A Systematic Nomenclature for the<i>Drosophila</i>Ventral Nervous System

Court, Robert; Armstrong, J. D.; Börner, Jana; Card, Gwyneth M; Costa, Marta; Dickinson, Michael H.; Duch, Carsten; Korff, Wyatt; Mann, Richard S.; Merritt, David J.; Murphey, Rod K.; Namiki, Shigehiro; Seeds, Andrew M.; Shepherd, David; Shirangi, Troy R.; Simpson, Jeffrey H.; Truman, James W.; Tuthill, John C.; Williams, Darren W.

doi:10.1101/122952

Cited by 21 publications

(17 citation statements)

References 48 publications

(44 reference statements)

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“…Five of these bundles totaling 42 neurons exited the VNC through the ProLN (Fig. 3B), five bundles totaling 12 neurons through the prothoracic accessory nerve (ProAN), six bundles totaling 11 neurons through the ventral prothoracic nerve (VProN), and two bundles totaling four neurons through the dorsal prothoracic nerve (DProN; nomenclature from Court et al 2017). We found a single bundle containing 29 motor neurons (cyan in Fig.…”

Section: Cell Type-specific Clustering Of Sensory and Motor Neuron Axonsmentioning

confidence: 91%

“…After aligning the acquired images into a three-dimensional image volume (see Methods), we searched for axon bundles leaving the VNC as peripheral nerves. We found all previously described nerves that innervate the legs, wings, halteres, and neck (Court et al, 2017;Power, 1948). For individual neurons passing through each nerve, we built skeleton models of their projection patterns within the VNC.…”

Section: Motor and Sensory Neurons Occupy Distinct Domains Within Permentioning

confidence: 99%

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Reconstruction of motor control circuits in adultDrosophilausing automated transmission electron microscopy

Maniates-Selvin

Hildebrand

Graham

et al. 2020

Preprint

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Many animals use coordinated limb movements to interact with and navigate through the environment. To investigate circuit mechanisms underlying locomotor behavior, we used serial-section electron microscopy (EM) to map synaptic connectivity within a neuronal network that controls limb movements. We present a synapse-resolution EM dataset containing the ventral nerve cord (VNC) of an adult female Drosophila melanogaster. To generate this dataset, we developed GridTape, a technology that combines automated serial-section collection with automated high-throughput transmission EM. Using this dataset, we reconstructed 507 motor neurons, including all those that control the legs and wings. We show that a specific class of leg sensory neurons directly synapse onto the largest-caliber motor neuron axons on both sides of the body, representing a unique feedback pathway for fast limb control. We provide open access to the dataset and reconstructions registered to a standard atlas to permit matching of cells between EM and light microscopy data. We also provide GridTape instrumentation designs and software to make large-scale EM data acquisition more accessible and affordable to the scientific community.

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Section: Cell Type-specific Clustering Of Sensory and Motor Neuron Axonsmentioning

confidence: 91%

Section: Motor and Sensory Neurons Occupy Distinct Domains Within Permentioning

confidence: 99%

Reconstruction of motor control circuits in adultDrosophilausing automated transmission electron microscopy

Maniates-Selvin

Hildebrand

Graham

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…For example, the ~50 motor neurons (MNs) that innervate each leg in adult Drosophila are born from invariant NB lineages and have highly stereotyped birthdates and morphologies (Baek and Mann, 2009; Brierley et al, 2012). These stereotyped morphologies can be seen both in the muscles these motor neurons target and in their elaborate dendritic arbors that establish a very large number of synapses in dense neuropils present in each thoracic neuromere of the ventral nerve cord (VNC) (Court et al, 2017). Although each motor neuron morphology is thought to be determined by unique combinations of morphology transcription factors (mTFs; Enriquez et al, 2015), how these stereotyped morphologies form the correct synaptic connections with interneurons and sensory neurons is not understood.…”

Section: Introductionmentioning

confidence: 99%

Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila

Enriquez

Rio

Blazeski

et al. 2018

Neuron

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SUMMARY In vertebrates and invertebrates, neurons and glia are generated in a stereotyped manner from neural stem cells, but the purpose of invariant lineages is not understood. We show that two stem cells that produce leg motor neurons in Drosophila also generate neuropil glia, which wrap and send processes into the neuropil where motor neuron dendrites arborize. The development of the neuropil glia and leg motor neurons is highly coordinated. However, although motor neurons have a stereotyped birth order and transcription factor code, the number and individual morphologies of the glia born from these lineages are highly plastic, yet the final structure they contribute to is highly stereotyped. We suggest that the shared lineages of these two cell types facilitate the assembly of complex neural circuits and that the two birth order strategies—hardwired for motor neurons and flexible for glia—are important for robust nervous system development, homeostasis, and evolution.

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“…For example, the ~50 motor neurons (MNs) that innervate each leg in adult Drosophila are born from invariant NB lineages and have highly stereotyped birthdates and morphologies (Baek and Mann, 2009;Brierley et al, 2012). These stereotyped morphologies can be seen both in the muscles these MNs target and in their elaborate dendritic arbors that establish a very large number of neural synapses in dense neuropils present in each thoracic neuromere of the ventral nervous system (VNS) (Court, et al, 2017). Although each MN morphology is thought to be determined by unique combinations of morphology transcription factors (mTFs; (Enriquez et al, 2015)), how these stereotyped morphologies form the correct synaptic connections with interneurons and sensory neurons is not understood.…”

Section: Introductionmentioning

confidence: 99%

Differing strategies used by motor neurons and glia to achieve robust development of an adult neuropil in Drosophila

Enriquez

Blazeski

et al. 2017

Preprint

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2 SummaryIn both vertebrates and invertebrates, neurons and glia are generated in a stereotyped order from dedicated progenitors called neural stem cells, but the purpose of invariant lineages is not understood. Here we show that three of the stem cells that produce leg motor neurons in Drosophila also generate a specialized subset of glia, the neuropil glia, which wrap and send processes into the neuropil where motor neuron dendrites arborize. The development of the neuropil glia and leg motor neurons is highly coordinated. However, although individual motor neurons have a stereotyped birth order and transcription factor code, both the number and individual morphologies of the glia born from these lineages are highly plastic, even though the final structure they contribute to is highly stereotyped. We suggest that the shared lineages of these two cell types facilitates the assembly of complex neural circuits, and that the two different birth order strategies -hardwired for motor neurons and flexible for glia -are important for robust nervous system development and homeostasis.

show abstract

A Systematic Nomenclature for theDrosophilaVentral Nervous System

Abstract: 15Insect nervous systems are proven and powerful model systems for neuroscience research with

Cited by 21 publications

References 48 publications

Reconstruction of motor control circuits in adultDrosophilausing automated transmission electron microscopy

Reconstruction of motor control circuits in adultDrosophilausing automated transmission electron microscopy

Differing Strategies Despite Shared Lineages of Motor Neurons and Glia to Achieve Robust Development of an Adult Neuropil in Drosophila

Differing strategies used by motor neurons and glia to achieve robust development of an adult neuropil in Drosophila

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