bars = 50 μm.…”

Section: Supporting Informationmentioning

confidence: 99%

“…Brain anatomical axes are presented on lower left of first brain image panel (see E): D, dorsal; M, medial; A, anterior; P, posterior. For Chromatag transgene expression at other time points in this study see S1 Fig.Furtherinformation on the Drosophila central nervous system, including anatomical structures and nomenclature, has been previously described[90,106]. (E-F") Flies are the genotype: w/Y; P[w +mC , UAS-GAL4]/P[w +mC , UAS-Chromatag]; fru P1-Gal4/+.…”

mentioning

confidence: 99%

Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior

et al. 2021

View full text Add to dashboard Cite

Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examiningDrosophila melanogaster fru P1neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluate five chromatin modifications, at three life stages in both sexes. We find substantial changes in chromatin modification profiles across development and fewer differences between males and females. Additionally, we find chromatin modifications that persist in different sets of genes from pupal to adult stages, which may point to genes important for cell fate determination infru P1neurons. We generated cell-type-specific RNA-seq data sets, using translating ribosome affinity purification (TRAP). We identify actively translated genes infru P1neurons, revealing novel stage- and sex-differences in gene expression. We also find chromatin modification enrichment patterns that are associated with gene expression. Next, we use the chromatin modification data to identify cell-type-specific super-enhancer-containing genes. We show that genes with super-enhancers infru P1neurons differ across development and between the sexes. We validated that a set of genes are expressed infru P1neurons, which were chosen based on having a super-enhancer and TRAP-enriched expression infru P1neurons.

show abstract

“…Given that insects are a diverse and large group of animals within the Arthropoda phylum, we will limit ourselves to discussing only Drosophila , as it is the most common insect experimental model in neuroscience. The Drosophila nervous system consists of a longitudinally running ventral nerve cord (VNC) located in the ventral thorax and an anterior bulbous brain within the head ( Court et al, 2020 ). For those more familiar with vertebrate nervous systems, the VNC is often likened to the spinal cord.…”

Section: Do Insects Possess the Neural Architecture Necessary For Pain?mentioning

confidence: 99%

Neural Design Principles for Subjective Experience: Implications for Insects

Key

Zalucki

Brown

2021

Front. Behav. Neurosci.

View full text Add to dashboard Cite

How subjective experience is realized in nervous systems remains one of the great challenges in the natural sciences. An answer to this question should resolve debate about which animals are capable of subjective experience. We contend that subjective experience of sensory stimuli is dependent on the brain’s awareness of its internal neural processing of these stimuli. This premise is supported by empirical evidence demonstrating that disruption to either processing streams or awareness states perturb subjective experience. Given that the brain must predict the nature of sensory stimuli, we reason that conscious awareness is itself dependent on predictions generated by hierarchically organized forward models of the organism’s internal sensory processing. The operation of these forward models requires a specialized neural architecture and hence any nervous system lacking this architecture is unable to subjectively experience sensory stimuli. This approach removes difficulties associated with extrapolations from behavioral and brain homologies typically employed in addressing whether an animal can feel. Using nociception as a model sensation, we show here that the Drosophila brain lacks the required internal neural connectivity to implement the computations required of hierarchical forward models. Consequently, we conclude that Drosophila, and those insects with similar neuroanatomy, do not subjectively experience noxious stimuli and therefore cannot feel pain.

show abstract

A Systematic Nomenclature for the Drosophila Ventral Nerve Cord

Cited by 73 publications

References 57 publications

Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output

Neural circuit basis of aversive odour processing in Drosophila from sensory input to descending output

Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior

Neural Design Principles for Subjective Experience: Implications for Insects

Contact Info

Product

Resources

About